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Current Issue
2015 Vol.1 Issue.8,
Published 2015-08-01
1
DING Hai-Feng, YU Shuan-Cang, WANG De-Xin, JIA Chang-Cai, ZHANG Feng-Lan, XU Yong
Hot!
2015 Vol. 1 (8): 1-7 [
Abstract
] (
285
) [
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1KB] [
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1230KB] (
1003
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8
GAO Qing-Sheng, HU Gui, CHEN Yong-Sheng, GUAN Chun-Song, YANG Ya-Ting
2015 Vol. 1 (8): 8-10 [
Abstract
] (
242
) [
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1141KB] (
720
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11
WANG Ai-Min, WANG Yuan-Quan, ZOU Rui-Chang, RAN Rui-Bi, WANG Xian-Feng, WEI Jian-Hua, DU Ji-Yan
2015 Vol. 1 (8): 11-13 [
Abstract
] (
264
) [
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1KB] [
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1114KB] (
453
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14
Genetic Background Screen of Inter-specific Hybrids between SSR Marker Assisted Chinese Kale and Rapeseed
YU Hai-Long, FANG Zhi-Yuan, YANG Li-Mei, LIU Yu-Mei, ZHUANG Mu, LI Zhan-Sheng, 吕Hong-Hao , ZHANG Yang-Yong
To screen the genetic background and increase the back-cross breeding efficiency of the interspecific hybrids and their back-cross offsprings between Chinese kale（
Brassica oleracea
var.
alboglabra
，CC，2
n
=2
x
=18） and rapeseed（
Brassica napus
，AACC），the F
1
inter-specific hybrids and their BC
1
backcross offsprings between Ogu-CMS Chinese kale and rapaseed were obtained through hand pollination combined with embryo rescue technology. A total of 220 SSR primers were used to detect polymorphisms between the parents，in which 51 pairs of SSR primers showed polymorphism. 33 pairs of primers were selected to analyze the genetic background of 3 F
1
plants and 35 BC
1
plants，with even distribution on 9 chromosomes and stable amplifications．The results of analysis by software NTSYSpc2.11a showed that the similarity coefficients between F1 plants and rapeseed was 0.74，which indicated that the genetic background of F
1
plants was closer to rapeseed than to Chinese kale，while the similarity coefficients between BC
1
plants and Chinese kale varied from 0.26-0.65，with big genetic background differences among BC
1
plants．In all 35 BC
1
plants，the genetic background of individual 14Y1 was the closest to Chinese kale，which was further confirmed by morphological observation．Thus，the individual 14Y1 could be used as donor plant for further backcross.
2015 Vol. 1 (8): 14-21 [
Abstract
] (
415
) [
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1KB] [
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1577KB] (
582
)
22
Studies on Tomato Variety Identification Technology Using SSR Marker
ZHAO Hai-Yan, WU Ming-Sheng, SONG Ge, NIU Qian, TIAN Hui-Fang
Taking 16 tomato hybrid varieties as material，20 pairs of primer suited for tomato variety identification were screened from 200 pairs of SSR primer，and 66 tomato varieties were analyzed. 52 allelic gene variations were identified among these varieties. The allelic gene variation was in the range of 2-5，with an average of 2.6 allelic gene variation each locus. The cultivar identification ability of primer combination was analyzed by genetic clustering method. The results showed that the 66 tomato varieties could be efficiently identified by only 9 pairs of SSR primer. The SSR-DNA fingerprinting constructed with these 9 pairs of primer combination could be used as genetic tag for variety authenticity identification.
2015 Vol. 1 (8): 22-27 [
Abstract
] (
337
) [
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1KB] [
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1256KB] (
779
)
28
Screening and Bio-control Effect Assessment of Trichoderma Strains against 3 Soilborn Vegetable Pathogens
ZHU Ping-Ping, LING Jian, XI Ya-Dong, CHEN Guo-Hua, MAO Zhen-Chuan, YANG Yu-Hong, XIE Bing-Yan
This paper had screened the bio-control resources of 18
Trichoderma
species，including 175 strains，against 3 kinds of soil-born vegetable pathogens as
Fusarium oxysporum
f. sp.
cucumebrium Owen
，
Phytophthora capsici
and
Ralstonia solanacearum
through confront culture experiments and green house potted plant experiments. The results indicated that through confront culture experiments，we have screened out 50
Trichoderma
strains with inhibitation rate as 70.0%-86.3% against
Fusarium oxysporum
f. sp.
cucumebrium Owen
，and 43
Trichoderma
strains with inhibitation rate as 70.4%-88.7% against
Phytophthora capsici
．And the potted plant experiments have screened out 32
Trichoderma
strains with bio-control efficacy of 50.0%-92.7% against
Ralstonia solanacearum
．We further assessed the bio-control efficacy of 13
Trichoderma
strains with antagonistic inhibitation rate higher than75% by potted plant experiment，and found 2
Trichoderma
strains T52（
Trichoderma atroviride
） and 32080（
Trichoderma pleurotum
） with high bio-control efficacy over 60%．
2015 Vol. 1 (8): 28-33 [
Abstract
] (
437
) [
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1KB] [
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1687KB] (
1146
)
34
Studies on Low Temperature Tolerance of Different Pepper Varieties in Seed Germination Stage
ZHU Chen-Xi, MA Yan-Qing, YANG Bo-Zhi, ZHANG Zhu-Qing
In order to establish the identification indexes and temperature conditions of pepper（
Capsicum annuum
L. ）chilling tolerance during seed germination stage，and to discuss the influence of low temperature on germination of different types of pepper varieties，this experiment took different pepper varieties as material，determined their germination percentage，germination potential，germination index and the relative value of these indicators under different temperature conditions. The results illustrated that relative germination index under 18 ℃，and relative germination percentage under 14 ℃ could be used as identification indexes for low temperature tolerance of pepper in seed germination；18 ℃ could be used as pressure condition to screen chilling tolerant varieties. The results also indicated that the seed of early-maturing varieties had better low temperature tolerance than that of medium maturing and medium late maturing varieties； the seeds of var.
acuminatum
Fingern had better low temperature tolerance；and the low temperature tolerance of var.
grossum
sent pepper seed was the weakest.
2015 Vol. 1 (8): 34-38 [
Abstract
] (
353
) [
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1KB] [
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1327KB] (
606
)
39
Effects of Bagging on Color and Brightness of Oriental Melon Peel
CHEN He-Nan, SONG Ting-Yu, ZHANG Xiao-Ming, WU Chun-Yan, CHENG Yan
The value of L、a and b of oriental melon peel were determined by CR-400 colorimeter and the effects of bagging on color and brightness of oriental melon peel by different bag material were studied in this paper．The results showed that in different fruit development periods，bagging had improved the brightness，green and yellow degree of‘ Lyukui’ peel．Bags made of different material had improved the chromatism index of‘ Lyukui’peel．Among them，Kraft paper bag had the best effect on improving the color and brightness of‘ Lyukui’ peel．Bagging had increased the brightness，green and yellow degree of the following 3 varieties fruit peel：‘Lyukui’，‘Chaotianbaijin’ and‘ Feiyue’，but decreased the brightness and color of‘ Balipiaoxiang’ peel．
2015 Vol. 1 (8): 39-42 [
Abstract
] (
384
) [
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1KB] [
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1261KB] (
487
)
43
Effects of Different Anti-seepage Load-carrying Model on Growth and Yield of Sandy Soil Cultured Pepper Plant
ZHAO Yun-Xia, CUI Jing-Ying, XIE Hua, PEI Hong-Xia
Taking‘ Teluomu’ pepper as material，this paper studied the effects of 5 anti-seepage loadcarrying models on the growth and yield of pepper， and the cost of these different 5 anti-seepage load-carrying models. The results showed that anti-seepage load-carrying models had remarkable effects on plant height，stem diameter， dry matter weight， root activity and pepper yield. The yield increased by geotextile line groove processing， ordinary porous brick groove processing， plastic film line groove processing and concrete slab production slots were 17.6%， 15.1%， 8.7%， and 7.4% higher than the CK， respectively. But the yield by concrete slab production slots was reduced 19.5% than the CK. Through comparing the yield of pepper cultured in sandy soil and production cost of 5 anti-seepage load-carrying models， geotextile line groove processing， plastic film line groove processing and ordinary porous brick groove processing were suitable to be extended and applied in greenhouse cultivation in sandy soil， for their low production costs and remarkable yield increasing effects.
2015 Vol. 1 (8): 43-45 [
Abstract
] (
304
) [
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1KB] [
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1127KB] (
425
)
46
Screening Effective Pesticides to Control
Bemisia tabaci
SUN Hai, ZHENG Xiang, WANG Xiao-Qing, LI Yun-Long, HU Bin, WANG Yin, ZHENG Jian-Qiu
5 kinds of new or mixed pesticides〔20% allyl isothiocyanate EW（AITC），99% petroleum oil EC，mixture of 22.4% spirotetramat SC and 20% nitenpyram WG，mixture of 240 g·L
-1
spirodiclofen SC and 20% nitenpyram WG，Bemisia tabaci No.1〕were applied in fields to carry out pesticide effect and phytotoxicity experiments，and to evaluate the fast-acting property，durable effect and security of pesticides control effect．The results indicated that all these 5 pesticides had no injury at the recommended dose．The mixture of 22.4% spirotetramat SC and 20% nitenpyram WG showed better effect on controlling Bemisia tabaci，its durable effect was obvious compared with the contrast 20% nitenpyram，the control efficacy reached 65.6% 3 days after spraying，then to 84.5% 20 days after spraying．This mixed pesticide could be used to control Bemisia tabaci．3 days after taking 99% petroleum oil EC，the control efficacy on Bemisia tabaci reached 53.0%，so it possessed a great potential in controlling Bemisia tabaci in organic and green agriculture production．20% AITC had fast acting effect on Bemisia tabaci，its control efficacy could reach 70.9% 3ays after spraying，but its durable effect was not ideal．Therefore，when Bemisia tabaci was prevailing，AITC should be rapidly applied and combined with other pesticides，which had long-lasting effect to control Bemisia tabaci ．While Bemisia tabaci No.1 and the mixture of 240 g·L
-1
spirodiclofen SC and 20% nitenpyram WG were not suitable for controlling Bemisia tabaci considering a prevention effect，cost and ease of operation and other factors．
2015 Vol. 1 (8): 46-49 [
Abstract
] (
467
) [
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1KB] [
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1217KB] (
465
)
49
Extraction and Antibacterial Activity of Actives Sub
XIA Ri-Zhao, XIE Yan, YIN Wei, ZHANG Ya, LIAO Xiao-Lan
Took inhibition ratio of citrus cankor pathogen as investigation target，lixivite dry powder of Amaranthus leaf blades with ethyl acetate and gained
Amaranthus tricolor
actives．Examined the effect of solidliquid ratio，extraction temperature，and extraction time of
Amaranthus tricolor
actives by orthogonal experiment．The results indicated that different factors had different degree effects on extraction efficiency．Three extraction parameters were ranked in descending order of their effect on extraction of
Amaranthus tricolor actives
as follows solid-liquid ratio ＞ extraction temperature ＞ extraction time．Their optimal levels were 1∶5，30 ℃，24 h，respectively．Under these conditions，the inhibition rate was 73.81%．Ultraviolet radiation，C source（sucrose）and inorganic ion（NaCl） stress had little effects on Amaranthus tricolor actives stability．It posses certain heat tolerance，its inhibition rate was still up to 49.41%，when the temperature was 80 ℃．
2015 Vol. 1 (8): 49-53 [
Abstract
] (
300
) [
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1KB] [
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1452KB] (
470
)
54
A New Cucumber F
1
Hybrid— ‘Zhongnong No. 32’
ZHANG Sheng-Ping, GU Xing-Fang, WANG Ye, MIAO Han
‘Zhongnong No.32’ is a newly bred cucumber F
1
hybrid using inbred line 10889 as maternal parent and 1166 as paternal parent. It grows vigorously with high tolerance to low temperature and weak light.Its fruit is about 35 cm in length and 3.4 cm in diameter with short neck and dark green peel. The fruit has small chamber with light green flesh. There are many white spines and small tuberculates on the fruit peel. It is multiresistant to WMV，ZYMV，PRSV and medium-resistant to CMV，downy mildew，Fusarium wilt and scab. It is early-maturing. It can sustainably bear fruit，and has obvious high yielding potential. Its yield is 150 t·hm
-2
in greenhouse cultivation in winter. It is suitable for solar greenhouse cultivation.
2015 Vol. 1 (8): 54-56 [
Abstract
] (
410
) [
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1KB] [
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1129KB] (
364
)
56
A New Tomato F
1
Hybrid—‘Hongrui No.1’
CHEN Xiao-Jun, WU Duo-Zhi, YANG Guo-Shuai
‘Hongrui No.1’ is a new tomato F
1
Hybrid developed by crossing RZHT257 as female parent and RZHT006 as male parent. This variety is of unlimited growth type. It is of early maturity. It has strong growth vigor. Its leaf is green in color and big in size. The space between inflorescence is 2-3 leaf blades. Its fruit is of round shape. The matured fruit is red in color with hard pulp and has no green shoulder. It has multiple ventricular and good commercial characteristics. The average single fruit weight is 182.7 g. Its shelf life is 8-10 days. The soluble solid content is 5.7%. Its resistance to powdery mildew，leaf mold and early blight is stronger than that of the contrast‘ Zhongshu No. 4’. It is suitable for open and protected field cultivation in Gansu，Shandong，Hebei Provinces. Its average yield is 85.9 t·hm
-2
.
2015 Vol. 1 (8): 56-58 [
Abstract
] (
436
) [
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1KB] [
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1206KB] (
443
)
59
A New Sweet Pepper F
1
Hybird—‘Longjiao No. 13’
ZHANG Hui, XU Jian, HE Wei, LI Yue-Fei, CHEN Li-Xin, LIN Bao-Xiang
‘Longjiao No.13’ is a new sweet pepper F
1
hybrid developed by crossing T8 as female parent and C9 as male parent. Female parent T8 and male parent C9 are stable inbred lines，gained via repeated backcross and selfing of resistant materials with stable inbred lines isolated from Japanese sweet pepper cultivar and French sweet pepper F
1
hybrid，respectively．It is of mid and early maturity．Its plant has strong growth vigor. Its fruit is of square shape with smooth peel. The single fruit weight is over 116.1 g. Its crude fruit is green in color and the ripe fruit is red in color. It has thick flesh. It is resistant to CMV， blight， anthracnose and these stress resistances are strong. It can yield about 75 t·hm
-2
and is suitable for planting in protected and open fields everywhere in Heilongjiang Province．
2015 Vol. 1 (8): 59-61 [
Abstract
] (
378
) [
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1KB] [
PDF
1247KB] (
347
)
62
A New Leaf-edible Mustard F
1
Hybrid— ‘Yongxue No.3’
REN Xi-Liang, WANG Yu-Hong, MENG Qiu-Feng, YANG Ming-Gui, HUANG Yun-Ping
‘Yongxue No.3’ is a new leaf-edible mustard hybrid developed by crossing male sterile line‘ 07-50A’ as female parent and inbred line‘ 07-2-10-1-13-4-1’ as male parent. Its growth period is 105 days from sowing to harvest. The plant is of semi-erect type，with strong growth vigor. Its leaf is light-green in color，with double serrate. The leaf blade is margin incised，glossy and no wax on the surface. The average effective tiller number is 25. The single plant weight is 1.5 kg. Its yield is about 90 t·hm
-2
. It is resistant to virus disease.Its bolting tolerance is medium. It has good processing quality. It is suitable to be planted in the Yangtze River Valley in autumn and winter.
2015 Vol. 1 (8): 62-63 [
Abstract
] (
364
) [
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1KB] [
PDF
1117KB] (
437
)
64
NONG Ye-Bu-Zhong-Zhi-Ye-Guan-Li-Si
2015 Vol. 1 (8): 64-65 [
Abstract
] (
227
) [
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1KB] [
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1097KB] (
336
)
66
ZHANG Yu-Xi
2015 Vol. 1 (8): 66-67 [
Abstract
] (
201
) [
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1KB] [
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1113KB] (
332
)
68
SONG Jia-Wei, WANG Ying-Ying, SHI Yan-Xia, LI Bao-Ju
2015 Vol. 1 (8): 68-69 [
Abstract
] (
240
) [
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1KB] [
PDF
1046KB] (
667
)
70
MA Hai-Yan, ZHANG Jia-Sen, WANG Li, GAO Zhong-Qiang, YU Hong-Jun, Jiang-Wei-Jie
2015 Vol. 1 (8): 70-73 [
Abstract
] (
209
) [
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1KB] [
PDF
1155KB] (
474
)
74
DONG Chun-Juan, ZHANG Zhi-Gang, SHANG Qing-Mao
2015 Vol. 1 (8): 74-77 [
Abstract
] (
292
) [
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1KB] [
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1417KB] (
565
)
78
TANG E, KUANG Bi-Feng, XIAO Chang-Hua, YU Xi-Mao, TAN Mei-Li, DENG Xian-Chao, LIU Xiao-An
2015 Vol. 1 (8): 78-79 [
Abstract
] (
203
) [
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1KB] [
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1643KB] (
482
)
80
JIANG Wei-Feng
2015 Vol. 1 (8): 80-82 [
Abstract
] (
224
) [
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1KB] [
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1175KB] (
390
)
83
WU Yong-Quan, WANG Dian-Hong, MA Jian-Hui, AN Jian-Qiang, SUN Chao, HUANG Zhi-Hui
2015 Vol. 1 (8): 83-85 [
Abstract
] (
234
) [
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1KB] [
PDF
1704KB] (
440
)
86
LIU Chang-Chun, ZHANG Hua-Ren, LIU Li
2015 Vol. 1 (8): 86-89 [
Abstract
] (
247
) [
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1KB] [
PDF
1335KB] (
417
)
89
ZHANG Li, SUN Shi-Xian
2015 Vol. 1 (8): 89-94 [
Abstract
] (
210
) [
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1KB] [
PDF
1730KB] (
381
)
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