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| Studies on Isolation of Resistance Related-genes to Black Rot by Suppression Subtractive Hybridization in Brassica oleracea |
| ZHU Yan, WANG Chao* |
| College of Horticulture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China |
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Abstract In this research, suppression subtractive hybridization（SSH）was adopted to
construct a mixed SSH library by inducing black rot to disease resistant cabbage（Brassica oleracea L.）variety A21 for 6h, 8h, 12h and 24h. Through reverse northern screening, 87 ESTs with high quality were obtained. After conducting comparative analysis with BLAST data bank in NCBI, 83 of the ESTs were found to have homology by different degrees with known protein or genes.
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Received: 04 January 2010
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Corresponding Authors:
WANG Chao
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