SNP-based Molecular Assay for Cucumber Hybrid Seed Purity Iden- tification by Pyrosequencing
LAN Qing-kuo1，ZHANG Gui-hua2，WANG Yong1*，ZHAO Xin1，ZHU Zhu1，CUI Xing-hua2，GUO Yong-ze1，CHENG Yi1
1Central Lab of Tianjin Academy of Agricultural Sciences，Tianjin 300381，China|2Cucumber Research Institute of Tianjin Kernel Agricultural Science and Technology Co.，Ltd.，Tianjin 300192，China
Abstract：For the purpose of testing cucumber（Cucumis sativus L.）hybrid purity，a SNP site was screened by high resolution melting（HRM）from 33 commercial cucumber cultivars，whose polymorphism information（PIC）were 0.401．We established the purity test method based the SNP，which was genotyped by pyrosequencing．The method was applied in purity test of‘Youyi’cucumber cultivar．The result indicated that 90 were pure hybrid seeds，and the purity was 96.7%，which is coincide with the results of CLA0 and SSR．Overall，the method is fit for purity test of cucumber hybrid and the SNP-pyrosequencing test model is suitable for distinctness，uniformity and stability（DUS）analysis．
