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Current Issue
2015 Vol.1 Issue.7,
Published 2015-07-01
1
ZHANG Zhen-He
2015 Vol. 1 (7): 1- [
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314
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6
PENG Liu-Lin, YU Yan-Feng, ZHOU Kai-Hong
2015 Vol. 1 (7): 6- [
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313
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2212
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11
LU Juan, HE Xiao-Xia, WU Yu-Han, WEN Qiu-Lin
2015 Vol. 1 (7): 11- [
Abstract
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317
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550
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15
Potential Analysis on High Efficient Utilization of Waste Vegetable Resources in China
DU Peng-Xiang, HAN , XUE , GAO Jie-Yun, CHEN Qing, LI Yan-Ming
At present it is an urgent task to realize the safe treatment and resource utilization of vegetable waste．This paper analyzes comprehensively the source utilization of vegetable waste from the following aspects：the source of vegetable waste，existing situation，merit and demerit of major treatments，microbial degradation of vegetable waste．Before ending，the paper suggests that composting is the most effective way to achieve fast resource utilization of vegetable waste in China．The paper also prospects the development of resource utilization of vegetable waste.
2015 Vol. 1 (7): 15- [
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470
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21
Correlation Analysis of Primary Umbel and Seed Umbel Characters in Carrot
SONG Hong-Xia, CAO Jing-Jing, WANG Rui-Li, ZHANG Guang-Xing-*
To clear the genetic pattern of primary umbel types in carrot（
Daucus carota
L. var.
sativa
DC.），the umbel type，morphology characteristics and corresponding seed umbel type，seed yield and quality trait of 7 conventional carrot varieties were investigated and analyzed. The results showed that bell umbels accounted for the most，semi-bell was second，and flat umbels took minimum percentage during flowering in these 7 carrot varieties. Total floret number and umbels area of bell type were significantly higher than that of other types. After pollination，bell umbels formed flat seed umbels mostly，while those with flat shape flowers produced mostly closed type of seed umbels. Semi-bell shape always formed flat type and cup type seed umbels. Comparing yield and quality of these 3 types of seed umbels，various indexes of flat type were better than the closed type and cup type. The yield of single flat type seed umbels was the highest，its seed size was the biggest and full，germination percentage and germination energy were maximum，and its EC was minimum.
2015 Vol. 1 (7): 21- [
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412
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1849KB] (
564
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25
Initial Identification and Sequence Analysis of
Melon yellow spot virus
on Cucumber in Shouguang Isolates
QIAO Ning, WANG Xing-Cui, TIAN Su-Bo, LIU Yong-Guang, JIANG Hui-Xia, LI Mei-Qin, ZHU Xiao-Ping
Cucumber leaves infected possibly by
Melon yellow spot virus
（MYSV） were collected from Shouguang， Shandong Province. These samples were detected by RT-PCR. The amplified products were connected to pEASY -T1 Simple cloning vector and then sequencing was conducted. The results showed that the size of target fragment was 505 bp， which was consistent with the expected result. And homology analysis revealed that the MYSV infected cucumber in Shouguang had closer genetic relationship with MYSV-Sanya isolates（ melon -GQ397254） from Sanya，China and MYSV-TW isolates（ watermelon -FJ386391） from Taiwan， China. Their homology could reach 98%.
2015 Vol. 1 (7): 25- [
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377
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1364KB] (
575
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29
Identification of Phytotoxicity and Laccase Properties of Extracellular Macromolecule Substance from
Botrytis cinerea
CHEN Qian, ZHANG Guo-Bin, SHI Min, ZHANG Zhao-Li, YAN Bo, YANG Hong-Yu
The macromolecular substances larger than 14 kD in weight， secreted by
Botryis cinerea
were extracted with dialysis method. These substances were directly inoculated into the leaf blades of tomato and chrysanthemum to detect their phytotoxicity. And the laccase property of these
Botryis cinerea
secreta was identified using tannic acid and RB light blue as the indicator. The results showed that the extracellular macromolecule substances secreted by
B. cinerea
could lead to leaf tissue necrosis， suggesting that they were toxic to the plants. The results also showed that 3 days after inoculation of
B. cinerea
， there appeared chromogenic reaction on tannic acid and RB light blue plate. And 6 days later， the plate’s color changed almost completely，indicating that the extracellular macromolecule substance had the properties of laccase.
2015 Vol. 1 (7): 29- [
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339
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33
Effect of Different Fertilization on Ammonia-oxidizing bacteria and Ammoniaoxidizing archaea in Red Soil Vegetable Field
ZHOU Zhi-Cheng, LUO Kui, TANG Qian-Jun, RONG Xiang-Min, LIU Qiang, HE Fei-Fei
Adopting
amoA
gene clone library construction method，this paper studied the diversity of ammonia-oxidizing bacteria（AOB） and ammonia-oxidizing archaea（ AOA）and correlations between diversity index and urease activity in red soil vegetable field by different fertilization including decomposed organic fertilizer （DNPK），fresh organic fertilizer（FNPK），Nitrogen，Phosphorus and Potassium chemical fertilizer（NPK），Phosphorus and Potassium chemical fertilizer（PK）．The results showed that OTU quantity，Shannon index of AOB library and AOA library represented organic fertilizer（ DNPK and FNPK） were higher than that in chemical fertilizer soil（ NPK and PK）．The dominant AOB groups in FNPK and DNPK treated soil were
Nitrosolobus multiformis
，which represented 88.5% and 68.5%，respectively．The main AOB groups in chemical fertilizer soil（ NPK and PK） were
Nitrosospira
sp．with proportion of 54.8% and 65.5%，respectively．The main AOA groups in red earths were Candidatus Nitrosotalea devanaterra，which occupied 90.9% in DNPK treated soil，84.4% in FNPK treated soil，77.8% in NPK treated soil，and 45.2% in PK treated soil．The urease activity and diversity index in soils treated by DNPK and FNPK were higher than that treated by NPK and PK．There was positive correlation between diversity index of AOA community and urease activity，but there was no marked positive correlation between diversity index of AOB community and urease activity．In conclusion，organic fertilizer was more able to improve the diversity of AOB and AOA communities in red soil of vegetable field than chemical fertilizer．AOA community played more important role in the process of ammoxidation in red soil of vegetable field．
2015 Vol. 1 (7): 33- [
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365
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40
Preliminary Report about Using 4 Chemical Inducers to Control
Cucumber green mottle mosaic virus
Disease
LI Jun-Xiang- , GU Qin-Sheng-*
In order to screen the chemicals，which could induce resistance to CGMMV in bottle gourd 〔Lagenaria siceraria（ Molina） Standl.〕，2,1,3-Benzothiodiazole（ BTH），Brassinolide（ BL），Chitosan Oligosaccharide（ CTS） and Salicylic Acid（ SA），were applied to bottle gourd seedlings to test their induced resistance in this experiment. BTH，BL，CTS and SA were sprayed before the plants were inoculated with CGMMV. Their relative control effects were evaluated.The results showed that 3 days before innoculation，treatments with BTH（ 0.050 g·L
-1
），BL（ 5.0×10
-5
g·L
-1
），CTS（1.0 g·L
-1
），SA（ 0.138 g·L
-1
） could significantly reduce the disease indexes. Among them，5.0×10-5 g · L
-1
had the best control effect，reaching 70.38%. Further studies indicated that after spraying BL，CTS，SA，3 days was the optimum inducing time，while after spraying BTH， the optimum inducing time was 1 day.Those 4 chemicals all showed potentials in inducing bottle gourd to produce resistance to CGMMV. Their differences in resistance were related with inducers and inducing times.
2015 Vol. 1 (7): 40- [
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362
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45
Studies on
Bradysia odoriphaga
Laying Eggs in Different Hosts and Its Phototaxis to LED
YANG Feng-Shan, XU Zhen-Hua, LU Hong-Gang, Li- Chao , Liu-Chun-Guang
In order to achieve capture of live insects， predict occurrence law of field populations， and understand the occurrence dynamic of resistance in field populations， we tested the fecundity and phototaxis of
Bradysia odoriphaga
on 4 families 9 kinds different hosts and 6 different single color LED lights， and then achieved the best hosts and light source. Meanwhile， the efficient capture technology of live insects was obtained by preparing and field applying the collection cage of
Bradysia odoriphaga
. The results showed that the highest egg laying amount of
Bradysia odoriphaga
was gained in Solanum tuberosum with 93.56 eggs， accounting for 45.27% of the total spawning. The largest attracting amount was acquired in green light with 26.75 eggs， accounting for 40.38% of all the attracting amount of Bradysia odoriphaga. In field application， the numbers of insects caught were 700-800 in open field， 300-400 under shed， 100-200 in greenhouse， respectively. Therefore， the capture of live insects was achieved and the existing technology for capturing insects was improved. Thus， a foundation for studying relative relation of
Bradysia odoriphaga
at different regions was established.
2015 Vol. 1 (7): 45- [
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49
Studies on Quickly Testing Pakchoi Seed Vigor by Resazurin Reagent
LIU Chun-Xiang, Li-Yuan-Yuan , Wang- Meng , Hu- Pan
In order to explore simple，rapid and nondestructive method for testing pakchoi〔
Brassica campestris
L. ssp.
chinensis
（L.）Makino var.
communis
Tsen et Lee〕seed vigor，‘Suzhouqing’and‘Shangha iqing’were used as experimental materials under 44 ℃ condition they underwent aging treatment for 0，2，4，6 days to test their seed vigor index and color change of resazurin reagent，absorbancy，and then correlation analysis was conducted．The result showed that the variation trend of these two pakchoi varieties were the same，their germination rate，germination index and vigor index were all dramatically declined along with the increase of aging degree．The color of resazurin reagent was changed from blue to pink then to colorless．The absorbance declined sharply．The seed germination rate，the absorbancy of resazurin reagent，blue hole rate were significantly positive correlated．The correlation coefficients were over 0.96．The seed vigor index，germination index and absorbancy of resazurin reagent were significantly or highly significantly positive correlated．
2015 Vol. 1 (7): 49- [
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294
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53
Response of Chinese Cabbage Seedling’s Root Morphology with Different Tolerance to Potassium Deficiency
LI Hai-Yun, Si-Dong-Xia , 吕Fu-Tang , Zhang-Fu-Jun
Taking Chinese cabbage〔
Brassica campestris
L. ssp.
pekinensis
（Lour）Olsson〕cultivars‘Defeng No.1’（tolerance to low potassium）and‘Fengkang 70’（sensitive to low potassium）as experimental material，studies the response made by dry matter accumulation during seedling stage and root system parameter of Chinese cabbage with different tolerance to potassium deficiency through hydroponics experiment．The results showed that under potassium deficiency（0.3 mmol·L
-1
），the root dry weight，plant dry weight，and root to shoot ratio in‘Fengkang 70’declined significantly，while there were no marked changes in‘Defeng No.1’．On the other hand，the length and surface area of thin roots（Φ ≤ 0.2 mm）and the proportion between thin root surface area and total root system surface area were significantly increased in‘Defeng No.1’，while there were no changes detected in‘Fengkang 70’．All these results indicated that under low potassium stress，the increase of thin root length and surface area of Chinese cabbage cultivar with low potassium tolerance might increase the propotion of thin root length and its surface area，thus strengthen its tolerance to potassium deficiency．
2015 Vol. 1 (7): 53- [
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365
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58
A New Mini Chinese Cabbage F
1
Hybrid —‘Jingchunwa No. 3’
YU Yang-Jun, Zhang-Feng-Lan , ZHANG De-Shuang, ZHAO Xiu-Yun, YU Shuan-Cang, XU Jia-Bing, WANG Wei-Hong, SU Tong-Bing
‘Jingchunwa No. 3’ is a new mini Chinese cabbage hybrid bred by crossing cytoplasmic male sterile line CMS10152 with self imcompatible line 09219. It has flat-topped head and deep yellow inner leaves. It takes about 55 days from transplanting to harvest. It is of small plant type. The plant is erect with dark green outer leaf. Its head shape is cylindrical and flat-topped with 21.9 cm of head height and 10.2 cm of head diameter. Its inner leaves are deep yellow in color. Its single head weight is 0.7 kg. The yield in open field is about 90-105 t·hm
-2
.It is resistant to virus，downy mildew and black rot. Its tolerance to late bolting is strong. Its quality is good. It fits to transplant in high density of 10 000-12 000 plants per 667 m
2
. It has been extended to Beijing， and Hebei， Gansu，Yunnan，Hubei Provinces.
2015 Vol. 1 (7): 58- [
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415
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1121KB] (
495
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61
A New Pakchoi F1 Hybrid—‘Hualyu No.4’
SONG Ju-Hong, YUE Cun-Qi, Zhang-Guo-Cong , Zhang- Chao
‘Hualyu No.4’ is a high quality pakchoi F1 hybrid developed by crossing W-1-8-1 as female parent and S-1-2-9 as male parent. The plant is rather erect， 21 cm in height and 29 cm in width. Its leaf is flat and dark green in color. The petiole is green and 5.3 cm in width，1.4 cm in thickness. The single plant weight is 260 g. It has crisp quality and tastes rich with less fiber. It can yield over 42 t ·hm
-2
in summer and autumn cultivation. Its resistances to virus disease， downy mildew and soft rot are stronger than that of the check‘ Jingguan No.1’ . It has good tolerance to heat. It is suitable for summer and autumn cultivation in northern China.
2015 Vol. 1 (7): 61- [
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304
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1146KB] (
360
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63
A New Hot Pepper F
1
Hybrid —‘Tianjiao No.10’
CHENG Feng-Lin, Yin-Yan-Lan , Liang-Geng-Sheng , Zhang-Zhong-Ping
‘Tianjiao No.10’ is a new mid-early maturing hot pepper F
1
hybrid developed by crossing clampepper inbred 114 as female parent and clam pepper inbred 67 as male parent. The plant has medium growth vigor，and is 83.72 cm in hight and 73.29 cm in width．The single plant can bear 23.5 fruits. The first flower sets at the 7.95 th node．Its fruit is of large claw shape with smooth surface．Its fruit is 28.68 cm in length and 3.14 cm in diameter. The flesh thickness is 0.332 cm . The single fruit weight is 59.25 g．It tastes medium hot and has good commercial character. It is tolerant to low temperature and scant sunlight. It can yield over 60 t·hm
-2
. It is suitable for cultivation both in open and protected fields in Tianshui of Gansu.
2015 Vol. 1 (7): 63- [
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367
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1104KB] (
423
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66
A New Pumpkin F
1
Hybrid—‘Milong’
MA Hai-Long, Yue- Qing , Zhi-Hai-Ying , Miao-Ru-Yi
‘Milong’ is a early-maturing pumpkin F1 hybrid developed by crossing inbred line DP1-121（
Cucurbita maxima
Duch.）as female parent and XT-221（
C. moschata
Duch.）as male parent. The first female flower sets at about the 6th node. It takes 49 days from flowering to harvest. The fruit is blackish green in color，and of oblate shape with shallow ditch. The fruit pulp is thick，and yellow in color. It tastes sweet，floury，and delicate with good flavor. It is tolerant to storage and transportation. The single fruit weight is about 3.0 kg. It yields 67.5 t · hm-2. It is suitable for cultivation in open fields in spring.
2015 Vol. 1 (7): 66- [
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488
) [
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1083KB] (
494
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68
ZHANG Yu-Xi
2015 Vol. 1 (7): 68- [
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223
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354
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70
NONG Ye-Bu-Zhong-Zhi-Ye-Guan-Li-Si
2015 Vol. 1 (7): 70- [
Abstract
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234
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1697KB] (
364
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72
WANG Ying-Ying, SONG Jia-Wei, SHI Yan-Xia, ZHANG Yang, LI Bao-Ju
2015 Vol. 1 (7): 72- [
Abstract
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247
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1015KB] (
663
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75
YANG Mao-Yuan, ZHANG Guo-Sen, LI Bao-Ju, XIE Xue-Wen, YIN Xue-Yun
2015 Vol. 1 (7): 75- [
Abstract
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218
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1119KB] (
607
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77
YAO Da-Xing, XIAO Xu-Lin, LIU Cheng-Zhi, LIU Hou-Cheng, JIANG Wei-Jie, YU Hong-Jun
2015 Vol. 1 (7): 77- [
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318
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494
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80
吕Shen-Bao , LI Nian-Jun, LI Xiao-Xun, WEI Dai-Guo, DONG Wei-Wei
2015 Vol. 1 (7): 80- [
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227
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1125KB] (
568
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82
WEN Lin-Hong, LI Qiong-Fen, LI Gui-Lian, PEI Cheng-Yuan, ZHAO Da-Qin, GUO Jing-Tao, MENG Ping-Hong
2015 Vol. 1 (7): 82- [
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245
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1420KB] (
541
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84
HU Mei-Hua, YANG Feng-Li, SHE Guo-Xing
2015 Vol. 1 (7): 84- [
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252
) [
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1363KB] (
448
)
87
WANG Hao, YANG Ji-De, ZHAN Zong-Bing, ZHANG Xue-Qin, YAN Xiu-Xia, WANG Zi-Yu, WANG Jing, YANG Jie
2015 Vol. 1 (7): 87- [
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241
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421
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89
ZHANG Li, SUN Shi-Xian
2015 Vol. 1 (7): 89- [
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267
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533
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