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| Cloning and Sequence Analysis of EIN3 Gene from Cucumis sativus with Different Sex Expression |
| Liu Xiaohong1，Chen Huiming1，2，Xu liang2，et al. |
| 1Hunan Vegetable Research Institute，Changsha 410125 |
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Abstract A pair of oligonucleotide primers was designed from conserved domain of EIN3 gene family.PCR amplifications were performed on different sex genotype cucumber DNA template and produced 6 fragments of 725 bp，respectively.After cloning and sequencing on CsEIN3 of different sex genotype cucumbers，BLAST and DNAMAN analysis found 14 single nucleotide polymorphisms（SNPs）in coding region，about one average 51 bp，selection rating 1.96 %，3 mutation in enzyme digested site，eight SNPs mutation cause amino acid sequence variation.One set of stable RFLP-BfmⅠenzyme digested reaction system was established.By digesting DNA from different sex genotypes，a specific enzyme digest band in WI1983H was found.
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Received: 15 June 2008
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