In order to explore the effects of Verticillium dahiliae allergen Asp f2-like（VdAL） on storage quality of commercial cucumber seedling，this paper takes cucumber seedlings as test material，and conducts 3 spraying processing with different concentrations，and analyzes the quality-related indicators of cucumber seedlings before storage and 4 days after storage and also the recovery growth indicators 3-6 days after planting of seedlings. The results show that 1.25 g·L^-1 VdAL treatment can significantly enhance the tolerance of cucumber seedlings to storage and transportation. Compared with other treatments，the concentration of this treatment makes the cucumber seedlings have very stable chlorophyll contents during storage period，and have fast growth after planting. These facts suggest that foliage spraying with suitable VdAL concentration is helpful for upgrading the storage quality of commercial cucumber seedlings.