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  Current Issue
 
2013 Vol.1 Issue.14,Published 2013-07-06
1 Research Progress in Cucurbit Seed Traits Inheritance and Molecular Biology
WANG Min, ZHANG Sheng-Ping, MIAO Han, LIU Shu-Lin, DONG Shao-Yun, WANG Ye, GU Xing-Fang
Seed traits are closely related to plant growth and development，yield and quality
of product organ，and environmental adaptability of plant．It is important to investigate thoroughly
the inheritance and development regulation mechanism of seed traits for improving crop germplasm，
increasing yield and solving the problem of biological energy s ource．In recent years，great progress has
been made in studying Cucurbit seed traits．Studies on inheritance，development regulation mechanism
and molecular biology of seed traits were reviewed in this paper. In addition，problems existing in
current research were discussed and future research orientation was prospected.
2013 Vol. 1 (14): 1- [Abstract] ( 719 ) [HTML 1KB] [ PDF 871KB] ( 1060 )
9 Research Progress of Brassica campestris L. ssp. chinensis（L.） Makino var.
rosularis Tsen et Lee in China
SONG Bo, XU Hai, CHEN Long-Zheng, ZHANG Hui, KUANG Yuan-Yuan, YANG Jing-Hua, YUAN Xi-Han
This paper summarized research progress and achievements made in Brassica campestris
L. ssp. chinensis（L.） Makino var. rosularis Tsen et Lee about its identification，evaluation of
germplasm resources，new variety selection，breeding technology，cultivation technology，physiology
and molecular biology technology．It also analyzed the problems existing in present studies and
prospected the future research tasks for Brassica campestris L. ssp. chinensis（L.）Makino var. rosularis
Tsen et Lee.
2013 Vol. 1 (14): 9- [Abstract] ( 827 ) [HTML 1KB] [ PDF 877KB] ( 889 )
17 Analysis of Influencing Factors on Melon Production and Operation Benefits
—Taking Xinjiang Melon Farmers as Example
ZHOU Zhong-Li, XIA Ying, QU Song
Taking 181 households of Xinjiang melon farmers as examples，on the basis of
investigation data，this paper carried out descriptive statistical analysis on the factors influencing
production and operating statusquo of melon. The paper adopted semilog multivariable linear regression
method, and systematically analyzed the factors influencing melon production and operation benefits
in Xinjiang，and the correlation between planting scale and benefit．The results indicate that the
benefits are influenced by planting pattern，production mode，planting scale and region selection，etc．
Accordingly，the paper puts forward suggestions in detail to improve the melon production and operation
benefits in Xinjiang.
2013 Vol. 1 (14): 17- [Abstract] ( 665 ) [HTML 1KB] [ PDF 909KB] ( 762 )
25 Construction of Watermelon Genetic Map and Fruit Shape Index QTL
Analysis
WANG Zhao-Ji, GAO Peng, LUAN Fei-Shi, LIU Shi
Taking round shape watermelon line‘ Huayuanmuben’ as female parent and oval
shape line‘LSW-177’as male parent，we crossed them and obtained F2 population constructed with
180 single plants．We measured the length and width of the ripened watermelon〔Citrullus lanatus
（Thunb.）Matsum. et Nakai〕，obtained fruit shape index from the ratio between fruit length and width，
constructed the genetic map and analyzed the QTLs of relevant characteristics．The constructed genetic
map contains 45 SSR-markers belonging to 13 linkage groups．The covering genome length was 547.3
cM．The average distance between markers was 12.44 cM．The one QTL closely associated with fruit
shape index was located in the 3rd linkage group between MCPI-12 and CMN05_69．This QTL was
named as Fsi3.1．Its contribution rate was 2.8%，and its additive effect was negative，which showed
synergia additive effect in reducing fruit shape index.
2013 Vol. 1 (14): 25- [Abstract] ( 922 ) [HTML 1KB] [ PDF 904KB] ( 875 )
31 Purity Identification and SNP Site Obtain of Chinese Cabbage Hybrids Using
Multiplex EST-SSR Marker
ZHAO Xin, WANG Yong, LAN Qing-Kuo, HE Chang-Zheng, CHEN Rui, LI 欧Jing, LIU Na
In this research，10 Chinese cabbage〔Brassica campestris L. ssp. pekinensis（Lour）
Olsson〕hybrids and their parents were taken as study objects．Based on the EST sequences from
NCBI genome data base，a SNP site and multiplex EST- SSR sites，which could be used to identify the
purity of Chinese cabbage hybrids，were screened by SSR marker and high resolution melting（HRM）
technology．According to the requirement of seed purity identification by high throughput molecular
technology，the key techniques such as seedling culture condition of single seed，extraction status of
single grain plant, rapid DNA extraction method and establishment of multiplex PCR system were groped
and optimized．Results showed that the single germinating seed after 48 h could gain DNA with higher
quality by the improved CTAB method in 3 h. Meanwhile，plant leaves reached seeding stage after 7
d，could complete DNA extraction by chexe-100 method within 40 min．The SNP site obtained by screening could be used to indentify the purity of 6 Chinese cabbage hybrids，and the multiplex ESTSSR
locus could be used to indentify the purity of 10 Chinese c abbage hybrids．
2013 Vol. 1 (14): 31- [Abstract] ( 761 ) [HTML 1KB] [ PDF 1594KB] ( 825 )
39 Prokaryotic Expression Vector Construction of Plutella xylostella Cadherin
Fragments and Optimization of Its Conditions
ZHENG Xiao-Xu, YANG Feng-Shan, ZHU Xun, ZHANG You-Jun
In this study，we constructed a recombinant of Plutella xylostella cadherin gene
fragment．In order to enhance its expression in Escherichia coli，we studied the effect of time，
temperature and IPTG concentration on fusion protein expression and its solubility．The results showed
that the fusion protein gave rise to the maximum expression when host cells were cultured in LB at 37 ℃
for 1.5 h and then induced by 0.1 mmol·L-1 IPTG at 25℃ 180 r·min-1 for 36 h．And the temperature
change had little impact on the solubility of fusion proteins. SDS-PAGE test also indicated that the
molecular weight of fusion proteins was the same as expected，wh ich is about 16 kDa.
2013 Vol. 1 (14): 39- [Abstract] ( 686 ) [HTML 1KB] [ PDF 1200KB] ( 1091 )
46 Analysis of Genetic Diversity Morphological Marker in Eggplant Germplasm
Resources
ZHANG Nian, WANG Zhi-Min, YU Xiao-Hu, DING Ze-Qin, TANG Qing-Lin, TIAN Shi-Bing, WANG Yong-Qing
This study evaluated the level of genetic diversity of eggplant（Solanum melongena
L.） germplasm resources through making morphological markers on 76 copies of eggplant germplasm
resources. The results showed that the morphological traits of the tested eggplant germplasm showed
genetic differences. The average variable coefficient between each germplasm traits was 54.83%，of
which the largest variable coefficient was leaf spines，493.3%，the least variable coefficient was fruit
curvature，10.9%. Clustering analysis was carried out on eggplant phenotypic character，76 eggplant
materials were divided into 3 groups taking Pearson correlation coefficient 23 as clustering threshold，if
taking Pearson correlation coefficient 19.5 as clustering threshold，76 eggplant materials were divided
into 7 sub-groups.
Key words：Eggplant；
2013 Vol. 1 (14): 46- [Abstract] ( 806 ) [HTML 1KB] [ PDF 878KB] ( 692 )
53 Obtain and Chromosome Fragments Analysis of BC1 Populations between Lycopersicon
esculentum Mill. and L. peruvianum
ZHAO 　He, WANG Xiao-Xuan, DU Yong-Chen, GAO Jian-Chang, GUO Yan-Mei
Abstract：In this paper，cultivated tomato Lycopersicon esculentum Mill. 1052 was used as female
parent and wild tomato L. peruvianum LA3858 was used as male parent．One F1 hybrid was obtained
by embryo rescuing method．The hybrid was backcrossed with Lycopersicon esculentum Mill. 1052 to
generate BC1 progeny．And 6 single plants of backcross F1 species hybrids were obtained. Moreover，129
primers of CAPS，SSR and InDel on 12 chromosomes were used to analyze chromosome fragments of BC1．
The results showed that these 6 BC 1 plants contained the whole genome of L. peruvianum LA3858．
2013 Vol. 1 (14): 53- [Abstract] ( 711 ) [HTML 1KB] [ PDF 1396KB] ( 786 )
59 Studies on Internal Physiological and Biochemical Changes of Xantha Mutant
in Melon Leaves
SHAO Qin, YU Ze-Yuan, LI Xing-Guo, LI Wei, GAO Yan-Juan
Xantha mutant 9388-1，a spontaneous mutant，was screened out from a normal green
leaf melon（Cucumis melo L.） variety‘ Baishami No.1’．Its contents of photosynthetic pigment and Chl
synthesis precursors，chlorophyll fluorescence kinetic parameters and ultra-structure of chloroplasts
were studied with mutant parent‘ Baishami No.1’ as contrast．The results showed that photosynthetic
pigment content in mutant leaves was significantly lower than that in‘ Baishami No.1’，while the ratio
between chlorophyll a and chlorophyll b was significantly higher than in‘ Baishami No.1’．Chlorophyll
biosynthesis was hampered at conversion from prophobilinogen（PBG） into uroporphyrinogen
（Urogen Ⅲ）．Compared with chlorophyll fluorescence kinetic parameters of‘Baishami No.1’，F0，
Pn，ФPS Ⅱ and ETR were significantly lower，while qN and NPQ were notably higher than that of
‘Baishami No.1’．There were no significant differences in Fm，FV/Fm and qP ．Under transmission
electron microscopy，9388-1 leaves were found with underdeveloped chloroplast，containing aberrant
thylakiods，fewer graum and starch grains than that of‘Baishami No.1’．Grana lamellae were deranged
with a line shape and less stacked，but grana lamellae was crowed together in normal one．These
results indicated that the biosynthesis chlorophyll was hampered，leading to aberrant chloroplast and the
photosynthetic pigments content changed，and then affected their stability．The mutant kept a higher
heat dissipation capacity，which could prevent photosynthetic apparatus from damaged by excessive
light energy and maintain the normal growth of plant.
2013 Vol. 1 (14): 59- [Abstract] ( 679 ) [HTML 1KB] [ PDF 1963KB] ( 766 )
66 Effect of Different Bag Material on Growth，Yield and Quality of Cucumber
Cultivated in Bag
MA Yan, LI Fang, SUN Zhou-Ping, SHEN Xiang-Jun, MA Jian
Taking cucumber（Cucumis sativus L.） cultivar‘ Jinyou 30’as testing material，
corn stalk∶cow dung∶slag=3V∶1V∶1V as cultivation medium，taken permeable woven bag，
impermeable woven bag，nonwoven and double color film as 4 treatment bag materials，we investigated
different bag materials effects on growth，xylem sap，fruit yield and quality of cucumber cultivated
in bags in greenhouse． The results showed that after transplanting 40 days and 55 days， the height of
cucumber cultivated in impermeable bags were significantly higher than that of other 3 treatments．The
fresh weight of above-ground and dry weight of under-ground，and the nutrient content in cucumber
xylem sap was also significantly higher than the other 3 treatments．Moreover，the fruit contents of
soluble protein and VC were also higher than that of the other treatments．The results indicated that the
impermeable woven bag was the optimum bag material for cucumber cultivated in bag．
2013 Vol. 1 (14): 66- [Abstract] ( 728 ) [HTML 1KB] [ PDF 899KB] ( 722 )
72 Effect of Straw Compound Substrate on Tomato Seedling Culture
SONG Zhi-Gang, YU Hong-Jun, JIANG Wei-Jie, ZHANG Ye, YANG Xue-Yong
Taking‘Zhongza 106’tomato（Lycopersicon esculentum Mill.）as experiment
material，and traditional peat and vermiculite media as contrast，this experiment studied on the
physical and chemical properties and effect of tomato seedling culture with different mixing proportion
substrates of decomposed straw，rice husk and sand．The results indicated that the mixing proportion
substrates of straw could meet the requirement of seedling media and make the quality of tomato seedling
better than that of the traditional peat and vermiculite substrates．Among all treatments，T1（100%
straw） and T2（straw：rice husk=75V∶25V） compound substrates could culture tomato seedling with
the best quality．Its physiological index was notably superior t o the other treatments and contrast.
2013 Vol. 1 (14): 72- [Abstract] ( 675 ) [HTML 1KB] [ PDF 998KB] ( 744 )
78 Reproductive Type Confirmation of Taraxacum coreanum Nakai
ZHANG Jian, WU Zhi-Gang, ZHAO 　Xin, MA Ming, NING Wei
Counting root tip chromosome numbers，artificial pollination experiments and flow
cytometry，etc. were used to confirm that Taraxacum coreanum Nakai was tetraploid. Artificial
pollination experiments showed that Taraxacum coreanum reproductive type was facultative apomixes，
and apomixes rate was around 50%．The result of testing by flow cytometer showed that the ratio of seed
cell ploidy level was 2C∶3C∶4C．The reproductive type of tetraploid T. coreanum was facultative
apomixes with independent endosperm development．
2013 Vol. 1 (14): 78- [Abstract] ( 656 ) [HTML 1KB] [ PDF 1109KB] ( 686 )
81 Status of Pesticide Residue and Regulation Analysis in Vegetables Produced in
Inner Mongolia during the Past Decade
ZHANG Fu-Jin, ZHANG Xin-Xin, LI Guo-Yin, FENG Xiao-Hui, REN Chao, LUO Hong, GAO Tian-Yun
Mathematical statistic analysis was carried out on 6 800 samples of veggies，fruits，
beans，nuts and seeds produced in Inner Mongolia. The results showed that the dominated vegetables
in Inner Mongolia were green leaf vegetables，potato family vegetables and melons，and their yields accounted for 70% of the total vegetables production. Since 2003，with the rapid increase of
detected vegetable numbers，the status of vegetable quality safety had entered into a new stage of
sustainable steady after a rapid decline or periodic fluctuation，and the differences in safety levels
were obvious，of which the range of exceeding standard rate of pesticide residues in eggplants，melons
and beans（about 55% of the total vegetables）was under 2%，with a mean of 1.1%，1.6% and
3.1%，respectively. They belonged to stable type. The exceeding standard rate of pesticide residues
in green leaf vegetables and Chinese cabbage（about 30% of total vegetables） presented a decreasing
trend year by year，wondering in the range of 7%-10%．They belonged to risk main body type．The
time period of exceeding standard of pesticide residues in root vegetables and cabbage was under
predictable，and in some years its exceeding standard rate was over 5%（amplitude variation was
over 15 percentage points）．They belonged to random risk type．The kinds of pesticides，which
exceeding standard rate in vegetables were relatively intensive，presented variance in different
vegetable species．70% of the pesticides belonged to intermittent over-limits and the probability was
below 5%．About 20% tradition pesticides often exceed standard and their probabilities were over
30%．The exceeding standard of organophosphorus and carbamates pesticides in vegetables presented a
decreasing tend，while the risk of some new type pesticides containing nitrogen atoms and high effective
pyrethroid insecticide showed an increasing tendency.
2013 Vol. 1 (14): 81- [Abstract] ( 693 ) [HTML 1KB] [ PDF 986KB] ( 579 )
88 Studies on Effects of Controlling Watermelon Fusarium Wilt by Grafting and
Biological Control
SUN Xing-Xiang, LIN Hong-Mei, HE Chun-Ping, NI Wei
Fusarium wilt is an important factor causing clogging in continuous cultivation of
watermelon〔Citrullus lanatus（Thunb.）Matsum. et Nakai〕．In production，we generally adopt crop
rotation，grafting，biological control and medical treatment to prevent this disease．This experiment
studied on many controlling methods，such as grafting on different stock varieties，using biological
pesticides and biological fertilizer，etc．at different plot of land during the period of watermelon
continuous cultivation．The results indicated that the effect of biological control was not obvious，if
the continuous cultivation period was long，and watermelon was planted in the fields where Fusarium
wilt occurred seriously. Biological control could only play an inhabiting role in dome extent，while the
grafted seedling had remarkable effect，the control effect is 100.0%．If the continuous cultivation
period was short，and the plot had slightly suffered from Fusarium wilt，it was efficient to apply BIO
biological fertilizer or grafting.
2013 Vol. 1 (14): 88- [Abstract] ( 679 ) [HTML 1KB] [ PDF 806KB] ( 551 )
92 Control Effect of Pepper Bacterial Wilt and Stability Test of Mixed Pesticides
HE Run-Yun, ZHOU Ai-Zhu, CHEN Xiao-Zhen, LI Hai-Ding
Taking 20% Bismerthiazol WP，72% Streptomycin WP，77% Kocide WP a s experiment
materials，we found that 20% Bismerthiazol WP mixed with 72% Streptomycin WP could prevent and
cure pepper bacterial wilt．The average control effect could reach 96.51% with better stability，which is
worthwhile to extend．
2013 Vol. 1 (14): 92- [Abstract] ( 595 ) [HTML 1KB] [ PDF 803KB] ( 715 )
95 A New Cherry Tomato F1 Hybrid ─‘Hajiao’
CHEN Qing-Qi, MEN Wan-Jie, LEI 　Na
‘Hajiao’ is a new cherry tomato F1 hybrid developed by crossing inbred lines 5-121212
as female parent and 8-322311 as male parent．This variety belongs to semi-unlimited growth type
．The plant is dark green in color with single raceme and of common leaf shape．It has yellow flower，
pink and long round fruit with sharp．The single fruit weight is 15-20 g．Its growing period is about 110
days．Its fruit tastes sweet and sour．It is mid-resistant to leaf mold and virus disease，and is suitable
for protected cultivation. It can produce about 52.5 t·hm -2.
2013 Vol. 1 (14): 95- [Abstract] ( 616 ) [HTML 1KB] [ PDF 795KB] ( 542 )
97 A New Potato Variety —‘Longshu No.11’
LI Gao-Feng, WEN Guo-Hong, LI Jian-Wu, WANG Yi-Hang, ZHANG Rong
Longshu No.11’ is a new potato variety selected from L9712-2（92-29-81×93-1-204）
as female parent with‘ Yuanza 22’ as male parent．It is of late maturity. Its growing period is about
124 days. Its tuber is round with yellow micro-textured peel，yellow flesh and medium shallow eyes．
The average content of dry matter，starch，crude protein，VC and reducing sugar is 23.7%，18.68%，
3.16%，144.2 mg·kg-1 and 0.162%，respectively．It has higher resistance to late bligh t than‘ Longshu
No.5’and has better resistance to leaf roll virus in field．It is sui table for open field cultivation in highaltitude，
cold and wet，and semi-arid region. It can yield about 20.25 t·hm-2.
2013 Vol. 1 (14): 97- [Abstract] ( 577 ) [HTML 1KB] [ PDF 806KB] ( 628 )
100 A New Variety of Yellow Flesh Seedless Watermelon —‘Shenyu’
ZHOU Quan, ZHU Bie-Fang, MA Lu-Ping, YI Xue-Sai, ZHOU Jian, PENG Qi-Feng
Shenyu’ is a new watermelon variety with small and medium size fruit，yellow flesh，
and seedless．The whole growth period of this variety is about 100 days．It takes about 30 days from
female flower bloom to fruit mature．The plant grows in medium and strong vigor．Cauline leaf is in
medium level．It has strong resistance to disease and stress. The fruit is of short oval shape with dark
green peel．The thickness of fruit peel is about 1.0 cm．The fruit has bright yellow flesh，tastes crispy
and refreshing with less fiber. Its seedless performance is good．The central soluble solid content is
11.5%-13.5%．Edge soluble solid content is 7.5%．Its single fruit weight is 2-4 kg．It can normally
yield about 27-30 t·hm -2.
2013 Vol. 1 (14): 100- [Abstract] ( 649 ) [HTML 1KB] [ PDF 809KB] ( 640 )
103 A New Sweet and Waxy Corn Variety—‘Gantiannuo No. 3’
ZHANG Li-Rong, HUANG You-Cheng, HAO 　Kai, ZHOU Ji-Bing, CHEN Xiao-Jun, WU Guo-Jing
‘Gantiannuo No.3’ was a new colored waxy corn bred by crossing double heterozygote
（J38×J40）as female parent and sweet waxy inbred line‘Tiannuo 7’as male parent. J38 and J40 were
waxy corn with white seed coat and purple seed coat，respectively．The sweet waxy inbred line‘ Tiannuo
7’carries the homozyogous super sweet and waxy genes．‘Gantiannuo No.3’ is a new table corn．Its
plant height is 232 cm，with 105 cm ear height. It is resistant to lodging with good stay-green ability and
wide adaptation. Its edable quality is superior．The ear shape is mainly of cone type. The fresh ear can
yield about 16.5 t·hm-2．It takes about 106 days from planting to harvesting．‘Gantiannuo No.3’is
suitable to be planted at spring maize area in Northwest China.
2013 Vol. 1 (14): 103- [Abstract] ( 673 ) [HTML 1KB] [ PDF 858KB] ( 654 )
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