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  Current Issue
2011 Vol.1 Issue.4,Published 2011-02-15

1 Research Progress on Physiological Effect of Heavy Metals in Vegetables and Their Accumulation Rule
ZOU Ri;SHEN Di;BAI Xin-fu;LI Xi-xiang
Vegetables could provide essential vitamins and minerals for human being and they were necessary for people’s daily diet. Along with the rapid development of industry and ever-growing globally contamination, vegetable products were subject to hazards of different degree. Heavy metal pollution was one of important factors that affected vegetable growth and human health. Based on previous studies, this paper briefly introduced the major sources of heavy metals from 2 aspects of soil and atmosphere, and analyzed the effect of heavy metals on vegetable plant growth, cell ultra-structure, membrane permeability, enzyme activity, photosynthesis, proline content, and their physiological and biochemistry effects. The paper also expounded the accumulation rule of heavy metals in different vegetable crops, varieties and vegetable organs. It carried out preliminary discussion on their molecular mechanism.
2011 Vol. 1 (4): 1-7 [Abstract] ( 1158 ) [HTML 1KB] [ PDF 287KB] ( 657 )
8 Preliminary Identification of S Haplotypes of Two Self-incompatible Lines in Cabbage(Brassica oleracea var. capitata L.)
TIAN Lei;ZHUANG Mu*;LIU Yu-mei;YANG Li-mei;ZHANG Yang-yong;FANG Zhi-yuan
According to the conserved sequence of S locus kinase(SRK)gene in cabbage(Brassica oleracea var. capitata L.), a pair of SRK-specific primers was designed. The S haplotypes of 2 inbred lines in cabbage were identified by PCR amplification and DNA sequencing, combined with bio-informatics analysis. BLAST results indicated that the self-incompatible line 96-100 showed 91 % similarity in nucleotide sequence with SRK29 gene in cabbage, and 100 % similarity under 84 % query coverage of protein residue with S14 haplotype in cabbage, while the self-incompatible line Eyin165 exhibited 100 % similarity in nucleotide sequence with BoSRK28 gene in cabbage. This indicated that S haplotype of Eyin165 is S28.
2011 Vol. 1 (4): 8-12 [Abstract] ( 1317 ) [HTML 1KB] [ PDF 348KB] ( 546 )
13 SRAP Marker Analysis of Recessive Genic Male Sterile Restoring Gene in Chinese Cabbage
ZHANG Hui;ZHANG Shu-jiang;LI Fei;ZHANG Shi-fan;SUN Ri-fei*
Genic male sterility lines were widely used in F1 seed production in Chinese cabbage(Brassica rapa L. ssp. pekinensis). A recessive genic male sterile line 454AB which contained 320 individuals was constructed for this study. SRAP techniques and bulked segregant analysis(BSA)were used to screen markers linked to the RGMS restoring gene. Among the 1 128 primer combinations, only BMe10SA4 and M52K2 showed polymorphism between bulks of male sterile and fertile. The genic distances between restoring gene with BMe10SA4 and M52K2 were 4.35 cM and 7.74 cM, respectively.
2011 Vol. 1 (4): 13-16 [Abstract] ( 1196 ) [HTML 1KB] [ PDF 534KB] ( 532 )
17 Cloning and Sequence Analysis of LFYZQ from Brassica oleracea L. var. capitata L.
TANG Qing-Lin;WANG Zhi-Min;REN Xue-song;SONG Ming*;WANG Xiao-Jia
DNA and RNA were extracted from bolting stem apex of cabbage(Brassica oleracea L. var. capitata L.)cultivar ZQ and LFYZQ gene fragments were cloned by RT-PCR respectively with 2 pairs of primers. LFYZQ of DNA and cDNA sequence fragments assembly were respectively 2 560 bp and 1 239 bp. Analysis of the nucleotide sequence by BLAST on line indicated that homology of LFYZQ was up to 91 % between Brassica oleracea L. var. capitata L. and Brassica oleracea L. var. botrytis L.,up to 87 % between it and Arabidopsis thaliana, up to 86 % between it and Brassica juncea Coss., and up to 87 % between it and Raphanus sativus L. Analysis of gene structure by DNAstar indicated that LFYZQ including 3 extrons(452, 394, 393 bp)which totally coded 412 amino acids and 2 introns(514, 807 bp)which were unanimous with nucleotide splicing rules of GT-AG. Analysis of amino acids among LFYZQ and other 12 kinds of Brassicaceae LFY on line were divided into 2 classes: class Ⅰ with 3 plants(Brassica oleracea var. botrytis L., Brassica oleracea var. capitata L., Jonopsidium acaule)and class Ⅱ with other 10 plants. Molecular relativity quality of LFYZQ was 46 kD. LFYZQ was an unstable and hydrophobic protein with 4 kinds of active sites: N-myristoylation site, protein kinase C phosphorylation site, Casein kinase Ⅱ phosphorylation site and Amidation site.
2011 Vol. 1 (4): 17-22 [Abstract] ( 1157 ) [HTML 1KB] [ PDF 590KB] ( 666 )
23 Preliminary Analysis of Cucumber Submergence Tolerance at Seedling Stage
QI Xiao-hua;CHEN Rong-feng;XU Qiang;CHEN Xue-hao
To analysis submergence tolerance of 40 cucumber(Cucumis sativus L.)cultivars, the seedlings of each line at five-leaf stage were treated with flooding water. The plant death rate, chlorophyll loss ratio, SOD, POD and CAT values were checked at the 0 and 6 days after treatment. Based on the average of subordinate function(SF)analysis, North China cucumber showed high tolerance to submergence treatment and South China cucumber was middle tolerant to submergence, but zaoer N line has high tolerance to submergence. American field cucumber and European greenhouse cucumber showed low tolerance or susceptible to submergence. Two Japanese cucumber lines and one West Asia cucumber line were used in present evaluation. They showed middle tolerance and susceptible to submergence treatment.
2011 Vol. 1 (4): 23-28 [Abstract] ( 1300 ) [HTML 1KB] [ PDF 213KB] ( 472 )
29 Effects of Different Leggy Extent Seedlings on Cucumber Growth,Yield and Quality
MING Cun-hao;JIANG Fang-ling;HU Hong-min;ZHOU Xue-chao;ZHAN Feng-hua;WU Zhen*
Using 2 different ecotypes of cucumber(Cucumis sativus L.)variety‘Jinchun No.4’and ‘Daiduoxing’as materials to obtain a couple of cucumber seedlings with different leggy extent by shading, the effects of seedlings leggy extent on cucumber plant growth, fruit yield and quality were studied. The results showed that different degrees of cucumber seedlings leggy growth occurred under shading conditions, and planting had significant impact on plant growth, fruit yield and quality. The plant height and hypocotyl length of leggy seedlings were significantly higher than that of the control, but the stem diameter, leaf area, dry weight, dry matter content, root/shoot ratio and seedling index all displayed opposite changing trend. After planting, the plant height,stem diameter, leaf number and leaf area of leggy seedlings were significantly differed from those of the control seedlings. Compared with the control, the leggy seedlings delayed its early blooming period; the node of its first female flower was higher; while the average pod number per plant, fruit weight, and early yield per plant and per unit area were all significantly decreased; its fruit soluble solids, soluble sugar, soluble protein and VC contents were also significantly reduced. This suggested that after planting cucumber leggy seedlings had significant negative effects on plant growth, fruit yield and quality.
2011 Vol. 1 (4): 29-34 [Abstract] ( 1351 ) [HTML 1KB] [ PDF 233KB] ( 460 )
35 Correlation and Clustering Analysis of Leaf Color Parameters in Brassica campestris L. ssp. chinensis(L.)Makino
LIU Wei-xin;LOU Yan
In this study, leaf color parameters of 19 cultivars of Brassica campestris L.ssp.chinensis(L.)Makino(including 3 variants e. g. var. communis Tsen et Lee., var. rosularis Tsen et Lee., and var. taitsai Hort)were determined using a colorimeter and a chlorometer. Correlation and clustering analysis were carried out based on these data. The results showed that significant differences in chlorophyll content and leaf color parameters were found for the tested cultivars. Color parameters including h and C were correlated with content of chlorophyll. Based on the results of clustering analysis, the 19 Brassica campestris L. ssp. chinensis(L.)Makino cultivars were divided into 2 groups with one had light leaf colors, and the other had dark leaf colors. Cvs.‘Lvxiu’,‘PAK’,‘Aijiaohuang’,‘Tadiwu No.3’and‘Jingpintadiwu’were very similar to Japanese cvs.‘Xiadi’,‘Huaguan’, and‘Dongshangwei. The advantages of this method were easy operating, cost and time-saving, and harmless to leaves.
2011 Vol. 1 (4): 35-38 [Abstract] ( 1308 ) [HTML 1KB] [ PDF 198KB] ( 496 )
39 Effects of Fertilizer and Sulfate Treatment on Phytochelatins Content in Zizania latifolia under Cadmium Stress
HUANG Kai-feng;;JIANG Jie-zeng*
Content of non-protein-thiols(NPT), glutathione(GSH)and phytochelatins(PCs)in leaves and roots of Zizania latifolia were studied on single-harvested cultivar‘Jiangshujiao’and double-harvested cultivar‘Fenghongzao’. These 2 cultivars were cultured in soil with organic fertilizer(OF)and inorganic fertilizer(IF)at different concentrations of sulfate treatment under 100 mg?L-1 Cd2+ stree. The results showed that content of NPT, GSH and PCs in Zizania latifolia leaves and roots were increased when treated with sulfate under cadmium stree. Content of GSH and PCs were higher when treated with IF than OF. These contents in Zizania latifolia roots were remarkably higher than in leaves. Contents of NPT, GSH and PCs of‘Jiangshujiao’were higher than that of‘Fenghongzao’in leaves and roots at the same treatment.
2011 Vol. 1 (4): 39-43 [Abstract] ( 1160 ) [HTML 1KB] [ PDF 218KB] ( 456 )
44 The Effects of Adding secondary metabolites of Aspergillus niger on Desease Resistance to Root-knot Nematode of Tomato
LI Song;DUAN Yu-xi;ZHU Xiao-feng;CHEN Li-jie;WANG Yuan-yuan
In order to define the secondary metabolites of Aspergillus niger. to tomatoes root-knot nematode (Meloidogyne incognita.) disease prevention effect and the change of defense enzyme activity ,application of various concentrations of secondary metabolites of A niger. to root-knot nematode disease in tomato were studied in greenhouse. And the research on effects of secondary metabolites on several defense enzymes in tomato was investigated. The results showed that the secondary metabolites of A. niger. reduced the root-knot index and nematode populations,meanwhile promoted tomato plant growth compared with untreated control. The activities of defense enzymes enhanced significantly, such as phenylalanine ammonia (PAL), polyphenol oxidas (PPO), peroxidase (POD), superoxide (SOD) and catalase (CAT). It indicated that the secondary metabolites of A. niger. could markedly induce the resistance of tomato to root-knot nematode and have great potential in biocontrol.
2011 Vol. 1 (4): 44-49 [Abstract] ( 1545 ) [HTML 1KB] [ PDF 293KB] ( 560 )
50 Effects of Microorganism Agent on Growth and Nutritional Quality of Brassica campestris L. ssp. chinensis(L.)Makino var. communis Tsen et Lee and Soil Condition
LI Zhen-zhen;ZHOU Xiao-guang;ZHU Chun-yan;NIU Qing-liang;HUANG Dan-feng*
This research focuses on the effects of different levels of microorganism agent-Zhibaokang on the growth, nitrate content, nutritional quality of Brassica campestris L. ssp. chinensis(L.)Makino var. communis Tsen et Lee and soil condition. Results showed that microorganism agent had significant effect on the leaves number, maximum leaves area and yield of Brassica campestris L. ssp. chinensis(L.)Makino var. communis Tsen et Lee,especially at a concentration of 22.5 kg?hm-2 level, and the plant had the lowest nitrate content at 22.5 kg?hm-2 level. But for the content of VC, 15.0 kg?hm-2 level was the highest. Furthermore, the soil salt content was reduced and the microbial number increased significantly with the addition of microorganism agent-Zhibaokang. In conclusion, microbial agents at concentration of 22.5 kg?hm-2 level has the optimal economic effect.

2011 Vol. 1 (4): 50-55 [Abstract] ( 1233 ) [HTML 1KB] [ PDF 356KB] ( 516 )
56 Determination of 21 Bactericides Toxicities on Xanthomonas campestris pv. vesicatoria
REN Jian-guo;;WANG Jun-li;;YUE Mei-yun
Twenty-one bactericides were used to assay the toxicity to Xanthomonas campestris pv. vesicatoria, which could provide references for the selection of bactericides in felids. The results showed that streptomycin, dichloroisocyanuric acid sodium and streptomycin+oxyteracykine had obvious toxicity to Xanthomonas campestris pv. vesicatoria, and their EC50 value were 35 582, 925 186 and 1 948 mg?L-1, respectively. Certain concentration proportional streptomycin and streptomycin + oxyteracykine, dichloroisocyanuric acid sodium and streptomycin + oxyteracykine had the synergic effects on the toxicity to Xanthomonas campestris pv. vesicatoria, while dichloroisocyanuric acid sodium and streptomycin showed independent effects on the toxicity to Xanthomonas campestris pv. vesicatoria.
2011 Vol. 1 (4): 56-60 [Abstract] ( 2299 ) [HTML 1KB] [ PDF 776KB] ( 714 )
61 Diagnosis and Control of Bean Sprouts Rhizoctonia solani
ZHANG Li;ZHANG Li-qun;DUAN Hui-mei;KANG Yu-fan*;LV Yu-lan
Simulating factory production process of sproutsing to cultivate bean sprouts, taking spoilage sprouts samples during the process of cultivating, then separating, purifying and identification were carried out to determine the pathogen. Finalized for the pathogen Rhizoctonia solani was finalized. Observing the pathogenicity of inoculation volume of different Rhizoctonia mycelial suspensions on bean sprouts was tested. The results showed that different inoculums leaded to the differencest of disease occurring the time of incidence, the incidence rate and bean sprouts biological production. Using inoculums at 2 pieces bacteria per 5 milliliter and 2 pieces bacteria per 10 milliliter of mycelial suspensions to treat bean sprouts, the spoilage of bean sprouts was serious. The incidence rate at the 5th day were 46 %, 25 %, respectively. The results of using different treatments to protect Rhizoctonia solani showed that 40 ℃ hot-water disinfection, 1 % lime water, 1/5 000 potassium permanganate might control the occurrence of Rhizoctonia to some extent, the relative control efficiencies were 91.30 %, 54.35 %, 50.00 %, respectively.
2011 Vol. 1 (4): 61-65 [Abstract] ( 2385 ) [HTML 1KB] [ PDF 862KB] ( 642 )
66 Effects of Grafting on Bitter Gourd Resistance to Phytophthora Blight, Yield and Quality
XIAO Chang-hua;KUANG Bi-feng;YU Xi-mao;TAN Mei-li;DENG Xian-chao;TANG E;OUYANG Feng
We studied the survival rate of grafted bitter gourd(Momordica charantia L.), and effects of grafting on plant growth and development, phytophthora blight controll, its yield and product quality by 4 rootstocks. The results showed that these 4 rootstocks grafted with‘Xiangzaoyou No.1’had no effect on the plant chlorophyll content, fruit yield and quality, but had good controlling effect on phytophthora blight. Among the 4 rootstocks,‘Weilong No.1’had the best grafting effect. Its survival ratio reached 98 % and blight morbidity was only 1.7 %, disease index was 3.4. Its yield increased 63.2 % than the self-rooted plant.
2011 Vol. 1 (4): 66-69 [Abstract] ( 1665 ) [HTML 1KB] [ PDF 201KB] ( 462 )
70 Effect of Different Concentrations of Garland Chrysanthemum Organ Water Extract on Seed Germination Characteristics of Watermelon
FAN Shu-ying;XIAO Xu-feng;XIONG Chun-hui;NI Xiao-chun;WU Cai-jun*
In order to study the effects of different concentrations of garland chrysanthemum organ water extract on seed germination characteristics of watermelon(Citrullus lanatus), 0.001, 0.01 and 0.1 g?mL-1 organ water extracts from garland chrysanthemum root, stem and leaf, flower were used to treat watermelon seeds and to test seed germination characteristics. The results showed that different concentrations of garland chrysanthemum root, stem and leaf, flower water extracts could inhibit seed germination of watermelon. The inhibitory effect increased along with the concentration rise. The germinability, germination percentage, radicle quality were all lower than the control. Therefore, 0.001-0.1 g?mL-1 garland chrysanthemum organ water extracts has distinct allelopathy effect on watermelon germination.
2011 Vol. 1 (4): 70-72 [Abstract] ( 1554 ) [HTML 1KB] [ PDF 174KB] ( 526 )
73 A New Cucumber Rootstock F1 Hybrid —‘Jingxinzhen No.5’
ZHANG Fan;JIA Chang-cai;JIANG Li-gang;ZHANG Guo-yu;LI Hai-zhen*
‘Jingxinzhen No.5’is a hybrid cucumber rootstock bred by taking N15 as female parent and N11 as male parent. It is of high and uniform germinate rate with strong seedlings and well developed root system. It has good fertilizer absorbing ability and high grafting affinity. Its survival ratio is high. It is tolerant to low temperature and weak light, and resistant to Fusarium wilt. It can yield 5.5 % higher than‘Lingxiu’. After grafting with cucumber, its fruit is with shiny dark green skin and good quality. It is adapted to protected cultivation in early spring and for over-winter.
2011 Vol. 1 (4): 73-75 [Abstract] ( 1295 ) [HTML 1KB] [ PDF 171KB] ( 527 )
76 A New Muskmelon F1 Hybrid —‘Gantianyulu’
LIU Dong-shun;SU Yong-quan;LIU Sheng-feng;CHENG Hong;YANG Wan-bang;KONG Wei-ping
‘Gantianyulu’is a mid-early maturity F1 hybrid of muskmelon developed by crossing 03W05 as female parent and 03W01 as male parent. The fruit matures about 96 days from sowing and 40 days from pollination. The average fruit weight is 2.0 kg. Its fruit is of oval shape with white shin. The sugar content is about 16 %. Its yield is 48 t?hm-2. It is suitable for cultivation in open field in Northwest areas and for protected cultivation in other regions of China.
2011 Vol. 1 (4): 76-77 [Abstract] ( 1927 ) [HTML 1KB] [ PDF 135KB] ( 465 )
78 A New Early Maturing Tomato F1 Hybrid —‘Zhengfan 06-10’
YING Fang-qing;HUANG Wen;HUANG Xiao-yan;LI Wu-gao
‘Zhengfan 06-10’is a new tomato F1 hybrid developed by crossing 2 inbred lines 04 shangyinchanggui-1-hun-hun as female parent and 05 zaofenfuben-hun as male parent. Its young fruit is with slightly green shoulder and the ripened fruit is of blight pink color. The fruit is of round shape. The average single fruit weight is 153 g. The field investigation indicates that it is resistant to leaf mold and virus disease similarly to‘Zhongzha No.9’. The malformation and cracking rate is 2.22 %. Its fruit is hard with uniformity. They are durable for storage and transportation. The soluble solids content is 4.8 %. VC content of fruit flesh is 294 mg?kg-1. The total acid content is 0.42 % and the soluble total sugar content is 2.58 %. It has superior quality and comprehensive characteristics. The yield is 96 t?hm-2.
2011 Vol. 1 (4): 78-80 [Abstract] ( 1243 ) [HTML 1KB] [ PDF 205KB] ( 616 )
81 A New Tomato F1 Hybrid —‘Gangyu No.1’
HE Guo-chang;LIANG Jin;LIU Yu;HUANG Ying-lan;HUANG Zhang-hua;LIU Ce;YANG Sheng-gan;CHEN Guo-dong
‘Gangyu No.1’is a new tomato F1 hybrid developed by crossing GS619-54 as female parent and ZX1-53 as male parent. It is of unlimited growth type with strong growth vigor. The first inflorescence node sets on section 6-8. Its floral interval is 2-3 nodes. Its fruit is of flat round shape. The ripe fruits are of bright red color. The average fruit weight is 160 g. The soluble solids content is 5 %, VC content is 220 mg?kg-1. The total sugar is 4.0 % and the sugar acid ratio is 5.1. The malformation and cracking rate is 9.7 %. It has high commodity rate. Its fruit is hard and durable for storage and transportation. It is resistant to bacterial wilt and mid-resistant to virus disease. The average yield is about 112.5 t?hm-2.
2011 Vol. 1 (4): 81-82 [Abstract] ( 1141 ) [HTML 1KB] [ PDF 126KB] ( 467 )
83 A New Hot Pepper F1 Hybrid Specially for Drying Use —‘Hangjiao No.10’
HUO Jian-tai;LUO Ai-yu*;GAO Yan-hui;WEI Jin-bin;LI Xiang-rong;HU Xiao-ming;ZHANG Jian-dong;PU Yong-hong
‘Hangjiao No.10’is a new hot pepper F1 hybrid specially for dry use. It is developed by crossing inbred lines 022-5-1 and 023-3-2. It has vigorous growth and middle maturity. It has about 34 fruits per plant. The fruit is of long tapered shape. The dried peper is purplish red in color with strong pungent flavor. It is 13.6 cm in length, 1.6 cm in diameter, 2.6 g in weight. This cultivar is resistant to Phytophthora capsici Leonian and virus disease. The average dried-pepper yield is 5.25 t?hm-2. It adapts to open field cultivation in northwest and North China planting.
2011 Vol. 1 (4): 83-85 [Abstract] ( 1505 ) [HTML 1KB] [ PDF 207KB] ( 531 )
86 A New Hot Pepper F1 Hybrid —‘Yangjiao No.1’
ZHOU Ru-mei;QI Jian-bo;PENG Han-gen;ZHANG Yong-tai;LI Ai-min;ZHANG Ying
‘Yangjiao No.1’is a new hot pepper F1 hybrid developed by crossing female parent 95079-3-2 with male parent 94041-3-55. It is of early maturity. The fruit is long lantern-shaped with length of 14.0-17.5 cm. Its should diameter is 4.6 cm, flesh thickness is 0.31 cm. The average fruit weight is 60.8 g. It is slightly hot and has high marketing quality. It has resistance to virus. Its yield is 45.0-52.5 t?hm-2. It is suitable to be cultivated both in early spring protected and spring and autumn open fields in Jiangsu Province.
2011 Vol. 1 (4): 86-87 [Abstract] ( 1051 ) [HTML 1KB] [ PDF 129KB] ( 469 )
88 A New Fl Hybrid Flowering Chinese Cabbage —‘Chaoza No.13’
GAO Can-hong;ZHOU Kai;XU Fu-le;SHEN Ji-chang;ZHENG Ting-ping
‘Chaoza No.13’is a new mid-maturity F1 hybrid variety of flowering Chinese cabbage developed by crossing GMS0807 as female parent and 9099 as male parent. It has strong growing vigor. The plant is about 30.3 cm in height. Its flower stalk is 20-26 cm in height, 1.2-1.8 cm in diameter and 40-60 g in average weight. It has uniform and compact corm with dark green and shining color. It can be harvested 35-38 days after sowing and harvest can last for 5-7 days. Its yield is generally about 12 t?hm-2. It has sweet taste, less fiber, high quality and strong resistance to stress. It is suitable for cultivation in South China.
2011 Vol. 1 (4): 88-90 [Abstract] ( 1230 ) [HTML 1KB] [ PDF 178KB] ( 498 )
91 A New Winged Bean Variety —‘Xianglingdou No.2’
JIANG Xiang-hui;CHEN Dong-ming;QUAN Miao-hua
‘Xianglingdou No.2’is a new winged bean variety bred through systematic selection for 6 successive generations by crossing‘Zhongyi No.1’as female parent with K0000026 as male parent. It was suitable to plant at late spring and early summer. This variety has many lateral branches. It has small leaves, uniform pods, good and successive fruit bearing ability. It is tolerant to heat. Its pod color is dark green with shine. The pod is about 20 cm in length, 3 cm in width. The single pod weights 35-45 g. Its pod is of good quality. Its fresh pod yield is 8.212 5 t?hm-2.
2011 Vol. 1 (4): 91-93 [Abstract] ( 1194 ) [HTML 1KB] [ PDF 175KB] ( 528 )
2011 Vol. 1 (4): 94-94 [Abstract] ( 882 ) [HTML 0KB] [ PDF 112KB] ( 572 )
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