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| Studies on Agro-bacterium-mediated Transformation of Potato with P2300-pa-8e Gene |
| YANG Zhi-chao1，ZHANG Li-li1，LU Cui-hua1*，DI Hong1，JIANG Li-li1，ZHANG Zheng-guo1，LIN Zhong-ping2，HU Yuan-lei2 |
| 1Agronomy College，Northeast Agricultural University，Harbin 150030，Heilongjiang，China|2School of Life Sciences，Peking University，Beijing 100871，China |
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Abstract P2300-pa-8e gene was originated from Bt185，which exists in insect resistance transcription factor．In this study，P2300-pa-8e gene was transferred into the potato（Solanum tuberosum L.）cultivars，‘Zaodabai’and‘Kexin No.18’by agro-bacterium-mediated transformation，and influence factors of genetic transformation were optimized．Microtuber discs were used as explants．19 resistant plants of‘Zaodabai’and 21 resistant plants of‘Kexin No.18’were obtained．Resistant plants were blot analyzed by PCR detection，of which 5 and 9 plants were positive．And these resistand plants were analyzed by Southern-blot，of which 4 and 6 plants had a positive amplification．Southern blot showed that single copies of the P2300-pa-8e gene were inserted into the genome of the transgenic plants．By the result of insect-resistant experiment，the transgenic materials showed resistance to coleopteran pests Holotrichia parallela and the larvae of Holotrichia oblita（Fald.）．
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Received: 10 May 2011
Published: 22 June 2011
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