Taking seed-used pumpkin inbred line ‘98-2’ and meat-used pumpkin inbred line ‘312-1’as test materials，this paper studied on the starch content of pumpkin fruit and expression pattern of starch metabolism related genes．The results indicated that there existed significant differences in accumulation patterns of total starch and amylopectin of‘ 312-1’ and‘ 98-2’ fruits．The accumulation patterns of total starch and amylopectin in ‘312-1’ were similar．They were all accumulated gradually with fruit development，and the peak value appeared in 40 days after pollination，then decreased slightly．While，the contents of total starch and amylopectin in‘ 98-2’ maintained the same level at the early stage of fruit development（5 days after pollination）without significant accumulation process．The contents of total starch and amylopectin in‘ 312-1’were significantly higher than that in‘ 98-2’ during each fruit development stage．The amylose accumulation pattern in the 2 materials was similar，all maintaining at accumulated level at the early fruit development stage．However，the amylose contents in ‘312-1’ at different fruit development stages were significantly or extremely significantly higher than that of‘ 98-2’．In starch composition，amylopectin was dominant in the 2 materials．In addition to ISA Ⅲ，SSSⅡ and SBEⅡ genes，the GPT，AATP，PGM，AGPaseL，GBSSⅠ，SSS Ⅲ and SBEⅠ genes had similar expression trends during fruit development process of these 2 materials，and their expression quantities had large differences．Combining the amylopectin and amylopectin contents in fruits of these 2 materials，the paper speculated that GPT，AATP，AGPaseL，GBSSⅠ，SSSⅡ，SSS Ⅲ，SBEⅠ and SBEⅡ genes were key genes regulating starch accumulation in pumpkin fruit．