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| Transformation of Fertile Male Sterile AB Lines Type 1 Greens Komatsuna with Purple Petiole and Genetic Characteristics of Petioles’purple Color |
| CHEN Yong-hao，SHEN Xiang-qun*，HONG Ya-ting，MA Shi-fang，LI Lin，GUO Yan-feng |
| College of Horticulture， Shenyang Agricultural University， Shenyang 110866， Liaoning， China |
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Abstract Taking greens komatsuna〔Brassica campestris ssp. Chinensis（L.）Makino〕 with purple petiole and greens komatsuna with green petiole as test materials，we compounded F1、F′1、F2 and BC1F1，and investigated the genetic characteristics of purple petioles．The results show that both petioles of F1、F′1 are purple，but the degree of purple is different，and the separation ratios of petioles color in F2 and BC1F1 are close to 3 purple∶1 green for F2 progenies，1 purple∶1 green for BC1F1 progenies．The result of χ2 test further proved that heredity of petiole color with purple genes was dominant and controlled by one pair of major gene，and also affected by a few minor genes and environmental factors．At the same time，taking infertility source green stem pakchoi controlled by multiple alleles as female parent，we imported the infertility gene into the black leaf greens komatsuna by methods of hybridization，backcross，self-ing and artificial selection for ideal strain，and transformed and developed fertile lines of male sterile AB lines type 1 greens komatsuna with purple.
petioles. Its economic characteristics are similar to the male parent.
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Received: 23 January 2013
Published: 10 April 2013
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