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| Studies on Pumpkin Genetic Transformation Mediated by Ovary Injection |
| ZHANG Yan-chao，GE Yu，YU Yang，XU Wen-long，BAI Yu-jiao，CUI Chong-shi，QU Shu-ping* |
| Horticulture College of Northeast Agricultural University，Harbin 150030，Heilongjiang，China |
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Abstract Taking pumpkin（Cucurbita moschata Duch.?ex Poir.）variety‘Yinhui No.?1’as receptor，aiiA gene was transferred into 148 pumpkin ovaries by injection．115 fruits were obtained with 77.7% seed setting percentage．Then 56 fruits were randomly selected，screened by 0.2% Basta aqueous solution and PCR identification．Finally，7 positive plants were all from one single fruit and all of them showed the hybridization signals by PCR-Southern Blot identification．The result indicated that the aiiA gene had been integrated into the genomic DNA of pumpkin‘Yinhui No.?1’with a converting rate of 0.25% and the converting rate per fruit was 6.36%.
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Received: 22 February 2012
Published: 05 April 2012
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