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| Study on Genetic Relationship of Cultivated Radish（Raphanus sativus L.）Germplasms by Morphological and AFLP Markers |
| Han Taili，Li Kefeng，Tan Jinxia |
| （Vegetable Research Institute，Weifang Academy of Agricultural Sciences，Weifang 261061） |
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Abstract AFLP and morphological analysis were carried out on 33 radish（Raphanus sativus L.）germplasms.Eight primer combinations were selected for using in characterizing the radish collection.Differences in number，intensity，and size of amplification products were observed depending on the primer combination，ranging between 35 and 56 bands per pair with an average of 45.2 bands.The polymorphism rate was 42.10 %，which indicates abundant genetic diversity existing among the cultivated radish germplasms.These germplasms were clustered into four groups by morphological analysis and AFLP，respectively.Rs14 was single in one cluster which suggests it is a unique germplasm for its purple flesh.The clustering patterns of other materials for AFLP and morphological data were similar.And in general，the others were divided into three groups according to their skin colors：green radish，white radish and red radish.
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Received: 14 March 2008
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