Taking tomato‘ Jinpeng No.11’ as experimental material，this paper studied the physical and chemical properties of different ratio of coconut coir，peat，vermiculite and perlite nursery substrate，and their effects on indexes of tomato seedling growth，physiological and biochemical properties．The results showed that different matching ratio of coconut coir and vermiculite could significantly improve the physical and chemical properties of substrate．Integrating all important physiological and biochemical indexes，including plant growth vigor，G value，total fresh plant weight，chlorophyll content，soluble protein，soluble sugar and root activity，etc.，the substrate with coconut coir and vermiculite 5 V∶5 V matching ratio was the most beneficial for the growth of tomato plug seedlings .