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| Establishment of Highly Efficient Regeneration System of Lanzhou Lily |
| CUI Xing-lin1，QIN Xin-hui1*,LIU Fen2，YI Wan-wei1 |
| （1Biological Engineering Department，Jiuquan Vocational and Technical College，Jiuquan 735000，Gansu，China；2Institute of Woods and Flowers，Gansu Province Academy of Agricultural Sciences，Lanzhou 733000，Gansu，China） |
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Abstract Taking fresh Lanzhou lily〔Lilium. davidii Duch. var. unicolor（Hoog）Cotton〕scale as explant，this paper explored the establishment of highly efficient regeneration system．The results showed that the middle and inner layer of the scales were superior than the outer ones，and more suitable to be explant．The scales regeneration frequency reached the highest in medium of MS+0.5 mg·L-1 6-BA+0.5 mg·L-1 NAA，up to 90.0%．A small bud had the highest proliferation rate in the medium of MS+0.8 mg·L-1 6-BA+0.05 mg·L-1 NAA，with an average number of 4.5 per bud new shoots．The medium of MS+0.8 mg·L-1 6-BA+0.05 mg·L-1 NAA+0.5 mg·L-1 AC facilitated the enlargement growth of test-tube plantlets，and their survival rate was over 98% after
transplanting．
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Received: 12 December 2013
Published: 07 January 2014
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