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| Molecular Identification of Co-infection of Tomato Spotted Wilt Virus and Southern Tomato Virus in Yinchuan Region of Ningxia |
| KANG Huajun1，3，LI Jianshe2，GAO Yanming2，SHI Juan2，SHI Yanxia1，XIE Xuewen1，LI Lei1，FAN Tengfei1，LI Baoju1*，CHAI Ali1* |
| 1Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；2Ningxia University，Yinchuan 750021，Ningxia，China；3Gansu Agricultural University，Lanzhou 730070，Gansu，China |
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Abstract During disease investigation in Yinchuan region of Ningxia in 2021，we found suspected virus disease symptoms of brown and irregular spot on tomato plant stems and unmatured fruits．By Trizol method，we extracted the total RNA of infected tomato plant samples，and conducted RT-PCR detection using specific primers of common virus frequently infecting tomato．The results showed that the specific primers of tomato spotted wilt virus（TSWV）and southern tomato virus（STV）had amplified 777 bp and 681 bp target outcomes，respectively．Outcome sequencing，BLAST comparison conducting and Phylogenetic analysis showed that TSWV isolate from Yinchuan Region of Ningxia was on the same branch with TSWV isolate from Spanish pepper．Two of them had the closest relation．STV isolate had the closest evolutionary relation with STV isolates on tomatoes from France，Pakistan and Colombia，but had farther relation with STV isolates from the other regions．These results indicated that tomatoes in Yinchuan Region of Ningxia were co-infected by TSWV and STV．
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Received: 08 October 2021
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