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| Several Factors Affecting Embryoid Induction from Isolated Microspore in Kohlrabi（Brassica oleracea L. var. caulorapa DC.） |
| WANG Chao-nan, WEN Feng-ying, LIU Xiao-hui, LUO Zhi-min, ZHAO Bing |
| Tianjin Kernel Vegetable Research Institution, Tianjin 300384, China |
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Abstract Isolated microspore culture was carried out by ten varieties（strains）of kohlrabi（Brassica oleracea L. var. caulorapa DC.）. It was acknowledged that the donor plant genotype is the key factor affecting microspore culture in kohlrabi. There were significant differences existing in embryoid inductions with different genotypes. Initial flowing stage was the optimum drawing time. Adding suitable quantity of hormone could raise embryoid induction rate. The suitable concentration of 6-BA was 0.2 mg·L-1. The effects of NAA were different according to different genotypes. Pretreatment of 4 ℃ low-temperature for 24 h could increase the frequency of embryoid induction and adding 0.25 mg·mL-1 active carbon could significantly promote embryoid induction and improve embryoid development.
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Received: 04 January 2010
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Corresponding Authors:
WANG Chao-nan
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