Pumpkin seeds are rich in nutrition value and has higher healthy function.The scale of seedused pumpkin industry in China has expanded year by year.But at present the germplasm resources creation and new variety breeding of seed-used pumpkin are waiting for further systematic study.The paper expounded the evaluation and identifi cation of seed-used pumpkin germplasm resources and genetic diversity research,and genetic localization and regulation of important agronomic characters,including yield,disease resistance, seed coat color,nutrient content of seed-used pumpkin.Meanwhile,the paper prospected the research directions for seed-used pumpkin,so as to provide certain theoretical reference for germplasm creation and breeding of new varieties.
Vegetable industry is developing rapidly in China.The development of vegetable industry in different regions presents different characteristics.This paper established an evaluation index system for Chinese vegetable industry competitiveness,based on the theory of national competitive advantage, and combined with the actual development situation of vegetable industry in China.Then,this paper analyzed the competitiveness of vegetable industry in various regions of China by factor analysis method and system clustering analysis method.The results indicate that ① The development levels of vegetable industry competitiveness in various regions of China are unbalanced.There are bigger differences existed in competitiveness of vegetable industry in different provinces.The provinces with large vegetable production and large agricultural production scale have stronger competitiveness,while those with weaker agriculture foundation and smaller agricultural production scale,possess less competitiveness in vegetable industry. ② The vegetable industry in eastern regions has stronger competitiveness,while western regions has weaker competitiveness.The vegetable industry in eastern regions has good development foundation and many advantages in market requirement and transportation facilities.In western regions,the vegetable production basis is relatively weak,and under the constraints of transportation facilities,the overall competitiveness of vegetable industry is weak.③ The order of provinces with stronger competitiveness in vegetable industry are as following:Shandong > Henan > Jiangsu > Hubei > Hunan > Hebei > Guangdong > Zhejiang > Sichuan.
A heat shock protein gene,naming CmHSP70-5,was isolated from pumpkin cDNA by polymerase chain reaction (PCR) method based on pumpkin genome sequence.Phylogenetic relationship of HSP70-5 and its homology were analyzed using bioinformation method.The expression quantity of CmHSP70-5 under heat stress was studied by real-time PCR.The results showed that CmHSP70-5 gene was 1 953 bp,and its coding CmHSP70-5 protein had the closest relative with Arabidopsis AtHSP70-5.CmHSP70-5 gene had a high expression level in roots than in stems and leaves under normal conditions.Real-time PCR results indicated that under heat stress,the expression of CmHSP70-5 gene in roots,stems and leaves were all up-regulated and could reach the maximum quantity 3 hours after treatment,indicating that the expression of CmHSP70-5 gene was related to heat stress in Cucurbita moschata.
Taking 20 potato advanced generation clones created by foreign germplasm resources as test material,‘Youjin’as susceptible contrast and‘Kexin No.18’ as resistant contrast,this paper evaluated the scab resistance of test material by natural infested field and artificial inoculation methods;analyzed the genetic relationship between test materials using SSR molecular markers;and compared the clustering results of SSR genetic similarity coefficient and classification results of disease resistance of the tested materials, so as to explore the feasibility of using SSR molecular marker for screening resources with scab resistance. Two hundred and seventy-eight bacterial strains with Streptomyces characteristics were separated and purified from soil and tuber,of which 240 from tuber and 38 from soil.One hundred and twenty-seven bacterial strains with pathogenicity were all identified as Streptomyces scabies.There were significant differences in disease resistance among the tested materials,which could be divided into 4 types:high resistant,moderate resistant,moderate susceptible and high susceptible.Correlation between artificial inoculation and resistance identification in natural disease nurseries was highly significant(R2=0.946 7).The resistant type and susceptible type could be roughly distinguished by SSR genetic similarity coefficient clustering,indicating that scab resistance was related to the genetic relationship.
In most areas of Guangxi,there are more rainy days in spring and autumn and the rainfall is higher.This climatic conditions are beneficial to the survival and pathophoresis of Phytophthora infestans and sporangium produced by late blight.This paper conducted resistance test for late blight on 16 large-fruit tomato materials to screen Polymeric multi-resistant materials using field resistance identification and KASP molecular marker technology.The results showed that 10 materials showed high resistant or resistant to late blight in fields;3 materials contained late blight homozygous resistance gene Ph-3;2 materials contained TYLCV homozygous resistance gene Ty-1;4 materials contained TYLCV homozygous resistance gene Ty-3; 10 materials contained Verticillium wilt homozygous resistance gene Ve-2.Besides,3 materials:LF-15,LF- 35,LF-41 had 4 resistant genes at the same time.Among them,LF-35 contained 4 resistant genes all being homozygote.
Taking tomato variety‘stone’as test material,the paper carried out salt stress test on tomato root system by hydroponic device and wiping out main root and lateral root.Four treatments of salt stress concentration were 0(CK),0.1%,0.2% and 0.3%,respectively.The paper studied the growth status and salt tolerance threshold of tomato root system by testing lateral root system activity,dry root weight and root morphological parameters,etc. indexes.The results showed that the growth of lateral roots all accorded with the slow-fast-slow Logistic growth model.Stress with 0.1% salt concentration could delay the development progress of root system.The root system activity,dry root weight and root system morphological parameters were all higher than that of the contrast.Under stress with 0.2% and 0.3% salt concentration,the root system development progress was all ahead of schedule,then the dry root weight and root morphological parameters were all lower than that of the contrast.The growth of root system was inhibited.The injuried main root would stimulate the survival potential of lateral roots conducting compensatory growth.The root morphological parameters had bigger contribution to salt tolerance.Total root length,root superficial area and root volume could be taken as salt tolerance indexes.To sum up,the salt stress environment benificial to lateral root growth is 0.1% salt concentration.The Logistic model can be used to predict the origin,peak and terminal points of root growth,so that effective measures can be taken to regulate the growth of lateral root under salt stress.
Taking 6 varieties as test material,this paper studied on characteristics of floral organ morphology,population florescence,flowering dynamics of a single flower,flowering time of different types of flower,stigma receptivity,pollen viability,outcrossing index and pollination pattern of water caltrop. The results showed that water caltrop flower was solitary and bisexual.A single flower can bloom for 15 h. The population florescence starts from May to October and full bloom stage is July to August.Different types of water caltrop cultivar have different flowering peaks within a day.The period for stigma to receive pollen is mainly within 2 h after the sepal cracked,and the seed set rate can reach over 50%.Pollen viability can be maintained for 7.5 h under natural conditions.The water caltrop is mainly self-pollinated,and also exists partly apomixes and a small quantity of cross-pollination.
Taking annual Aralia elata(Miq.)Seem. as material,this study divided the branches into 1, 2 and 3 levels,according to their lignification and fibrosis degrees;and studied the effects of branch maturity on growth and nutrient contents of young buds,by out-of-season cultivation in solar greenhouse.The results showed that branch maturity could obviously affect the young bud growth and quality.The sprouting rate and single plant bud yield increased significantly with the increase of branch maturity.Besides,the nutrient contents of soluble sugar,soluble protein,VC and polysaccharide,etc. and secondary metabolites including flavonoids and saponins all showed an increasing trend.In conclusion,selecting branches with high maturity and favorable degree of lignification in out-of-season Aralia elata(Miq.)Seem. production could achieve higher economic value.
In order to clear and definite the different pathoogenicity of clubroot from regions all over China,this paper classified the physiological races and pathotypes of clubroot in 20 Chinese cabbages collected from all over the country,using Williamms identification system and 4 Chinese cabbage varieties with different resistances to clubroot.The results of using Williamms identification system showed that 18 Chinese cabbage varieties were belonged to No.4 physilogical race,and 2 were belonged to No.2 physilogical race.The result of identifying 4 Chinese cabbage varieties showed that according to their different pathogenicities,they were divided into 6 types.There were 6 types existing in No.4 physilogical race and 2 types in No.2 physilogical race.The paper deems that the Williams identification system has not been able to precisely divide the physilogical race of Chinese cabbage clubroot pathogen,and it is of important significance to study on screening Chinese cabbage varieties(lines)with different disease resistances and identifying clubroot pathogen pathotypes.
Taking on-site visit and questionnaire method,this paper randomly selected 20 gardens in 5 districts with large areas of vegetable facilities in Beijing;and investigated the current situation of vegetable production,and requirement for light and simplified technology/equipments.The results showed that the average age of workers > 50 years in the vegetable gardens in Beijing took 78.3%.The aging tendency was obvious.There are 14 gardens,where the workers got 80-120 yuan wage per person per day,accounted for 70%,indicating the high labor cost.Over 95% of fertilizer and irrigation quantities were controlled by farmer experience.The management on water and fertilizer was extensive.The light and simplified index was 0.31- 0.57,and that of regional figour was all < 0.5.Demand rate for light and simplified technology and equipments was higher.The paper suggests that Beijing takes the simplifying development road for facility vegetable production;changing the traditional north-south cultivation direction into east-west direction;adopting small machine to instead artificial ridges;developing drip irrigation and applying liquid fertilizer,etc. simple but high efficient technology;so as to push forward the transformation and upgrading of suburb vegetable industry.
‘Heijin No.2’ is a new yellow flesh watermelon F1 hybrid of early maturity developed by crossing inbred line‘2x-313’as female parent and‘ 2x-303’ as male parent.In protected field cultivation and at early spring,its whole growing period is about 90 days and fruit development period is about 30 days.Its plant has stronger growth vigor and is easy to bear fruit.The fruit is of round shape with dark green peel.The peel is about 0.5 cm in thickness.The flesh is bright yellow in color.The center soluble solid content is 12.4%. The average single fruit weight is 1.5-2.5 kg.It can yield over 42 t·hm-2.It is mid-resistant to Fusarium wilt. It is suitable for early facilities cultivation in Shanxi Province and surrounding regions.
‘Tianjiao No.16’ is a new pepper F1 hybrid developed by crossing inbred line‘45-2-1-1-1- 1’as female parent and inbred line‘76-1-1-1-1-1’as male parent.This variety is of medium maturity.Its first flower sets on the 10-12th node.Its fruit is of goat-horn shape.The unripe fruit is dark green in color with wrinkle peel.The fruit is 25.56 cm in length,5.83 cm in width.The single fruit weight is 103.8 g.Its fruit tastes slightly spicy and has good commercial character.It can yield over 75 t·hm-2.Its resistances to virus disease,Phytophthora blight,anthracnose and powdery mildew are stronger than that of the contrast‘ Longjiao No.2’ .It is suitable for cultivation in protected fields and open fields in Gansu Province.
‘Huanaitianyu 782’ is a super sweet corn F1 hybrid developed by crossing inbred line ‘HT-3’ as female parent and ‘XTF’ as male parent.Its plant height is 228 cm,and ear height is 85 cm.Its ear is of tube type.The ear axis is white in color and ear length is 20.5 cm.There are 17 rows per ear,and 39 grains per row.The fresh ear grain is yellow in color and contents of total soluble sugar and reducing sugar are 17.8% and 7.0%,respectively.It is susceptible to smut and stem rot,moderately resistant to large spot,ear rot and dwarf mosaic.Its fresh ear yield is about 15 t·hm-2.Its growth period is about 80 days.It is suitable to be planted in the main producing areas of fresh corn in Shanxi Province.
‘Yujiucong No.1’ is a new variety bred by systematic selection from mutant population of ‘Changbaishanxidasuan’ through 4 generations of selfi ng.The variety has compact plant type and erect leaves folded into “V” shape.It has 6-8 functional leaves during growing stage.This variety is 60-80 cm in plant height,false stem segment is 20-30 cm in length,its stem thickness is 1.5-2.0 cm,and white stem length is less than 5 cm.The single plant weight is 65-90 g.Its resistances to gray mould,loemia,rust and purple blotch are stronger than that of the contrast ‘Lyucui’ and ‘Changbaishanxidasuan’.It can yield about 75 t · hm-2.It is suitable to be cultivated in central Yunnan Province and regions with similar climatic conditions all year round in open fields.