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Current Issue
2010 Vol.1 Issue.10,
Published 2010-05-15
1
Research Progress on Disease Resistance Breeding of Eggplant
WEI Xiao-san;CAO Bi-hao*;LEI Jian-jun;CHEN Guo-ju;CHEN Qing-hua
This paper reviews the recent research progress made in eggplant（
Solanum
melongena
L.）disease resistance breeding, including resistance to bacterial wilt, Verticillium wilt and Fusarium wilt. It also analyzes the existing problems and prospects the future research orientation.
2010 Vol. 1 (10): 1-8 [
Abstract
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1463
) [
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1KB] [
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349KB] (
1971
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9
Research Progress on Tissue Culture of Ginger（
Zingiber officinale
Rosc.）
LIU Zhen-wei;LI Qing-zhi;SHI Xiu-juan
This paper expounds the internal and external research progress made in ginger（
Zingiber officinale
Rosc.）tissue culture, including culture of its organ, cell, protoplast and the plant regeneration. The paper also analyzes key issues existing in ginger tissue culture.
2010 Vol. 1 (10): 9-15 [
Abstract
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1389
) [
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314KB] (
1252
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16
Biological Identification of Low Temperature Tolerance Gene Transform of Cucumber and Its Progeny
LU Shu-wen;LIU Wen-ping;LI Zhu-gang;NAN Xiang-ri;LIU Jian-xin;QIN Zhi-wei
Low temperature tolerance gene
BnCS
was transformed into cucumber（
Cucumis
sativus
L.）plants. Biological characteristics of transgenic plant（T0）and its progeny（T1）were biologically identified. Under low temperature stress at 13 ℃, the germination potential, germination percentage and radicle length of transgenic T0 seed were significantly higher than the CK（cultivar yd-36）. The percentage of electrolyte leakage and MDA content（T1）were significantly lower than the CK. While the increment growth of plant height, stem width and leaf area were higher than the CK. This indicated that low temperature tolerance of transgenic plants was obviously higher than that of the non-transgenic plants.
2010 Vol. 1 (10): 16-19 [
Abstract
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1112
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275KB] (
658
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20
Studies on Isolation of Resistance Related-genes to Black Rot by Suppression Subtractive Hybridization in
Brassica oleracea
ZHU Yan;WANG Chao*
In this research, suppression subtractive hybridization（SSH）was adopted to construct a mixed SSH library by inducing black rot to disease resistant cabbage（
Brassica
oleracea
L.）variety A21 for 6h, 8h, 12h and 24h. Through reverse northern screening, 87 ESTs with high quality were obtained. After conducting comparative analysis with BLAST data bank in NCBI, 83 of the ESTs were found to have homology by different degrees with known protein or genes.
2010 Vol. 1 (10): 20-24 [
Abstract
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989
) [
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1KB] [
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635KB] (
633
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25
Physiological Response to High Temperature Stress in Radish Seedlings with Different Heat Tolerance
WU Bin;JIANG Qiu-wei;GU Ting-ting;ZHAO Mei;LIU Li-wang*
Under high temperature stress（38 ℃）, the variation of MDA, soluble protein, soluble sugar, free proline contents in radish（
Raphanus sativus
L.）seedling with different heat resistance were analyzed. The soluble protein content was higher in heat resistant inbred lines than that in heat-sensitive ones, while the content of soluble sugar, free proline and MDA exhibited no significant difference among inbred lines. Under the treatments of 38 ℃ for 24 h, 48 h and 72 h, the contents of soluble protein, soluble sugar and free proline in heat resistant inbred lines were significantly higher than those in heat-sensitive ones, while the MDA content was significantly lower in heat resistant materials. Under the treatment of 38 ℃ for 24 h, the contents of all the 4 indexes were significantly increased. The soluble protein content declined continuously for 48 h and 72 h, but it declined much slowly in heat resistant inbred lines. As compared with the treatment of 38 ℃ for 24 h, the contents of soluble sugar, free proline and MDA were significantly higher for 48 h in heat-sensitive materials, while in heat resistant materials, there were no significant increases for 48 h and 72 h. It was suggested that the variation of these 4 indexes was related with the heat resistance and could be used for evaluation of heat resistance in radish seedlings.
2010 Vol. 1 (10): 25-28 [
Abstract
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1234
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1KB] [
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266KB] (
695
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29
Soil Pb and Cd Accumulative Characteristics in Swamp Cabbage（
Ipomoea aquatica
Forsk.）and Their Environmental Critical Values in Production Area for Food Security
WU Qi;YANG Fei;JI Hui;ZHANG Wei-jian;*
Plumbum（Pb）, cadmium（Cd）accumulative characteristics in swamp cabbage （
Ipomoea aquatica
Forsk.）under 2 types of soils（paddy and alluvial soils）were studied by potted experiment, and their environmental critical values for food security in its production area were also discussed. The results showed that: the accumulative amounts of Pb and Cd in swamp cabbage were correlated with added amount of Pb and Cd in soil. The accumulative amounts in stem were much higher than those in leaf. Under paddy and alluvial soil comdition, the average levels of Pb in stem were 3.86 and 2.02 times of those in leaf, and the average levels of Cd in stem were 3.58 and 4.73 times of those in leaf, respectively. Under same treatment level, Pb and Cd in paddy soil were easily accumulated by crop than in alluvial soil. In paddy soil, the Pb and Cd contents in leaf of swamp cabbage were 4.88 and 2.56 times of those in alluvial soil, and Cd contents were 3.04 and 4.02 times of those in alluvial soil, respectively. according to national standards for maximum contamination level in food（GB 2762—2005）, the environmental critical values of soil Pb in paddy field was 57.83 mg·kg-1, and the environmental critical values of soil Pb and Cd in alluvial field were 77.02 mg·kg-1 and 0.47 mg·kg-1, respectively for safe swamp cabbage growing. These critical values were both significantly higher than those in the national standards for soil security（HJ 332—2006）.
2010 Vol. 1 (10): 29-34 [
Abstract
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1338
) [
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1KB] [
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363KB] (
880
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35
Several Factors Affecting Embryoid Induction from Isolated Microspore in Kohlrabi（
Brassica oleracea
L. var.
caulorapa
DC.）
WANG Chao-nan;WEN Feng-ying;LIU Xiao-hui;LUO Zhi-min;ZHAO Bing
Isolated microspore culture was carried out by ten varieties（strains）of kohlrabi（
Brassica oleracea
L. var.
caulorapa
DC.）. It was acknowledged that the donor plant genotype is the key factor affecting microspore culture in kohlrabi. There were significant differences existing in embryoid inductions with different genotypes. Initial flowing stage was the optimum drawing time. Adding suitable quantity of hormone could raise embryoid induction rate. The suitable concentration of 6-BA was 0.2 mg·L
-1
. The effects of NAA were different according to different genotypes. Pretreatment of 4 ℃ low-temperature for 24 h could increase the frequency of embryoid induction and adding 0.25 mg·mL
-1
active carbon could significantly promote embryoid induction and improve embryoid development.
2010 Vol. 1 (10): 35-39 [
Abstract
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1139
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349KB] (
596
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40
Studies on Dynamic Changes of Endogenous Hormone in Sprawl Cucumber and Dwarf Cucumber
HU Bao-zhong;MENG Jing;LI Feng-lan;HU Xiao-mei;YUAN Qiang
In this study, sprawl cucumber（
Cucumis sativus
L.）129 and dwarf cucumber D0462 were taken as experimental materials. The contents of GA, IAA, ZR and ABA of apical meristems, leaves and roots were investigated, respectively by enzyme-linked immunosorbent assay（ELISA）. The results showed that the contents of GA in D0462 were very significantly lower than that of 129. However, the contents of IAA in D0462 were higher than that of 129 in early growing stages, but significantly lower in mid and late growing stages. The contents of ZR and ABA were very significantly higher than that of 129 in different growing stages. In addition, the ratio of GA/ABA，IAA/ABA and（IAA+GA+ZR）/ABA in D0462 were very significantly lower, but the ratio of ZR/GA were very significantly higher than that of 129. These results indicated that the defect of GA might lead to the dwarf of D0462, and the dwarf was also regulated by multifarious hormones.
2010 Vol. 1 (10): 40-45 [
Abstract
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1152
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1KB] [
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333KB] (
853
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46
Effects of Sulfur on Physiological Characteristics and Main Mineral Elements Absorption in Garlic
LIU Zhong-liang;LIU Shi-qi*;ZHANG Zi-kun
The effects of different sulfur contents on physiological characteristics and absorption of nitrogen, phosphorus and potassium were studied by nutrient solution. The results showed that the plant height, leaf length, leaf area and stem diameter of garlic（
Allium sativum
L.）treated by sulfur were higher than those without sulfur treatment. When sulfur content was 2.25 mmol·L
-1
, the photosynthetic parameter（Pn）and SOD activity in garlic leaf were higher. The POD, CAT and NR activity reached maximum when sulfur content was 1.50 mmol·L
-1
. The analysis showed that the total nitrogen and total sulfur contents in bulb and in bolt positively correlated very significantly with sulfur content in nutrient solution. When sulfur content was 2.25 mmol·L
-1
, the total phosphorus and total potassium contents in bulb and in bolt were higher.
2010 Vol. 1 (10): 46-50 [
Abstract
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1363
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1KB] [
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475KB] (
649
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51
Effects of Different Rootstocks on Fruit Yield , Quality and Resistance to Fusarium wilt of Cucumber
CHEN Zhen-de;WANG Pei-sheng;ZHOU Ying
Taking different cucumber（
Cucumis sativus
L.）rootstock as test material, we studied the effect of rootstock grafting on cucumber yield, quality and resistance to Fusarium wilt. The results showed that the fruit yield of‘Luhuanggua No.3’grafted by 10 different rootstocks could be increased by 40.3 %-68.3 %, among which the yield increasing effect of cucumber grafted by‘Shenli No.1’was the best,‘Huangzhen No.3’,‘Xindongli’ and ‘Anamu’took the next place, with yield increasing 50.5 %- 57.5 %. The quality of cucumber with rootstock grafting had no significant effect on cucumber quality, but the contents of VC in ‘Anamu’ and ‘Huangzhen No.4’ were significantly increased. Differences between the rest rootstock grafts and own-rooted seedlings were not significantly. The contents of soluble sugar in ‘Genglishen’, ‘Wolilong’and ‘Huangzhen No.4’ were significantly decreased. But differences between the rest rootstock grafts and own-rooted seedlings were not significantly. The nitrate contents and soluble protein in rootstock grafts were almost the same as that in own-rooted seedlings. Incidence of Fusarium wilt of the rootstock grafted was 0-8.3 %, which was obviously lower than the own-rooted（29.5 %）. As a result,‘Shenli No.1’and‘Huangzhen No.3’are firstly considered as rootstock varieties, and‘Xindongli’and‘Anamu’are taking the second place.
2010 Vol. 1 (10): 51-54 [
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1179
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256KB] (
857
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55
Effect of Different Cooking Method and Refrigeration Period on Contents of VB
1
，VB
2
，VC in
Allium
Vegetables
LIU Hui;ZHOU Rui-hua;GAO Shang;NING Hong-zhen;TANG Yong-mei;ZHANG Ai-juan*
Four
Allium
vegetables were processed by common cooking methods and refrigeration in different time periods after cooking. VB
1
, VB
2
and VC contents before and after cooking and refrigeration were measured by GB. The results indicated that different cooking methods could bring different effects on contents of 3 kinds of vitamins. Frying caused the smallest loss of vitamins and steaming had the biggest loss. 3 kinds of vitamins had different degrees of loss during refrigeration. The longer the refrigerated period, the bigger loss of vitamin.
2010 Vol. 1 (10): 55-58 [
Abstract
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1394
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1KB] [
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285KB] (
695
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59
Humidity Test of Chinese Cabbage Black Rot Identification and Correlation Analysis of Its Seedling and Adult-plant Resistance
ZHAI Wen-hui;ZHANG Tao-tao;HU Jun;ZHANG Feng-lan;YAN Hong*
Taking Chinese cabbage〔
Brassica campestris
L. ssp.
pekinensis
（Lour）olsson〕‘Beijingxin No.4’, ‘Beijingxin No.1’, ‘Beijingdaniuxin’, ‘Jingcui No.60’, ‘Jingcui No.70’, ‘Jingqiu No.65’as tested materials, humidity test and correlation analysis of Chinese cabbage seedling and adult-plant resistance to black rot were studied. The result indicated that there existed significant differences among different Chinese cabbages for black rot resistance and‘Beijingxin No.1’had the strongest resistance to black rot. Comparing disease indexes under different wet conditions, we found moisture was the key factor affecting black rot occurances. Using DPS software to conduct regression analysis and analyzing the correlation between artificial inoculation disease index of black rot in seedling stage and in adult plant stage, we found the results were that there existed notable linear correlation between artificial inoculation in seedling stage（before inoculation no moisture and after inoculation moisture for 24 h）and the disease index in adult-plant stage, and the method of artificial inoculation in seedling stage to identify resistance to black rot is reliable.
2010 Vol. 1 (10): 59-63 [
Abstract
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1240
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1KB] [
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283KB] (
697
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64
Studies on Infectious Condition and Pathogenicity of Tomato
Helminthosporium carposaprum
Pathogen
KANG Li-gong;QI Feng-kun;XU Xiang-yang;LI Jing-fu*
Studies on infectious condition and pathogenicity of
Helminthosporium carposaprum
have indicated that temperature and moisture keeping period can greatly affect the infection of
Helminthosporium carposaprum
. When the temperature was 10 ℃, the pathogen incubation period was 120 h. When the temperature was 25 - 30 ℃, the incubation period was only 74 h. The longer the moisture keeping period, the shorter the being infectious period. The shortest leaf moisture keeping period was about 6 h. The best combination of temperature and moisture keeping period for infection was 25 - 30 ℃ and 42 – 48 h. The susceptibilities of different tomato varieties to Helminthosporium carposaprum were significantly different. 704, 7929, 7913 were susceptible varieties, 08HN31, 7945, 08HN30 were resistant varieties. There existed differences in pathogenicities of different seedling ages. The pathogenicity of 10 days age was the strongest, while that of 5 days or 20 days ages were the weakest.
2010 Vol. 1 (10): 64-67 [
Abstract
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1278
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1KB] [
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299KB] (
643
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68
Co-toxicity of Tea Saponin and Mancozeb against
Colletotrichum capsici
HAO Wei-ning;CHEN Wen-tuan;HU Mei-ying*;LI Hui;YANG Liu;GENG Peng
The co-toxicity and its synergistic ratios（SR）of tea saponin and mancozeb to
Colletotrichum capsici
was determined by using mycelium growth rate method, spore germination method and co-toxicity coefficient method. Meanwhile, the controlling effect in indoor pot experiment on colletotrichum capsici and its mixture was also tested. The results showed that the best mixed ratio of tea saponin and mancozeb to inhibit mycelium growth and spore germination of Colletotrichum capsici was 3∶7, and its SR were 2.45 and 2.48, respectively. The pot test results indicated that the controlling effect of the mixture at 3∶7 ratio was obviously superior than that of the individual treatment of the 2 fungicides.
2010 Vol. 1 (10): 68-71 [
Abstract
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1572
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1KB] [
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299KB] (
988
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72
Effect of Appling
Trichoderma aureoviride
and Muti-microorganisim on Soil Fertility and Soil Enzyme Activity
SUN Dong-mei;LIN Zhi-wei;DUAN Dong-ping;CHI Li;ZHANG Shu-quan;YU Yun-kai;WANG Zhuo
Taking antagonism bacteria, phosphate dissolving bacteria, silicate dissolving bacteria and
Trichoderma
sp. as material，through pot experiment, we compared the results and studies the effects of different bio-fertilizer on soil nutrients and soil enzymes activities. The results showed that the concentration of soil nutrients in the treatment of bio-fertilizer were higher than that in the treatments of pure water and chemical fertilizer. The soil urase and phosphatase activity were detected at different tomato development stages. The result showed that the change tendency of soil urase activity and phosphatase activity were the same along different tomato growing stages, the enzyme activity was increasing from germination stage to flowering stage, but decreasing after flowering stage. Contrast to other treatments, the enzyme activity of bio-fertilizers mixed treatment（phosphate dissolving bacteria, silicate dissolving bacteria, antagonism bacteria and
Trichoderma
sp.）was higher. The urase activity and phosphatase activity by different bio-fertilizer treatments were higher than the treatment of pure water and chemical fertilizer. The urase activity and phosphatase activity of treatment E were 30 % and 27 % higher than that of the treatment of chemical fertilizer, respectively. However, the differences in catalase activity and cellulase activity among different treatments were not significant after harvest.
2010 Vol. 1 (10): 72-76 [
Abstract
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1300
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1KB] [
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296KB] (
720
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77
Effects of Straw Pot Seedling on Growth and Yield of Watermelon
SUN Yu-dong;HUANG Hong-ying;QIN Jin-hua;LUO De-xu;YANG Hong;ZHAO Jian-feng
Watermelon（
Citrullus lanatus
）seedlings were sowed in pot made from rice straw, and then were transplanted in soil together. The growth indexes of different stages and yield were measured. The results indicated that straw pot can obviously promote the growth of watermelon seedlings. Fresh and dry watermelon weight of roots of the seedlings grew in straw pot were 0.13-0.27 g and 0.02-0.03 g higher than that of the CK, respectively. In terms of the whole growing procedure, watermelon seedlings grew in straw pot gradually showed its advantage. Its yield was 24.7 %-34.6 % higher than that of the CK.
2010 Vol. 1 (10): 77-79 [
Abstract
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1260
) [
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1KB] [
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263KB] (
705
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80
Breeding of Vegetable Soybean Variety —‘Zhexiandou No.4’
ZHU Shen-long;FU Xu-jun;ZHU Dan-hua;LI bai-quan;YUAN Feng-jie
‘Zhexiandou No.4’is a new vegetable soybean developed by Zhejiang Academy of Agricultural Sciences. It is limited setpod, 30-35 cm in plant height. Its node numbers of main stem is 9.7, and the effective branch number is 1.8. Pod number is 30 per plant. The fresh weight of hundred grains is 60 g. The dry weight of hundred grains is 30.1 g. It takes about 81 days from sowing to green pod harvest. The fresh pod yield is about 10.5 t·hm
-2
. The variety is widely adapted to Shanghai, Jiangsu, Anhui, Zhejiang, Jiangxi, Hunan, Hubei and Hainan provinces for spring sowing.
2010 Vol. 1 (10): 80-82 [
Abstract
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1231
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1KB] [
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286KB] (
724
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83
A New Chinese Cabbage F
1
Hybrid —‘Weibai No.7’
HAN Tai-li;YANG Xiao-dong;XU Li-gong;TAN Jin-xia;SONG Yin-hang
A new variety of Chinese cabbage‘Weibai No.7’was bred by crossing two self-incompatible lines BZ-02-17 and BZ-02-10. It can be harvested in 65 days after sowing. The plant is 38 cm in height and 50 cm in width. Its leaf is dark green in color and petiole is white. The head is 32 cm in height and 21 cm in diameter. The average plant weight is 5.7 kg and the net product ratio is 73.6 %. Its net output got to 99.3 t·hm
-2
. It is resistant to TMV and downy mildew. It is fit for planting in early autumn in Shandong, Heilongjiang, Jilin, etc.
2010 Vol. 1 (10): 83-84 [
Abstract
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1075
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213KB] (
607
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85
A New Watermelon F
1
Hybrid —‘Huazhixiu’
SUN Shou-ru;ZHANG Ju-ping;MA Chang-sheng;*;SUN Zhong-wei;ZHU Wei-ling
‘Huazhixiu’is a new watermelon F
1
hybrid with medium maturity. It was developed by crossing M02-10 as female parent and JF-11 as male parent. Its growing period is about 105 days from sowing to harvest. Its fruit matures about 32 days after pollination. The first fruit is set at 8 th-10 th nodes. The fruit is elliptical shaped with dark-green wide stripes on green skin. The sphericity index is 1.38. Its flesh is red, juicy and crispy. It tastes very sweet. The pericarp is hard to crack. It is suitable for storage and transportation. The average fruit weight is about 8.5 kg and the yield is 46.5 t·hm
-2
. This variety has good adaptability and can be grown in main watermelon production areas in China.
2010 Vol. 1 (10): 85-87 [
Abstract
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1134
) [
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1KB] [
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234KB] (
651
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88
A New Muskmelon F
1
Hybrid —‘Hongxing’
PANG Guo-xin;SHEN Ai-min;MA Jian-hui;GUO Zeng-zhi;ZHANG Gong
Hongxing’is a new mid-early muskmelon F
1
hybrid developed by crossing inbred lines 0801（female parent）and Jin20-1（male parent）. It takes 75-90 days after sowing to harvest and 33-35 days after flowering to fruit maturity. It has a middle growth vigor and strong stress resistance. It is highly resistant to downy mildew and moderately resistant to gummy stem blight and resistant to powdery. Its fruits are round, smooth and bright yellow. Its flesh is red, crisp, sweet, good smell and the thickness is 4.0-4.5 cm. The fruits have an excellent quality, good taste and its total soluble solids content is 15.5 %. Its average fruit weight is 2.0 kg and average yield is 60 t·hm
-2
.‘Hongxing’is suitable to be cultivated in protected field in North China. Its planting density is 2 000 plants per 667 m
2
.
2010 Vol. 1 (10): 88-90 [
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1143
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1KB] [
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310KB] (
793
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