Suppression Mechanism of Meloidogyn incognita Infection on Cucumber by Soil Amendments
WANG Ze1, LIU Qi-zhi1*, ZHOU Hai-ying2, LIANG Li-ping2
1Key Laboratory for Biological Control, Ministry of Agriculture, Laboratory of Entomology and Nematology-LEN, College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China; 2Beijing Key Laboratory of Crop Genetic Improvement, College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China
Abstract：The observation and comparing of soil amendment（SA, the main ingredients come from insect body and its metabolic production, developed by authors’laboratory）effect on histopathology features in cucumber（Cucumis sativus L.）‘Qianqiu No.1’radicle tissue infected by root-knot nematodes（RKN, Meloidogyn incognita）with methods of acid fuchsin staining and paraffin section were carried out in this study. The results demonstrated that after 7 days of RKN inoculation and SA treatment, the cucumber growth indicators，giant cell（GC）quantity and morphology had no significant differences with or without SA. However，after 15 days, the total area of cucumber leaves, over-ground part of plant fresh weight and root length in SA treatment were increased about 1/3; the number of root-knot per 10 cm root length was reduced down 1/2. GC became wizened and vacuolated with SA treatment and became hypertrophic without SA treatment. It showed that SA does not prevent RKN finding and infecting root system, but effect on wizened and vacuolated GC formation, which cause RKN food shortage in the GC, by which, the crop capacity against RKN was enhanced.
