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| Cloning of Coat Protein Gene of Tomato Yellow Leaf Curl Virus Strain from Shouguang Region of Shandong Province and Its Expression in E. coli BL21（DE3） |
| QIAO Ning1，LI Mei-qin1*，GUO Bao-tai2，LIU Yong-guang1，PEI Hua-li1，ZHU Xiao-ping3 |
| 1Institute of Vegetables and Flowers，Weifang College of Science and Technology，Shouguang 262700，Shandong，China|2College of Life Science，Qingdao Agricultural University，Qingdao 266109，Shandong，China|3College of Plant Protection，Shandong Agricultural University，Tai’an 271018，Shandong，China |
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Abstract The PCR test was carried out on tomato（Lycopersicon esculentum Mill．）leaves which were infected by TYLCV in the greenhouse in Shouguang region of Shandong Province using the prime PA/PB．The results showed the yellow leaf curl disease found in Shouguang was caused by TYLCV．A DNA fragment（770 bp in length）encoding TYLCV coat protein（TYLCV-CP）was amplified and inserted into pEASY-T1 Simple vector．After double digestion with Xho I and EcoR I，the fragment was inserted into vector pET-32a．A 50 kD recombinant protein of TYLCV-CP from isolate of Shouguang highly expressed by IPTG in E. coli BL21（DE3）showed a high antigenic activity．
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Received: 20 September 2011
Published: 07 January 2012
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