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Flow Cytometric Analysis of Ploidy Level of Regenerated Plants Obtained by Anther Culture in Radish(Raphanus sativus L.)and Field Obtained |
Xiong Qiufang,Zhang Xueqing,Rong Kaifeng |
Wuhan Vegetable Institute of Science; Hubei 430065; College of Biotechnology; Wuhan Engineering University |
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Abstract Flow cytometic(FCM)analysis of ploidy level was made with twenty radish regenerated plants obtained by anther culture.Numbers of haploid,diploid,tetra-ploid and chimera were learned from the DNA content of the regenerated plants tested.In the analysis,haploids,diploids,tetra-ploids and chimeras covered 20 %,30 %,25 % and 25 % respectively.The ploidy levels of regenerated plants were identical to that of the plants grown in the field.
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Received: 19 May 2007
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