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2020 Vol.1 Issue.7,Published 2020-07-01
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2020 Vol. 1 (7): 1-6 [Abstract] ( 224 ) [HTML 1KB] [ PDF 4491KB] ( 657 )
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2020 Vol. 1 (7): 7-12 [Abstract] ( 219 ) [HTML 1KB] [ PDF 1514KB] ( 1010 )
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2020 Vol. 1 (7): 12-14 [Abstract] ( 180 ) [HTML 1KB] [ PDF 1359KB] ( 393 )
15 Research Progress on Cucumber Breeding with Resistance to Grey Mould
DAI Zhuo-nan，MIAO Han，LI Bao-ju，SHI Yan-xia，BO Kai-liang，DONG Shao-yun，GU Xingfang，ZHANG Sheng-ping Hot!
Cucumber grey mould is caused by Botrytis cinerea，deuteromycotina，which is one of the most important diseases affecting cucumber production．This paper conducted systematic review on the pathogen，occurance regularity，pathogenesis，disease resistance mechanism，disease resistance breeding of cucumber grey mould；and carried out expectation for future work，in order to provide reference for breeding of grey mould-resistant cucumber．
2020 Vol. 1 (7): 15-23 [Abstract] ( 252 ) [HTML 1KB] [ PDF 1533KB] ( 439 )
24 Research Progress on Disinfection and Algae Removing by Nutrient Solution in Soilless Vegetable Culture
CHEN Lei-fu，LIU Zhi-xiong，WU Jin-ping，JIAO Zhen-biao，GUO Feng-ling，QIU zheng-ming，YUAN Wei-ling
Disinfection and algae removing in nutrient solution is a very important technological link in closed vegetable soilless culture system．Combining with the relevant literature at home and abroad，this paper reviewed the species of pathogen and algae in nutrient solution in vegetable soilless cultivation，infection patterns，principle of different sterilization and algae removal technology，development and utilization status．Besides，the paper also put forward new technique ways and scientific issues expecting to provide theoretical basis for subsequent research and popularization and application in future．
2020 Vol. 1 (7): 24-29 [Abstract] ( 241 ) [HTML 1KB] [ PDF 1525KB] ( 509 )
30 Identification of the Mutant Gene yl-2 Conferring the Yellow Leaf Trait in Cucumber
LI Yang-yang，XUE Bing，ZHOU Qian，WANG Bo-wen，CHEN Chen，CHEN Hui-ming，YANG Xue-yong，ZHANG Zhong-hua，LI Zheng，WANG Shen-hao Hot!
Chlorophyll content is closely related to leaf photosynthesis efficiency and crop yield potential，and it is also an important physiological index of crops．Through conducting EMS mutagenesis on seeds of cucumber line HN3，the mutant yl-2 with yellow leaf was screened and obtained．Taking the yellow leaf mutant yl-2 and wild-type cucumber HN3 as parents，this study constructed a BC1F2 seperation population．Genetic analysis showed that this mutant was controlled by a recessive single gene．Through DNA pooling sequencing，we used MutMap strategy to find mutant gene，and obtained 4 satisfactory SNP loci．These 4 SNP loci were distributed in the 13-19 Mb physical interval on cucumber chromosome 6．Only one SNP i. e. SNP17451330 among these 4 SNP was a missense mutation，located on the gene（Csa6G385090），and the other 3 were all located on intergenic variants．By biological information analysis we found that gene Csa6G385090 encoded chlorophyll a oxygenase CAO，and amino acid mutation caused by SNP17451330 was located in the conserved domain of CAO protein．The dCAPS marker designed according to SNP17451330 was co-separated with the leaf color phenotype of the population plants，indicating that Csa6G385090 was most likely to be the candidate gene for the yellow leaf mutant yl-2．
2020 Vol. 1 (7): 30-37 [Abstract] ( 247 ) [HTML 1KB] [ PDF 2836KB] ( 469 )
38 Detection of Cucurbit aphid-borne yellows virus in Southern Xinjiang and System Evolution
Maimaitiaili·Abudunasier，YANG Du，HAN Sheng，Wenqiemu·Abuliezi，Yushanjiang·Maimaiti
In 2019，47 melon leaf samples with typical symptoms of Cucurbit aphid-borne yellows virus（CABYV）were collected from 7 counties/cities in Southern Xinjiang．This study conducted CABYV detection by RT-PCR．One representative sample was selected from each county/city．This study also carried out clone sequencing on specific fragment，and analyzed the nucleotide diversity，protein sequence，and phylogeny．Results showed that 28 leaf samples（Detection ratio was 59.57%）presented CABYV positive．The detection ratioes of Bachu，Akesu，Shache，Jiashi，Shule，Shufu and Luopu were 80.00%，28.57%，83.33%，60.00 %，37.50%，71.42% and 66.67%，respectively．Clone sequencing obtained 707 bp nucleic acid sequence，and the nucleotide diversity value was between 0.012-0.018．All sequences had 2 ORFs（ORF1 and ORF2）．ORF1 encoded 199 amino acid of coat protein（CP）．The sequence similarity of each county/ city was 99.07%．ORF2 encoded 191 amino acid of movement protein（MP）．The sequence similarity of each county/city was 98.80%．The phylogenetic trees constructed on CP and MP sequences base indicated that all sequences obtained from each county/city were all clustered with the Asian branch．Those results demonstrated that CABYV occured commonly in the main melon production areas in Southern Xinjiang，and the nucleotide variation in different regions was very low．CABYV in these regions had close genetic relationship with isolates from China and Japan，while having a farther relationship with European and Taiwan isolates．
2020 Vol. 1 (7): 38-44 [Abstract] ( 230 ) [HTML 1KB] [ PDF 6399KB] ( 345 )
45 Mitigative Effect of Biochar on Cadmium Toxicity and Growth of Pakchoi
XIE Wei-fang，CHENG Ting，LIU Ling-ling，ZHOU Rui-rong，ZHENG Yuan-li，ZHANG Bang-xi
Taking‘Sijixiaobaicai’as test material，this paper studied the effects of biochar addition at different proportions on the mitigation of cadmium（Cd）toxicity in yellow soil and growth of pakchoi．The results showed that adding biochar could obviously increase the pH value of soil contaminated by Cd，significantly decrease the content of available Cd in soil and reduce its bioaccumulation coefficient，thereby relieving the inhibiting effect of Cd on growth of pakchoi．The addition of biochar could also increase the emergence rate，plant height，root length，fresh weight per plant and dry mass per plant of pakchoi；significantly reduced the Cd content in the plant；inhibited Cd being transported from underground to above ground．In general，adding 10% biochar has the best effect．
2020 Vol. 1 (7): 45-50 [Abstract] ( 199 ) [HTML 1KB] [ PDF 1726KB] ( 425 )
51 Effects of Cadmium on Growth of Different Type Melons and Differences of Cadmium Contents in Their Organs
KONG Wei-ping，CHENG Hong，YUE Hong-zhong
In order to find the cadmium（Cd）tolerant melon germplasm resources，this paper took the wild melon，thin-skinned melon and thick-skinned melon as testing material，and adopted pot culture method to study the effects of adding 0，50，100 mg · kg-1 cadmium solution on melons growth and differences of Cd contents in each plant organs．The results showed that with the increasing of Cd concentration，the plant growth vigor was decreased and above ground plant fresh weight and root system fresh weight decreased significantly．The order of Cd contents in melon organs was root＞stem＞leaf＞fruit．Along with the increase of Cd treatment concentration，Cd contents in melon organs：root，stem，leaf and fruit were increased correspondingly．When the Cd concentration was 100 mg · kg-1，the Cd contents in root，stem and leaf of the wild melon were less than those in thin-skinned and thick-skinned melon，but the Cd content in fruit of wild melon was the largest．The Cd residue in soil of 3 melon types were ranged from 35.000-80.000 mg · kg-1，which were far beyond the national Third Grade Soil Standard setting bounds to Cd soil content．The wild melon and thick-skinned melon had good Cd tolerance．The wild melon has strong Cd removal ability．The tested thin-skinned melon was sensitive to Cd．
2020 Vol. 1 (7): 51-55 [Abstract] ( 180 ) [HTML 1KB] [ PDF 1625KB] ( 359 )
56 Determination and Analysis of Fiber Content in Pod Wall of Viburnum Bean Germplasm Resources
QIN Xiang-kai，FENG Guo-jun，LIU Da-jun，LIU Chang，YANG Xiao-xu，YAN Zhi-shan
The content of common bean pod wall fiber（PWF）can directly affect the quality of pod beans．In order to investigate the differences in PWF content of bean germplasm resources，this study adopted pretreatment with α-amylase and neutral detergent method to determine the content of pod wall fibers in 250 common bean germplasms during pod green ripening stage．The results showed that the PWF content of 250 common bean germplasms ranged from 12.5%-38.1%．Among them，the germplasm with the highest PWF content was‘CIAT blue’，and the lowest was‘rolled eyes’．There were bigger variation existed in PWF content of different common bean germplasm resources．Taking SPSS clustering analysis，this study divided 250 copies of kidney bean germplasms into 3 groups according to their PWF contents：10 copies had high content（31.1%-38.1%）；53 copies had medium content（22.3%-29.9%）；187 copies had low content （12.5%-22.2%）．Those common bean germplasms with high PWF content and medium PWF content are not suitable for vegetable pods，while common bean germplasms with low PWF content have better quality and can be used as vegetable pods．
2020 Vol. 1 (7): 56-62 [Abstract] ( 205 ) [HTML 1KB] [ PDF 1875KB] ( 368 )
63 Effect of Fresh-cut and Low-temperature Storage on Quality of Four Vegetables
LI Fang-man，LIU Gen-zhong，ZHOU Guo-lin，ZHANG Ting-yan，ZHANG Yu-yang
By measuring the contents of total sugar，total acid，carotenoids，chlorophyll，and ascorbic acid in 4 kinds of vegetables，including lettuce，potato，pepper，and cowpea，this paper studied the quality changes in fresh vegetables，fresh-cut vegetables and vegetables after low-temperature storage．The results showed that comparing with fresh vegetables the total sugar contents of 4 vegetables were changed significantly after fresh-cut and low-temperature storage．The total sugar content in pepper after fresh-cut and low-temperature storage was extreme significantly increased．While，the total sugar contents in cowpea and potato after fresh-cut and low-temperature storage were significantly or extreme significantly reduced．The total acid contents in lettuce and potato tended to decrease after fresh-cut and low-temperature storage．The sugar-acid ratio of fresh-cut potatoes increased extreme significantly．After fresh-cut and low-temperature storage，the carotenoid contents in lettuce decreased extreme significantly by 32.7%-54.5%．While，that in pepper significantly or extreme significantly increased by 63.5%-112.9%．After low-temperature storage and fresh-cut，the chlorophyll contents in lettuce and pepper decreased extreme significantly by 57.7% and 43.6%，respectively．The contents of total ascorbic acid and reduction ascorbic acid in lettuce and potato after fresh cut and low-temperature storage were significantly or extreme significantly reduced．The contents of total ascorbic acid and reduction ascorbic acid in fresh-cut pepper increased extreme significantly，while those in pepper after fresh-cut and low-temperature storage were decreased extreme significantly．The contents of carotenoids，chlorophyll and ascorbic acid in cowpea after fresh-cut and low-temperature storage did not change significantly．
2020 Vol. 1 (7): 63-69 [Abstract] ( 200 ) [HTML 1KB] [ PDF 1869KB] ( 346 )
70 Studies on Sensitivity of Tomato Botrytis cinerea to Fluazinam in Liaoning Province
CHEN Le，MIAO Ze-yan，SUN Bai-xin，ZHAO Yang，BAI Yuan-jun，DUAN Yu-xi
In order to evaluate the sensitivity of Botrytis cinerea to fluazinam and existed cross-resistance between fluazinam and the other different fungicides，this study collected，separated and purified 206 single spore bacterial strain from tomato production areas of Liaoning Province，and determined their sensitivity baseline to fluazinam by mycelial growth rates method．Results showed that the EC50 values of tomato Botrytis cinerea to fluazinam in Liaoning Province was 0.001 3-0.073 1 μg · mL-1．The mean EC50 value was（0.022 7±0.014 5）μg·mL-1．The sensitivity frequency distribution presented a continuous unimodal curve in accordance with normal distribution．Therefore，EC50 average value（0.022 7±0.014 5）μg · mL-1 could be taken as sensitivity baseline of tomato Botrytis cinerea to fluazinam in Liaoning Province and be used in fields monitoring for resistance to drugs．Thirty-nine B. cinerea isolates，exhibiting different sensitivities to fluazinam selected from 206 bacterial strains were tested on their sensitivities to pyrimethanil，procymidone，fludioxonil，boscalid and iprodione．The results indicated that the inhibitory activities order of these 6 tested fungicides to tomato Botrytis cinerea were fluazinam＞fludioxonil＞boscalid＞iprodione＞procymidone＞pyrimethanil．There were no cross-resistance existing between fluazinam and pyrimethanil，iprodione，procymidone，boscalid，fludioxonil．
2020 Vol. 1 (7): 70-75 [Abstract] ( 201 ) [HTML 1KB] [ PDF 1676KB] ( 350 )
76 Investigation and Analysis of Infl uencing Factors on Post-harvest Quality of Sweet Corn in Beijing
WANG Fu-dong
Taking 9 sweet corn production and sale enterprises in Beijing as respondents，this paper analyzed the factors aff ecting the quality of sweet corn after harvest，and explored the methods for extending sweet corn shelf life harvest．The results showed that the main factors aff ecting the post-harvest quality of sweet corn in Beijing areas were variety，harvest temperature，pre-cooling methods and temperature，post-harvest grading system，package material，transportation mode and storage temperature．Selecting appropriate varieties according to market requirements，carrying out harvest when temperature being low，adopting crushed ice precooling method to quickly reduce temperature，insisting on long-term grading measures，choosing suitable package material，transportation mode and storage temperature can prolonged the shelf life of sweet corn．
2020 Vol. 1 (7): 76-82 [Abstract] ( 211 ) [HTML 1KB] [ PDF 1835KB] ( 467 )
83 A New Cucumber F1 Hybrid —‘Jindong 365’
LIU Nan，DU Sheng-li，HAN Yi-ke，WEI Ai-min，CHEN Zheng-wu，LI Feng-ju
‘Jindong 365’is a new cucumber F1 hybrid developed by crossing excellent DH line‘H08-26’as female parent and inbred line‘DE-2’as male parent．The plant has strong growth vigor．Its fruits primarily set on the main stem．It can grow and set fruit continuously during overwinter cultivation and under low temperature and weak light condition．It has strong successive fruit setting ability and fast fruit swelling speed．The fruit is of high commodity quality，and about 38 cm in length．Its fruit neck is short with moderate spines．The fruit is emerald green in color．Its fruit can keep better glossiness even when grafted with blackseeded pumpkin．Its resistances to downy mildew，powdery mildew，Fusarium wilt，and virus disease are stronger than that of the contrast‘21-10’．Its yielding ability is good．In the middle and late period of overwinter greenhouse cultivation its production is outstanding．The total output can over 300 t · hm-2 under good cultivation and management conditions．It is suitable for overwinter large scale cultivation in solar greenhouse in Tianjin，Hebei，Shandong，Henan，Shaanxi．
2020 Vol. 1 (7): 83-86 [Abstract] ( 217 ) [HTML 1KB] [ PDF 3367KB] ( 414 )
87 A New Hot Pepper F1 Hybrid —‘Xinxiangla No.2’
ZHAO Qun-ying，MIAO Wu，SUN Jia-jie，LIU Yi-long
‘Xinxiangla No.2’is a new medium maturity and small hot pepper F1 hybrid bred by crossing cytoplasm male sterile line of‘9704A’as female parent and inbred line of‘R07172’as male parent．The plant has medium growth vigor．Its plant is 65 cm in height．The first female flower sets on the 12-14th node．The fruits is of sheep-horn shape with a sharp end．Its fruit is 15-18 cm in length，1.9 cm in width，and its flesh is 0.15 cm in thickness．It mainly has 2 heart chambers．It is slightly wrinkled at fruit shoulder．In the early and late growing period，the fruit has good uniformity．Its single fruit weight is about 21.8 g．The immature fruit is green in color and matured one is bright red in color．It tastes medium pungent．The fruit VC content is 1 382 mg · kg-1 and capsaicin content is 0.11%．The yield is about 37.5 t · hm-2．In fields，its resistance to epidemic disease and virus diseases is stronger than that of the contrast‘Xiangla No.2’．It is suitable for planting in greenhouse or open fields in spring-summer in Hunan，Shandong，Guizhou，Shanxi，Sichuan，Henan and Hebei Provinces．
2020 Vol. 1 (7): 87-89 [Abstract] ( 200 ) [HTML 1KB] [ PDF 3386KB] ( 416 )
90 A New Upward Pepper F1 Hybrid —‘Cuijiao No.1’
ZHANG Tian-xiang，XU Xiao-ming，LAI Yun-hong，LAI Yun-huo
‘Cuijiao No.1’is a new upward hot pepper F1 hybrid bred by crossing nuclear-cytoplasmic interaction type male sterility line‘LH0902A’as female parent and inbred line‘CA0802’as male parent．It is of medium maturity．The first flower node sets on the 16-17th node．The plant type is compact with strong growth potential and continuous fruit setting ability．The immature fruit is green in color，and matured one is bright red in color．The fruit is of finger shape，7-8 cm in length，1.8 cm in width，and its flesh is 1.2 mm in thickness．The single fruit weight is about 6.5 g，spicy degree is about 49 000 SHU．The dry matter content is 27.3%．The yield is about 21 t · hm-2．Its resistance to anthrax and virus disease in fields is stronger than that of the contrast‘Fujianwangzhongwang’ ．It is suitable for planting in spring in Fujian areas．
2020 Vol. 1 (7): 90-92 [Abstract] ( 209 ) [HTML 1KB] [ PDF 3421KB] ( 430 )
93 A New Eggplant F1 Hybrid —‘Minqie No.6’
HUANG Jian-du，LIN Hui，GAO Shan，YE Xin-ru，WANG Bin，ZHANG Qian-rong，WEN Qingfang，CHEN Ji-bing
‘Minqie No.6’is a new eggplant F1 hybrid developed by crossing inbred line‘HLB107-2-3-1’as female parent and inbred line‘Yanhou-2-1-1’as male parent．It is of mid-early maturity．It takes 48-53 days from field planting to first harvest．The plant grows vigorousely and is of erect type．Its plant is 105 cm in height，95 cm in width．The first flower sets on about the 11th node．The main stem is purple green in color．Its fruit is of strip shape，35 cm in length，4 cm in width．The peel is shining purple red in color，flesh is white-green in color and sepal is purple green in color．The average single fruit weight is 210 g．It has strong successive fruit setting ability．It can yield over 67.5 t · hm-2．It is resistant to bacterial wilt，Verticillium wilt and Fusarium wilt．It is suitable for facility cultivation in Fujian Province．
2020 Vol. 1 (7): 93-95 [Abstract] ( 176 ) [HTML 1KB] [ PDF 3419KB] ( 444 )
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2020 Vol. 1 (7): 96-100 [Abstract] ( 251 ) [HTML 1KB] [ PDF 2602KB] ( 337 )
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2020 Vol. 1 (7): 101-106 [Abstract] ( 206 ) [HTML 1KB] [ PDF 5281KB] ( 427 )
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2020 Vol. 1 (7): 107-108 [Abstract] ( 223 ) [HTML 1KB] [ PDF 1513KB] ( 321 )
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2020 Vol. 1 (7): 109-110 [Abstract] ( 275 ) [HTML 1KB] [ PDF 1616KB] ( 314 )
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2020 Vol. 1 (7): 111-112 [Abstract] ( 224 ) [HTML 1KB] [ PDF 1754KB] ( 472 )
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