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| Construction and Expression of a Cucumber Lipoxygenase Gene CsLOX2 in
Prokaryotic Cells |
| CHEN Qiao，CHEN Shu-xia*，WANG Cong-ying，HAO Li-ning，WAN Xu-hua，SHEN Xiaoqing，
CHENG Zhi-hui |
College of Horticulture， Northwest A&F University， Key Laboratory of Horticultural Plant Germplasm Resources
Utilization in Northwest China， Yangling 712100， Shaanxi， China |
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Abstract The ORF of cucumber（Cucumis sativus L.）CsLOX2 gene was inserted into an
expression vector pGEX-4T-1 through PCR method using specific primers Yh1 and Yh2，and the
recombined expression vector p4t-LOX2 was constructed. The expression amount of fusion protein
was detected under different expressive conditions，such as different time，different temperature and
different concentration of IPTG. The 115 kD fusion protein was obtained and induced by IPTG and it
showed that CsLOX2 gene encoded a 89 kD protein after the 26 kD GST protein tag was removed．The
proper induction condition was 37 ℃ temperature，0.80 mmol·L-1 IPTG concentration and 10.5 h
inducing time.
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Received: 09 January 2013
Published: 26 March 2013
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