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Abstract Abstract：Tomato spotted wilt virus（TSWV）was identified by RT-PCR in suspected pepper virussamples collected from 6 regions in Guizhou Province，and the N gene sequences of TSWV in different regionswere amplified by molecular cloning．The results showed that TSWV were found in chilies from Guiyang，Anshun，Weng’an，Fuquan，Changshun and Huishui in Guizhou．After sequences aligning，the nucleotideand amino acid identities of the strains isolated from Guizhou were 95.6%-99.7% and 97.1%-100.0%，respectively．Phylogenetic tree analysis suggested that TSWV strains isolated from pepper in Fuquan，Huishuiand Changshun were closely related to those in Yunnan and Shandong．The TSWV strains isolated frompeppers in Anshun，Guiyang and Weng’an were relatively closed to the strains isolated from tomato，pepper，tobacco，celery and Herb of Spanish needles in Yunnan and lettuce in Jiangsu．The study indicated that therewere two TSWV lines in Guizhou pepper，which were certainly correlated with those isolates from varioushosts in different regions in China．
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Received: 12 June 2023
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