Evaluation on Resistance of Botrytis cinerea Isolates from Vegetable Crops to Different Types of Fungicides
SHI Yan-xia1,TANG Ming1,2,JIN Zhi-wen1,XIE Xue-wen1,CHAI A-li1,LI Bao-ju1*
(1Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2 Plant Protection College,Shenyang Agricultural University,Shenyang 110866,Liaoning,China)
For investigating the development situation of Botrytis cinerea resistance to different types of fungicides,85 isolates obtained from vegetables in North China were tested for their sensitivity to benzimidazoles (carbendazim),dicarboximides(procymidone),N-phenylanilines(diethofencarb),anilinopyrimidines (pyrimethanil),amides( boscalid) and phenylpyrroles(fludioxonil). The results revealed that Botrytis cinerea in North China have developed different levels of resistance to 6 different types of fungicides,and the resistances to common fungicides including pyrimethanil,boscalid and fludioxonil were spread quickly in managing the grey mold. At the same time,Botrytis cinerea had generated a high level of resistance to carbendazim,procymidone,diethofencarb and the total resistance frequency were 72.94%,51.76%,69.41%,respectively. The frequency of double resistance to carbendazim and diethofencarb was 51.76%,whereas triple-resistance to carbendazim,procymidone and diethofencarb was 34.12%. Moreover,high resistance level was also detected in the tested Botrytis cinerea to pyrimethanil and boscalid,2 common fungicides for controlling gray mold,with resistant frequency 64.71% and 65.88%,respectively. Resistant frequency to the new fungicides fludioxonil was 36.47%. Forty multiple-resistance type were found in this research,and 32 types have not been reported yet. One strain that was sensitive to the tested 6 fungicides was reported,with frequency of 1.18%,while 5 strains resistant to the 6 fungicides were verified,with frequency of 5.88%. We suggest that long-term repeated use of a single fungicide should be avoided in controlling grey mold,and application of multiple agents should be recommended so as to prolong the service life of fungicide.
石延霞1 唐 明1,2 晋知文1 谢学文1 柴阿丽1 李宝聚1*. 蔬菜作物灰葡萄孢菌对不同类型杀菌剂的抗性评价[J]. 中国蔬菜, 2016, 1(3): 60-.
SHI Yan-xia1,TANG Ming1,2,JIN Zhi-wen1,XIE Xue-wen1,CHAI A-li1,LI Bao-ju1*. Evaluation on Resistance of Botrytis cinerea Isolates from Vegetable Crops to Different Types of Fungicides. China Vegetables, 2016, 1(3): 60-.