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| Analysis on Expression of Anthracnose Defense Enzyme Activity and HRGP Content in Phaseolus vulgaris L. at Seedling Stage |
| ZENG Ling-nan，XIAO Zi-heng，LI Cheng-yan，ZHANG Ting，HU Guo-fu* |
| Life Science College of Northeast Agricultural University，Harbin 150030，Heilongjiang，China |
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Abstract The susceptible bean（Phaseolus vulgaris L. var. chinensis Hort.）cultivar‘Laolaishao’and resistant cultivar‘Dayoudou’were taken as testing materials．The activities of 5 kinds defense enzymes（chitinase，beta-1，3-glucanase，SOD，POD and LOX）and HRGP content were detected and analyzed after the 2 cultivars infected by Anthracnose in different times（0，3，6，9，12 d）．The results showed that content of HRGP and the enzyme activities of chitinase，SOD，POD and LOX in resistant cultivar markedly increased with the increase of time course of infection and the disease expansion．The peak of their activities appeared earlier than in susceptible one．Compared with the susceptible cultivars，the activity of POD in resistant cultivar changed most significantly，while the content of HRGP changed with minimum amplitude．And the results of testing enzyme activity of beta-1，3-glucanase showed that the enzyme activity of resistant cultivar in 0 d was higher than susceptible cultivar，while after inoculation its enzyme activity of resistant cultivar change tendency was the same as the susceptible cultivar．
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Received: 16 December 2011
Published: 28 February 2012
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