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| Effects of Vegetable Planting Years on Abundance and Community Structure of Soil Ammonia-oxidizing Microorganism |
| ZHAO Hui，WANG Xiying ，LU Zhihong，TAN Zhiyong |
| College of Economics and Management，Tongren University，Tongren 554300，Guizhou，China |
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Abstract Taking the soil of protected and open vegetable fields planted for 3，5，7 years as research object，this paper studied the effect of planting years of protected vegetable fields on ammonia-oxidizing microorganism abundance and community structure by using real-time PCR and Illumina Miseq high-throughput sequencing technology．The results indicated that the abundance of AOA in different treatments was all higher than that of AOB．The value of AOA/AOB ranged from 17.33-39.16．Along with the prolonging of planting years，the quantity of AOA and AOB decreased gradually．The planting years had significant impacts on the community structure and diversity of AOA and AOB．The both diversity indexes including Chao1 index，ACE index and Shannon index were the maximum，when planting years reached 3．Then，these indexes gradually decreased along with the prolonging of planting years．At the phylum level，the dominant group of AOA communities was Thaumarchaeota．The dominant group of AOB communities was Proteobacteria．Soil pH，ammonium nitrogen，and nitrate nitrogen were the major factors affecting the changes of AOA community structure．In conclusion，during the process of protected vegetable planting，AOA took hold on quantity and community diversity．It was more sensitive to environmental changes，and might be the main driver of nitrification．
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Received: 24 December 2020
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