|
|
|
| Biological Identification of Low Temperature Tolerance Gene Transform of Cucumber and Its Progeny |
| LU Shu-wen1, LIU Wen-ping1, LI Zhu-gang1, NAN Xiang-ri1, LIU Jian-xin1, QIN Zhi-wei2 |
| 1Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China; 2Northeast Agricultural University, Harbin 150030, Heilongjiang, China |
|
|
|
|
Abstract Low temperature tolerance gene BnCS was transformed into cucumber（Cucumis sativus L.）plants. Biological characteristics of transgenic plant（T0）and its progeny（T1）were biologically identified. Under low temperature stress at 13 ℃, the germination potential, germination percentage and radicle length of transgenic T0 seed were significantly higher than the CK（cultivar yd-36）. The percentage of electrolyte leakage and MDA content（T1）were significantly lower than the CK. While the increment growth of plant height, stem width and leaf area were higher than the CK. This indicated that low temperature tolerance of transgenic plants was obviously higher than that of the non-transgenic plants.
|
|
Received: 21 December 2009
|
|
|
|
Corresponding Authors:
LU Shu-wen
|
|
|
|
|
|
|
