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| Mapping of Clubroot Resistance Gene in Chinese Cabbage |
| WANG Tong-tong，ZHANG Shu-jiang，ZHANG Shi-fan，LI Fei，ZHANG Hui，SUN Ri-fei* |
| Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China |
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Abstract In order to obtain molecular markers closely linked to the clubroot resistance gene in Chinese cabbage〔Brassica campestris L.ssp. pekinensis（Lour）Olsson〕，a segregating F2 population with 197 individuals was constructed by selfing of a F1 hybrid A00645，which is highly resistant to clubroot．Clubroot resistance tests and genetic analysis indicated that the inheritance of clubroot resistance in this material was controlled by a single dominant gene．Based on bulked segregating analysis（BSA）and InDel technique，a total of 720 pairs of InDel primers were screened between the resistant and susceptible bulks constructed in F2 population．After further tests among F2 individuals and linkage analysis by JoinMap4.0，9 InDel markers linked to clubroot resistance gene were obtained．The flanking markers BrID90269 and BrID11683 closely linked to the resistance gene were located on each side，at genetic distances of 2.0 cM and 2.5 cM，respectively．This clubroot resistance gene was mapped on Scaffold10，which is in linkage group A8 of Chinese cabbage．
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Received: 10 April 2012
Published: 02 May 2012
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