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| Difference of SUTI Gene Expression in Tomato under Different Cultural Measure by Quantitative RT-PCR（qRT-PCR）Reactions |
| XIE Xiao-yu1,2，YIN Yong-gen2，N. Fukuda2 |
| 1College of Agronomy and Biotechnology，Southwest University，Chongqing 400716，China；2Gene Research Center，Graduate School of Life and Environmental Sciences，University of Tsukuba，Tsukuba 305-8572，Japan |
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Abstract To study the differential expression of sucrose transporter（SUT1）gene under different cultural method in tomato（Lycopersicon esculentum Mill.），the experiment was performed by quantitative real time PCR（qRT-PCR）reactions．The result show that the relative expression level of SUT1 gene in leaves and stalk was higher than in fruit．The relative expression level of SUT1 gene in leaves，stalk and fruit under EC 1.8 was higher than under EC 3.0．When under bigger culture density，it was higher than under lower culture density．It was higher without clipping than with 1/3 clipping and 1/2 clipping．Influence of variety on SUT1 relative expression level was very complex．That of‘Laibangsuo’in leaves was higher than‘Lirong’，while it was contrary to the former in stalks and fruits．This indicated that‘Laibangsuo’was more favorable for sugar transportation and accumulation in fruit than‘Lirong’．There was positive correlation between expression level of SUT1 gene in leaves and yield of single tomato plant．
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Received: 12 February 2011
Published: 11 April 2011
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