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| Totipotency Expression of Plant Protoplast and Its Application in Brassica oleracea L. Vegetable Breeding |
| SHENG Xiao-guang，GU Hong-hui*，ZHAO Zhen-qing，YU Hui-fang，WANG Jian-sheng，ZHANG Xiao-hui |
| Institute of Vegetables，Zhejiang Academy of Agricultural Sciences |
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Abstract The isolated protoplasts，exposed cells without coated cell wall，own the totipotency to regenerate a whole plant under appropriate culture conditions．Separation，culture and reproduction technology of plant protoplast is a platform for plant breeding，gene engineering and cell physiology study．Therefore it is of important significance．This paper expounds the obtain，culture and regeneration etc key technology in the process of protoplast totipotency expression，and their application in Brassica oleracea vegetables breeding．Existing problems in protoplast technology were discussed．Suggestions were proposed for improving plant protoplast culture system and for this work in the near future．
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Received: 15 April 2011
Published: 11 June 2011
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