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  Current Issue
 
2013 Vol.1 Issue.18,Published 2013-09-15
1 General Research Situation on Gummy Stem Blight of Cucurbitacae Vegetables
LIU Shu-Lin, GU Xing-Fang, SHI Yan-Xia, LI Bao-Ju, MIAO Han, WANG Min, WANG Ye, ZHANG Sheng-Ping
Gummy stem blight，caused by Didymella bryoniae，has resulted in an increasing
loss in cucurbitacae vegetables production in China．This paper carries out systematical review on
the pathogenic bacteria，occurrence rule, disease symptom，resistance identification technology，
resistance resources，resistance inheritance regularity，resistance gene molecular biotechnology study
and control method，etc.．Aiming at the problems existing in current research，the paper also puts
forward orientation and focus for future studies.
2013 Vol. 1 (18): 1- [Abstract] ( 714 ) [HTML 1KB] [ PDF 857KB] ( 1068 )
11 Studies on Vertical Transmission Relationship of Vegetable Prices in China
WU Shu, MU Yue-Ying
In recent years，the frequent fluctuation of vegetable prices has aroused concerns
from all walks of life. This paper analyzes the price relations between each link of vegetable industry
chain as before production，among production and post production．Firstly，it analyzes the relation of
long-term equilibrium and short-term changes of prices between different links by the method of cointegration．
Then it further analyzes the price transmission regularity with finite distributed lag model.
The results indicate that the transmission of price indices of agricultural production machinery of the
upstream industry to price indices of vegetable production and consumer price indices in middle-stream
and downstream has a quarter of time delay．While there is no time delay between the price indices of
vegetable production and vegetable consumer price indices in middle-stream and downstream，and the
fluctuation trends of the 2 are in conformity．Overall，the vertical price in vegetable industry chain
transmits rapidly，and the integration degree of vertical market is relatively high．Based on the above
studies，this paper provides relative policy recommendations.
2013 Vol. 1 (18): 11- [Abstract] ( 614 ) [HTML 1KB] [ PDF 1133KB] ( 770 )
19 Cloning and Expression Analysis of Calcium-dependent Protein Kinase （CDPK） Gene in Cucumis sativus L. under Low Nitrogen Stress
HE Hong-Mei, QIN Zhi-Wei, FENG Zhuo, WU Tao, XIN Ming, ZHOU Xiu-Yan
The CDPK was amplified by RT-PCR from a cucumber cultivar‘Jinyan No. 4’ leaves
cDNA under low nitrogen stress．The sequences of primers were designed based on the Csa002986 gene
coding region in the cucumber genome database，which is using Primer Premier 5.0．The gene is 1 584
bp and encoding 527 amino acids．The protein encoded by this gene was calculated to be a stable and
hydrophilic protein，no transmembrane structure and no signal peptide，and with the protein kinase
domain，and EF-hand calcium-binding domain, and serine/threonine protein kinase active site, and
N-myristoylation site，and so on．Expression analysis of CDPK in various parts of cucumber under
different nitrogen concentration treatment showed that CDPK gene in shoot apex reached the highest
expression level，followed by leaves and stems under the nitrogen-free conditions．CDPK gene expression levels in the stem were the highest，followed by shoot tips and leaves under low nitrogen
conditions．CDPK gene in shoot apex were the highest expression level，followed by stems and
leaves under normal and high nitrogen conditions．CDPK gene expression patterns analysis in leaves
showed that the gene is up-regulated under nitrogen-free and low nitrogen conditions，as the nitrogen
concentration decreased，CDPK gene expression is increased and is significantly higher than the normal
level．The expression of CDPK gene under high nitrogen condition is lower than the normal le vels．
2013 Vol. 1 (18): 19- [Abstract] ( 739 ) [HTML 1KB] [ PDF 6534KB] ( 891 )
29 Genetic Analysis of Sourness-associated Traits in Melon （Cucumis melo L.）
ZHU Hui-Qin, FU Qiu-Shi, ZHANG Xin-Ying, 吕Li-Hua , YI Hong-Ping, WANG Huai-Song
Abstract：Six generations of P 1，P2，F1，BC1，BC2 and F2 derived from a crossing between parent
‘60’（acidless） and‘61’（acid） were used to study the hereditary effects of melon fruit in citric
content，titratable acidity（ TA） and pH value by the mixed major gene plus polygene inheritance model
with joint analysis method of multiple generations．The results showed that the genetic model of citric
content was“ one pair of additive-dominant major gene plus additive-dominant-epistasis polygene”．
Heritability of the major gene and polygene in F2 population was 31.06% and 30.48%，respectively．
Genetic model of TA was“ two pairs of additive-dominant-epistasis major gene plus additive-dominantepistasis
polygene”．Major gene heritability of TA in F2 population was 78.06% ，while that of polygene
was 0．The genetic model of pH value was also “one pair of additive-dominant major gene plus additivedominant-
epitasis polygene”，and the major gene heritability in F2 was 84.07%，while that of polygene
was 12.09%．
2013 Vol. 1 (18): 29- [Abstract] ( 649 ) [HTML 1KB] [ PDF 948KB] ( 625 )
35 QTL Analysis of Fruits Related Traits in Melon（Cucumis melo L.）
ZHANG Xue-Jiao, GAO Peng, LUAN Fei-Shi
A recombinant inbred lines（RILs） mapping population of melon（Cucumis melo L.）
comprising 124 F6：7 families was developed from crossing the thick-skin netted melon line WI998
as female parent and thin-skin melon line 3-2-2 as male parent through single-seed descent. This
RILs population was used to construct a linkage map with microsatellite（ simple sequence repeat，
SSR） markers．Screening from 608 SSR markers 150 pairs of primers（24.67%）were detected
to be polymorplic between the 2 parental lines．This map contained 17 linkage groups spanning
1 246.67 cM with a mean marker interval of 9.59 cM．Composite interval mapping method was
used to detect QTLs of traits involved in melon fruit：fruit weight（ Fw），fruit firmness（ Ff），fruit
length（ Fl），flesh thickness（ Ft） and fruit shape index（ Fsi）．Total 15 QTLs of fruit were detected
and mapped on linkage groups 1，4，5，6，8，10，13，14，15，17，respectively. Among them，
8 QTLs had contribution rate over 10%. QTL Ff13.2 located at the 13th linkage group had the largest
contribution rate 26.45%．Marker ECM87 was located in the same site with Fsi8.2，and marker
CM33 was close linkage with Fsi15.1，the genetic distance is 0.6 cM．
2013 Vol. 1 (18): 35- [Abstract] ( 724 ) [HTML 1KB] [ PDF 941KB] ( 789 )
42 Analysis of Melon Germplasms Genetic Diversity by SSR Markers
Wang-Jian-Yu, DENG Zhi-Bin, ZHANG Ai-Ping, WANG Xiao-Wu, XU Min-Jie, WU Jian, ZHANG Hui, WANG Zhi-Peng-　
SSR molecular marker technique was used to assess the DNA fingerprint classification and
genetic relationship of 72 melon germplasm resources and breeding materials. From 20 pairs of SSR primers，
76 polymorphic bands were generated. On average each pair of primers produced 3.8 bands．The clustering
result divided the 72 melon germplasm resources into 7 groups．There were 64 accessions in guoup I，
including Xinjiang local resources and almost all of the self bred melon germplasm resources．This means
that the genetic background of Xinjiang muskmelon germplasm res ources is comparatively narrow.
2013 Vol. 1 (18): 42- [Abstract] ( 721 ) [HTML 1KB] [ PDF 1940KB] ( 883 )
48 Genetic Analysis on Single Fruit Seed Numbers and Seed Coat Color of Bitter Melon
TAN Shu, CHENG Jiao-Wen, CUI Jun-Jie, LI Wei-Peng, HU Kai-Lin
Taking bitter melon inbred line‘ 29’ and‘ Huizhoudading’ as parent，this study
developed 6 generations（P1，P2，F1，B1，B2，F2）from the cross，and analyzed the inheritance of
single fruit seed number and seed coat color，respectively. The results showed that the single fruit seed
numbers of bitter melon was controlled by 2 pairs of equi-dominant major genes plus additive-dominant
polygenes（E-6 model）．The additive effects and dominant effects of the two major genes were equal.
The dominant effect of polygenes is positive and F1 expresses over-parent heterosis．Seed coat color
of bitter melon was controlled by a pair of dominant genes，black is the completely dominant traits to
brown-yellow．
2013 Vol. 1 (18): 48- [Abstract] ( 860 ) [HTML 1KB] [ PDF 890KB] ( 924 )
53 Genetic Diversity Analysis of Amorphophallus Germplasms Using
Morphological Markers
ZHANG Feng-Jie, LIU Hai-Li, JIANG Xue-Kuan, NIU Yi, ZHANG Sheng-Lin
Morphological markers of 96 accessions of amorphophallus were conducted to evaluate
their level of genetic diversity．The results showed that there was remarkable genetic mutation existing
in the morphological traits of the tested amorphophallu germplasms．The average variable coefficient
between various traits of germplasms was 48.1%．Among them，the variable coefficient of bud shape
was the largest（130.6%），and that of lobes color was the smallest（25.6%）．Clustering analysis was
conducted on amorphophallus phenotypic characters．Ninty-six accessions of amorphophallus were
devided into 11 groups taking Pearson correlation coefficient 1 5 as the clustering threshold.
2013 Vol. 1 (18): 53- [Abstract] ( 704 ) [HTML 1KB] [ PDF 1756KB] ( 950 )
61 Studies on Solid Matrix Priming Enhancing Seed Vigor of Cucumber
FANG Ping-Ping, GENG Mu-Fan, LI Xiao-Dong, CHEN Jin-Feng
In order to study on aged seed vigor of cucumber（Cucumis sativus L.），taking 5 years
old cucumber seeds‘ Nanshui No.1’，‘Chaoshi No.1’，‘Nankang No.1’ as experimental materials，
this paper studies the effects of priming by different solid matrix on aged seed vigor of cucumber．The
results showed that the solid matrix priming treatment could improve the membrane permeability，
reduce membrane lipid per-oxidation, increase seed vigor and promote its germination．Generally，
vermiculite and perlite could enhance the germination percentage and germination ratio of cucumber
seeds，reduce the relative electric conductivity，soluble sugar content and malondialdehyde（MDA）
content in seedling leaves，and increase superoxide dismutase（SOD）activity in different extent．
However，there was no significant difference between the coconut husk and the control in germination
characteristics and physiological indexes of aged cucumber seeds．
2013 Vol. 1 (18): 61- [Abstract] ( 670 ) [HTML 1KB] [ PDF 1617KB] ( 627 )
67 Effect of Quantity of Compost Contaminated with Herbicide on Bioavailability of Cucumber
WANG Zhi-Zhong, 吕Xiao , DU Long-Long, LI Yan-Ming, Fredrick C. Michel Jr.
Nowadays chemical weed control is one of the most indispensable methods in
modern agriculture．However，the compost or manure would be polluted by herbicide residual
in the non-target plants．Improper use of the polluted compost may have serious negative effects
on growth of next-stubble crops．In this research，compost contaminated by herbicide was used
in nursery substrate with different proportions to investigate the sensitivity levels of the plants.
Basically we set 3 conditions to test cucumbers on their germin ation rate，internode length，height，
stem diameter，dry weight，leaf number，leaf area，curl and seedling index after sowing in 45
days．These 3 conditions are non-polluted compost condition（C1），Aminopyralid polluted
condition（C2）and Clopyralid polluted condition（C3），and 0，5%，10% and 20% usage of compost are used respectively under these 3 conditions．Results show that the more polluted compost we used，the lower germination rate is. Moreover，symptoms of herbicide damages of
cucumber seedling are found from the delicate plant， small and curl leaves．With the same usage of compost，Aminopyralid tends to have more serious influence on cucumber comparing to Clopyralid，which means the more effective herbicide are used in the compost，the graver influence will be
presented on the following sensitive plants. So we can draw the following conclusions: the compost
contaminated with compound herbicide suppresses the germination rate of cucumber seeds. It can
significantly inhibit the growth of seedlings of cucumber and reduce its seedling index at a lower concentration.
2013 Vol. 1 (18): 67- [Abstract] ( 600 ) [HTML 1KB] [ PDF 1221KB] ( 486 )
72 Resistance Evaluation of Cucumber Varieties Cultivated in China and their Germplasms to Angular Leaf Spot
MA Bai-Zhuang, LI Ying, ZHANG Yan-Ju, QIN Zhi-Wei, ZHOU Xiu-Yan
Taking seedling-inoculation as identification method，this paper identified the
resistance to angular leaf spot of 222 cucumber（Cucumis sativus L.）varieties cultivated in China
and 52 accessions of germplasm. The result indicated that among 222 cucumber cultivars，D07194，
D07207 and D07251 showed high resistance（HR）to angular leaf spot，accounting for 1.4% of all，
and 56 varieties were resistant（R），125 were moderate resistant（MR），30 were susceptive（S），and
8 were high susceptive（HS）．Thirteen accessions from the 52 germplasms，showed high resistance to
cucumber angular leaf spot．They were D0618，C05-056，F02-07.04，C05-36，D0327，C04-02，
D0463，D0435-3，D0328，D0462，D2009-1，129 and‘ Beijin’．No immune germplasm was found.
2013 Vol. 1 (18): 72- [Abstract] ( 596 ) [HTML 1KB] [ PDF 1008KB] ( 480 )
81 Studies on Cucumber Resistance Mechanism to Powdery Mildew Induced by Choline Chloride
CHEN Xi-Jun, SHEN Shi-Wei, CHEN Yin-Feng, ZHANG Qing, ZHANG Jia-Hao, ZHANG Xiao-Ran, HUANG Ben-Li
Choline chloride is a new growth regulator. When the cucumber seedlings were treated
with 10 mmol·L-1 choline chloride，their plant height，root length，dry weight，fresh weight，and
chlorophyll content were remarkably improved．Studies on induce resistance mechanism showed that the
content or activity of β-1，3-glucanase，chitinase，hydroxyproline rich glycoproteins（ HRGP） and
malondialdehyde（MAD）of cucumber seedlings were significantly increased after being treated with
choline chloride．The conidiospores of Sphaerotheca fuliginea were inoculated on the cucumber leaves
treated with choline chloride．The results showed that the germination rate of conidiospores was decline.
The length of germ tubes was shorter and the number of new conidia strings was less than the contrast.
Choline chloride treatment on cucumber plants can delay and all eviate the occurrence of powdery mildew.
2013 Vol. 1 (18): 81- [Abstract] ( 776 ) [HTML 1KB] [ PDF 1179KB] ( 1203 )
88 Identification of Pathogen of Cowpea Ring Spot and Its Biological Characteristics by Liaoning Province
LIU Zhi-Heng, LI Jian-Bing, AN Xin, TANG Shuang-Shuang, ZHENG Chuan, WANG Shi-Wei
The newly discovered cowpea disease in Liaoning Province—cowpea ring spot’s
paroxysm symptom was described and its pathogen was identified in this paper．The pathogen biological
characteristics was also preliminary determined. The results showed that through experiment of Koch’s
rule disease test，identification by morphology and molecular biology technology，and comparison with
sequence of Corynespora cassiicola（Berk and Curt.） in GenBank，we found that the homology was up
to 99%．Therefore，the pathogen of cowpea ring spot was C. cassiicola（Berk and Curt）. The results
of biological characteristics determination showed that cowpea leaf juice was the best medium for mycelia
growth．Mannitol and beef extract were the best carbon and nitrogen sources．The optimum temperature
was 28 ℃，and the optimal pH value was 8-10．The most beneficial medium for conidial production
was cowpea seed juice. The best carbon source was maltose and the best nitrogen source was beef extract．
The optimum temperature was 28 ℃，and the optimal pH value was 9．The optimum temperature for
conidial germination was 28 ℃ and the optimal pH value was 7-10．The lethal temperature for spore
was 60 ℃ for 10 minutes，and that for mycelia was 65 ℃ for 10 mi nutes.
2013 Vol. 1 (18): 88- [Abstract] ( 587 ) [HTML 1KB] [ PDF 2143KB] ( 839 )
96 A New Cabbage F1 Hybrid —‘Qiugan No. 5’
KANG Jun-Gen, DING Yun-Hua, JIAN Yuan-Cai
‘Qiugan No. 5’ is a new cabbage F1 hybrid developed by crossing CMS line CMS021 as
female parent with self-compatible line 95077-7-A1-B6-2-5 as male parent．It is of medium maturity．
It takes about 80 days from transplanting to harvesting．The plant grows vigorously and the opening degree
is 66.7 cm．Its external leaf color is grey and green．The leafy head is green and compact．The head is
flat in shape．The head is 13.6 cm in height，23.6 cm in diameter．The central core is 6.1 cm in length．
Its single head weight is 2-3 kg．It is crispy and tender．It tastes sweet．It is tolerant to head splitting
and heat. It is high resistant to Fusarium wilt，resistant to TuMV and black rot．Generally it can yield
about 75 t·hm-2．It is suitable to be planted in North，South，Northwest and Sout hwest of China．
2013 Vol. 1 (18): 96- [Abstract] ( 729 ) [HTML 1KB] [ PDF 775KB] ( 598 )
99 A New Processing Type White Radish Hybrid —‘Yunluobo No. 1’
Tao-Jing, YUAN Yi, LONG Rong-Hua, LIANG Ming-Tai, ZHONG Li, LI Ping, LI Gui-Hong, LI Shi-Kai-　
According to the demands for producing dried processing white radish，this paper
taking a spring type radish male sterile line introduced from Korea as material and gained a male sterile
line 47A through transit breeding．In 2010，male sterile line 47A was crossed with inbred line 11015.
In 2011，a dried processing type new hybrid white radish named ‘Yunluobo No.1’ was bred．It has long
cylindrical root with green head and 2/3 part is buried in earth．Its root length is 45-50 cm，diameter
7-9 cm．The single root weight is 1.5-2.0 kg． The dry matter content is 8.7%，reducing sugar is 3.68%，
crude fiber is 0.45% and VC is 193 mg·kg-1．It has good commercial and processing character．In
autumn sowing，its growth period is 70-120 days．Its resistance to downy mildew，virus and soft rot
diseases is stronger than‘naibingzongtai’in field investigation．It can yield fresh root about 75-135
t·hm-2．It is suitable to be cultivated in areas with altitude 1 700-2 400 m in Yunnan Province and other region with similar climate condition．
2013 Vol. 1 (18): 99- [Abstract] ( 591 ) [HTML 1KB] [ PDF 788KB] ( 877 )
102 A New Hot Pepper F1 Hybrid —‘Xinjiao No. 23’
GE Ju-Fen, YAN Tong, OU Yang-Wei , GAO Fang-Yu, NUliyanmuguli-·Mu-Ming-Jiang
‘Xinjiao No. 23’ is a medium and early maturing hot pepper F1 hybrid bred by crossing
inbred line 2002053-2-3-1 as female parent with inbred line 2002068-5-2-1 as male parent．
The plant grows vigorously．Its fruit is of long sheep horn shape. Its fruit is 24.4 cm in length，2.98
cm in shoulder diameter，and 1.92 mm in flesh thickness．The average single fruit weight is 42.3 g.
Its fruit has thin peel and crispy pulp. Its average yield is about 60 t·hm-2．Its resistance to virus and
Phytophthora blight is stronger than that of the contrast‘Tianjiao No. 3’in field investigation．It is
suitable to be grown in green house，and under plastic shed for early mature and late autumn cultivation.
It is also suitable for early mature cultivation in open field with plastic sheet cover.
2013 Vol. 1 (18): 102- [Abstract] ( 552 ) [HTML 1KB] [ PDF 814KB] ( 564 )
105 A New Red Glutinous Corn Hybrid —‘Sunuo No. 3’
ZHOU An-Lai, DENG Kun-Peng, ZHOU An-Xin, ZHOU Dong-Qin
‘Sunuo No. 3’ is a new red glutinous corn variety with superior quality and high yield
developed by crossing inbred line W22 as female parent and HN3 as male parent. At Nanjing region in
spring sowing，it takes about 86 days from seedling to harvesting fresh ear，and in summer and autumn
sowing，it will take 65-75 days．The plant has strong growth vigor．The plant type is semi compact.
The leaf numbers are 16-17．Its ears line orderly and have excellent quality．Its grain is red and white
in color．The single ear weight is about 250 g．It can yield 13.5-15.0 t·hm-2．It is suitable to be
cultivated in Shanghai and Hunan Province in spring and autumn.
2013 Vol. 1 (18): 105- [Abstract] ( 565 ) [HTML 1KB] [ PDF 773KB] ( 429 )
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