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2020 Vol.1 Issue.10,Published 2020-10-01
1
Hot!
2020 Vol. 1 (10): 1-8 [Abstract] ( 218 ) [HTML 1KB] [ PDF 2663KB] ( 1151 )
9
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2020 Vol. 1 (10): 9-12 [Abstract] ( 193 ) [HTML 1KB] [ PDF 1369KB] ( 405 )
13
2020 Vol. 1 (10): 13-17 [Abstract] ( 177 ) [HTML 1KB] [ PDF 943KB] ( 597 )
18 Research Progress on Salt Tolerance in Cucurbita
WANG Wei-qi1，ZHANG Meng2，QIN Zhao-chen1，MA Wei2，SUN Ting-zhen2，SHI Yu-zi2，DUAN Ying2*，WANG Chang-lin2* Hot!
Abstract：Soil salinization is one of the main abiotic stresses in vegetable production．Pumpkin
（Cucurbita spp.）displayed strong tolerance to salt stress and was used as rootstock in grafting cultivation of
cucumber，melon and watermelon．Selection of pumpkin varieties with salt tolerance is helpful to improve
the yield and economic benefits of pumpkin and Cucurbitaceae grafted vegetables．This paper summarized the
research progress on evaluation methods for salt tolerance of Cucurbita and salt-tolerant Cucurbita germplasm
screening，physiological mechanism in forming Cucurbita salt tolerance，inheritance of Cucurbita salt
tolerance traits and related salt tolerance genes in Cucurbita．Furthermore，the paper also prospected the future
research direction，hoping to provide more theoretical references for understanding the regulatory mechanism
of pumpkin salt tolerance，and selection of salt tolerant varieties．
2020 Vol. 1 (10): 18-26 [Abstract] ( 307 ) [HTML 1KB] [ PDF 1042KB] ( 658 )
27 Comparative Analysis and Application of Rapid Detection on Potato Soil-borne Pathogens
LI Lei，ZHAO Yu-rong，XIE Xue-wen，SHI Yan-xia，CHAI A-li，LI Bao-ju*
Potato soil-borne diseases are becoming increasingly serious．Rapid detection on pathogenic
bacteria is an important basis for early diagnosis，predictive monitoring，and comprehensive control of the
diseases．This paper comprehensively compared the characteristics of conventional PCR，nested PCR，realtime
quantitative PCR，multiplex PCR and LAMP technology in sensitivity and specificity，and evaluated their
merits and demerits in detecting potato soil-borne diseases．The paper also summarized the primer information
of various diseases detection reports and advantages of this detection system．At the same time，the paper
prospected the applying future of each technology，aiming at obtaining an overall understanding about the rapid
detection methods and their development tendency on potato soil-borne diseases．
2020 Vol. 1 (10): 27-34 [Abstract] ( 226 ) [HTML 1KB] [ PDF 1055KB] ( 616 )
35 Analysis on Cloning and Expression of ACC Synthase（CqACS）Gene from Benincasa hispida
PENG Qin2，WANG Min1，3，PENG Qing-wu1，JIANG Biao1，3，LIN Yu-e1，LIU Wen-rui1，3，LIANG Zhao-jun1，LI Ling2，HE Xiao-ming1，3 Hot!
In order to find out the relation between ACC synthase and female chieh-qua，this study took
female inbred line（A36）and normal monoacious inbred line（SX）as material，gained CqACS gene through
coloning，then conducted homologous sequence comparative analysis with the known CqACS gene；and
constructed evolutionary tree．The expression profile of CqACS in seedling leaf blades of A36 was analyzed
after treatment with gibberellin（GA3）by qRT-PCR on CqACS gene．The female flower rate within 20 nodes
was carried out statistic analysis before and after gibberellin treatment．The results showed that the length of
CqACS gene in A36 of female inbred line was 1 318 bp and that in normal monoecism selfing line in SX was
1 637 bp．After comparative analysis，we found that SX in 3 bp base element：91，97 bp and 117 bp were
more than detected in A36，and only 2 basic group differences were found between them．The homologous
sequence comparison revealed that CqACS gene in A36 had the highest homology with Cit-ACS1 gene in
watermelon，reaching 95.31%．And the homology with cucumber Cs-ACS1 was 91.31%．CqACS gene in SX
had the homology with Cit-ACS1 gene in watermelon，reaching 78.04%，and the homology with cucumber
Cs-ACS1 was 76.06%．After GA3 treatment，the average female flower rate（within 20th node order）of GA3
treatment group in A36 was 45%．While，that of the contrast group was 90%．qRT-PCR analysis discovered
that the expression quantity of CqACS gene after GA3 treatment was significantly up-regulated．Compared with
the contrast group．Moreover，4 h after the third time treatment the up-regulated range was the largest．
2020 Vol. 1 (10): 35-41 [Abstract] ( 266 ) [HTML 1KB] [ PDF 1649KB] ( 371 )
42 Genetic Diversity Analysis of Eggplant Germplasm Resources Based on InDel Markers
ZHANG Qiang-qiang1，3，JIANG Hai-kun1，2，WANG Yan1，2，LIANG Sai1，4，SUI Yi-hu3*，JIA Li1，2*，FANG Ling1，2，ZHANG Qi-an1，2，DONG Yan-xiang1，2
In this experiment，19 pairs of InDel markers were used to study the genetic diversity of 46
germplasm resources of eggplant from home and abroad．The results showed that the PIC values of 19 pairs
of InDel markers ranged from 0.48-0.66，with 0.59 mean value．The genetic similarity coefficient of 46
eggplant germplasm resources was between 0.32-1.00 with 0.70 mean value，indicating little genetic difference
between 46 eggplant germplasm resources and with relatively narrow genetic basis．UPGMA method was
used for clustering analysis，and 46 germplasm resources of eggplant were divided into 4 groups at the genetic
similarity coefficient of 0.66．The clustering result had certain relevance with leaf color，corolla color and fruit
characters，while had little relevance with area of source．
2020 Vol. 1 (10): 42-47 [Abstract] ( 175 ) [HTML 1KB] [ PDF 1368KB] ( 537 )
48 Changes of Endogenous Hormone Content in Watermelon Varieties with Different Branching Forms During Their Growth Process
YANG Yong-gang，ZHANG Hua-sheng，LI Xiao-fang，SU Yong-quan
 Taking multi-branch watermelon variety ‘Longkang No.9’ and no branch watermelon variety
‘Wuchazao’ as experimental materials，this paper studied and analyzed the changes of endogenous hormone
content，including Indole-2-carboxylic acid（ICA），indole-3-acetic acid（IAA），zeatin（Z），dihydrozeatin
（DHZ），abscisic acid（ABA），gibberellin（GA1，GA3，GA4 and GA7） by HPLC-MS/MS during
watermelon growth period．The results showed that the contents of ICA，ICA+IAA in watermelon leaves of no
branch variety were significantly higher than that of multi-branch variety during vine spreading period and fruitexpanding
early stage．During the whole growth period，the contents of Z，DHZ+Z in no branch variety were
lower than that of multi-branch variety．Furthermore，the ratios of （ICA+IAA）/（Z+DHZ），（ICA+IAA）/
ABA，（ICA+IAA）/（GA1+GA3+GA4+GA7） in no branch variety were significantly higher than that of multibranch
variety during vine spreading period and early stage of fruit expanding．Thus，it could be seen that
higher contents of ICA could inhibit the branching of watermelon side shoots；while the higher content of DHZ
and Z could promote the form of watermelon lateral branches．The higher ratios of（ ICA+IAA）/（Z+DHZ），
（ICA+IAA）/ABA，（ICA+IAA）/（GA1+GA3+GA4+GA7） were harmful to the formation of water melon
branches．
2020 Vol. 1 (10): 48-54 [Abstract] ( 191 ) [HTML 1KB] [ PDF 1341KB] ( 471 )
55 Comparison and Physiological Traits of Main Agronomic Characters of Nuclear#br# Sterile Lines in Pepper
LIANG Sai1，2，JIA Li1，3，WANG Yan1，3，ZHANG Qiang-qiang1，3，CHEN You-gen2*，JIANG Haikun1，3*，FANG Ling1，3，ZHANG Qi-an1，DONG Yan-xiang1，3
Taking 2 pepper nuclear male sterile lines‘GMSDS-702A’and‘GMSDS-702B’as
experimental material，this paper conducted investigation on their main agronomic traits，and measured the
contents of relevant physiological indicators at different development stages of flower buds．The results showed
that the vegetative growth of sterile line plant was relatively vigorous．The plant height and spread width were
significantly higher than that of the fertile line．The anthers of the sterile line were shriveled，with browned
surface，the tissue structure was hardened，and the anthers were tightly closed and could not form normal
pollen grains．The contents of soluble sugar，soluble protein，free proline，and malondialdehyde were all
different with various degrees at different development stages．At the stage of microspore mother cell，the
contents of soluble sugar and free proline in sterile lines were reduced by a big margin，and the content of
malondialdehyde was at a higher level．Those of，fertile lines were on the contrary．It might have a certain
inner link with the abortion of microspore．
2020 Vol. 1 (10): 55-61 [Abstract] ( 169 ) [HTML 1KB] [ PDF 2287KB] ( 373 )
62 Obtain and Identification of F1 hybrid of Solanum melongena L. and Solanum khasianum C. B. Clarke
XUE Jin-yan，LIU Yi-yong，WU Jun-xiang，XU Zhao-can，ZHANG Yu，CHENG Yu-fu，YANG Xu
Solanum khasianum C. B. Clarke is an affinis wild eggplant species，possessing valuable
genes resisting both biotic stress and abiotic stress．Taking 10 cultivated eggplants as female parent，
proceeding hybridization with Solanum khasianum C. B. Clarke，and adopting pollination methods as once
pollination，repeating pollination，style cutting back，mixture pollination and pollination after grafting，
etc．，this study had obtained the best way for interspecies hybridizatyon between Solanum melongena L. and
Solanum khasianum C. B. Clarke．The results showed that adopting pollination after grafting could increase
hybrid fruit setting rate，and obtain F1 hybrid between L81 and Solanum khasianum C. B. Clarke．SSR
molecular identification was conducted on F1 plant at seedling stage and the F1 seed was proven to be the seed of
interspecific hybridization between Solanum melongena L. and Solanum khasianum C. B. Clarke．
2020 Vol. 1 (10): 62-67 [Abstract] ( 221 ) [HTML 1KB] [ PDF 1749KB] ( 396 )
68 Identification of Hyacinth Bean Sclerotinia Pathogen and Its Biological Characteristics
GU Yu-yang1，WANG Li-na2，YUAN Juan3，ZHANG Yi1，YAO Lu-ming1，MA Xiao-hong1，WANG Biao1*，WU Tian-long1
In recent years，a sclerotinia disease similar to symptoms of the other crops has broken out
at the main hyacinth bean producing area in Shanghai Pudong district．Through conducting mophological
observation，pathogenicity identification on separated and purified pathogenic bacteria，analyzing molecular
biology characteristics，we confirmed that the pathogen causing this disease was Sclerotinia sclerotiorum
（Lib．）de Bary．This pathogen could infect the whole plant of hyacinth bean，including its root，stem，
leaf，flower and pod．We had also observed the formation of its sclerotia．This study discovered that the
best growth carbon source for indoor seperation and culture of Sclerotinia sclerotiorum was fructose at 22 ℃，
pH 5．Proper growth conditions under stress were in favour of forming sclerotia．There were differences
existed in ITS rDNA sequences of Sclerotinia sclerotiorum from hyacinth bean，soybean and pakchoi．
Comparing Sclerotinia sclerotiorum originating from those different sources，we found that those strains
belonged to different fusion groups，and they had discrepant pathogenicities to different hosts，but all of them
could cause diseases．
2020 Vol. 1 (10): 68-76 [Abstract] ( 186 ) [HTML 1KB] [ PDF 2542KB] ( 488 )
77 Analysis on Quantity，Distribution Characteristics and Utilization as Resource Manure Potential of Different Kinds Vegetable Wastes in Hebei Province
DUAN Ya-jun1，ZHANG Hui1，LYU Shi1，ZHANG Yu-kun1，WANG Xiao-min1，NING Guo-hui1，YANG Zhi-xin1，2*
Taking 8 kinds of main vegetable wastes in 11 prefecture-level cities of Hebei Province as study
object，the paper estimated the quantity of different vegetable wastes resources in Hebei Province by grassgrain
ratio．The results showed that the quantity of vegetable waste resources in Hebei Province increased at
an average rate of 42 600 t · a-1 from 2011-2016，reaching 2.30 million t in 2016．Among which，cabbage
（578 700 t），Chinese cabbage（434 200 t） and solanaceous vegetables（299 800 t） had the highest quantity
of vegetable waste resources，increasing by 100.98%，50.80% and 4.12%，respectively compared with
the mean value，followed by tubers，beans and melon vegetables．Those of leaf and bulb vegetables were
the lowest．The quantity of waste resources was distributed unevenly among each prefecture-level city，
with 50.57%-82.52% coefficient variation．Tangshan（408 100 t），Shijiazhuang（351 700 t），Baoding
（297 800 t） and Zhangjiakou（279 600 t） had the maximum quantities，accounting for 58.04% of the whole
province．Utilization as resource manure of all these vegetable wastes in the province was equivalent to indirect
application of 45.15 kg · hm-2 pure nitrogen，27.75 kg · hm-2 pure phosphorus，44.85 kg · hm-2 pure potassium
and 1 089.15 kg · hm-2 of organic matter per 667 m2 farmland；and equivalent to 6.68% of the annual amount
of chemical fertilizer and 2.992 million t of organic fertilizer，among which cabbage，Chinese cabbage and
solanaceous vegetables had the highest nitrogen，phosphorus and potassium nutrients，accounting for 57.73%
of the 8 kinds vegetables．The organic matter content in tubers vegetables was the highest．The quantity of
vegetable waste resources in the whole province showed a steady upward trend from 2011-2016．There were
significant differences in the quantities of all kinds of vegetable wastes and nutrients in different regions，among
which vegetable yield and waste coefficient had become important factors affecting the difference of vegetable
waste resources quantities．
2020 Vol. 1 (10): 77-82 [Abstract] ( 204 ) [HTML 1KB] [ PDF 1244KB] ( 353 )
83 A New Hot Pepper F1 Hybrid of Mid-maturity —‘Ruizhi’
WANG Chao-lian，DING Hai-feng，HE Wei-ming
‘Ruizhi’is a new tapered pepper F1 hybrid developed by crossing thick cow-horn pepper stable
inbred line‘11-22’as female parent and pimento stable inbred line‘11-431’as male parent．It is of midmaturity．
The initial flower mostly sets on the 9th node．The plant has strong growth vigor．Its continuous
fruit setting ability is good．The fruit is in nice order．The upper fruits and down fruits have good consistency．
The fruit is about 17 cm in length，5 cm in shoulder width and 0.45 cm in pulp thickness with 2 or 3 lobes．
The single fruit weight is about 130 g．Its fruit has smooth surface with shining glossiness．The young fruit
is dark green in color．The matured one is dark red in color and changes color quickly with good hardness．
It tastes very hot．Its resistance to virus disease，bacterial wilt and stem rot in fields is stronger than that of the
contrast‘Tianza No.1’．At earlier stage，it can yield about 37.5 t · hm-2，and the total yield is about 63 t ·
hm-2．It is suitable for planting in protected and open fields in Yunnan and Sichuan Provinces．
2020 Vol. 1 (10): 83-85 [Abstract] ( 171 ) [HTML 1KB] [ PDF 2533KB] ( 476 )
86 A New Melon F1 Hybrid —‘Chujin No.1’
WANG Yun-qiang，DAI Zhao-yi，YI Li-cong，GONG Yu，WANG Shu-jing
‘Chujin No.1’is a new melon F1 hybrid of medium and late maturity developed by crossing
inbred line‘TH607’as female parent and inbred line‘TH605’as male parent．Its whole growth period is
about 103 days，and the fruit development period is 42-45 days．The plant growth vigor is strong．Its branch
ability is medium．The leaf is green in color．The fruits mainly bear on lateral branches．The fruit is of round
shape and pericarp is yellow with dark green plaques．The average single fruit weight is about 1.5 kg．Its flesh
is white in color with about 3.7 cm thickness and soft quality．The content of center soluble solid is about 16%．
The fruit quality is fine with good taste．In fields，its resistance to powdery mildew and downy mildew was
amount to that of the contrast‘Yilishabai’．Its yield under plastic greenhouse by vine hanging cultivation was
about 37.5 t · hm-2．It is suitable for facility cultivation in spring and autumn at the middle and lower reaches of
the Yangtze River．
2020 Vol. 1 (10): 86-88 [Abstract] ( 158 ) [HTML 1KB] [ PDF 2544KB] ( 432 )
89 A New Tomato F1 Hybrid with Resistant to TYLCV —‘Longfan No.1’
HOU Fu-en，HAO Ke-xing*，SU Dong-tao，ZHANG Tao，WANG Ming，ZHANG Man，HOU Dong-ying
Abstract：‘Longfan No.1’is a new tomato F1 hybrid developed by crossing inbred line‘TMX2511-2’as
female parent and‘TMX2535-4’as male parent．It is of indeterminate growth type．The plant has strong
growth vigor．The first female flower node order was at 8-9th node．The fruit is red in color and of oblate
shape with 6.5-7.0 cm vertical diameter，and 8.0-8.5 cm transverse diameter．The average single fruit weight
is about 220 g．The fruit is of medium firmness．Its young fruit has no green shoulder．The fruit soluble solid
content is 5.9%，lycopene content is 0.035 mg · g-1，VC content is 0.167 mg · g-1．It can generally yield about
127.5 t · hm-2．It is highly resistant to TYLCV，resistant to leaf mold，and moderate resistant to CMV and root
knot nematode．This variety is suitable for planting under plastic greenhouse in early spring and late autumn at
Lyuliang，Xinzhou，Jinzhong，Datong，Changzhi of Shanxi Province．
2020 Vol. 1 (10): 89-92 [Abstract] ( 207 ) [HTML 1KB] [ PDF 2726KB] ( 428 )
92 New Erythropalum scandens Blume Varieties with Medicinal and Edible Vegetable —‘Guichicangteng No.1’and‘Guichicangteng No.2’
ZHANG Shang-wen，LI Ting，SHI Qian，PAN Ying-nan，YU Wei-hua，MENG Ping，ZHANG Xiang-jun*
Abstract：‘Guichicangteng No.1’and‘Guichicangteng No.2’are excellent strains of new medicinal and
edible vegetable varieties screened from the wild resources in Guangxi and bred mainly by rapid propagation via
tissue culture，supplemented by cutting propagation．The tender shoots of‘Guichicangteng No.1’are green
in color，with a yield of 28.5 t · hm-2．The tender shoots of‘Guichicangteng No.2’are red in color．Its flavor
is more rich than that of‘Guichicangteng No.1’．‘Guichicangteng No.2’can yield 25.5 t · hm-2．These 2
varieties all have axillary tendril and slender branches，on which there are indistinct stripes．The leaves change
from papery to thick papery or subleathery and were 8-20 cm in length，4-15 cm in width．The fresh tender
buds are about 15 cm in length with special flavor．The contents of protein，fat，coarse fiber，calcium，
phosphorus，VC and total amino acid content are higher than that of normal vegetables．Three years after field
planting，these 2 varieties will enter into a high-yielding period．They are suitable for open fields cultivation in
Guangxi and Southwestern areas，and protected cultivation in northern regions．
2020 Vol. 1 (10): 92-95 [Abstract] ( 192 ) [HTML 1KB] [ PDF 2673KB] ( 397 )
96
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2020 Vol. 1 (10): 96-99 [Abstract] ( 170 ) [HTML 1KB] [ PDF 1353KB] ( 392 )
99
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2020 Vol. 1 (10): 99-102 [Abstract] ( 190 ) [HTML 1KB] [ PDF 2695KB] ( 583 )
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2020 Vol. 1 (10): 103-105 [Abstract] ( 200 ) [HTML 1KB] [ PDF 1822KB] ( 431 )
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2020 Vol. 1 (10): 106-108 [Abstract] ( 176 ) [HTML 1KB] [ PDF 906KB] ( 491 )
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2020 Vol. 1 (10): 109-110 [Abstract] ( 167 ) [HTML 1KB] [ PDF 1073KB] ( 246 )
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2020 Vol. 1 (10): 111-112 [Abstract] ( 207 ) [HTML 1KB] [ PDF 1249KB] ( 476 )
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