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| Cytology Observation on Process of Potato Leaves Infected by Alternaria solani |
| ZUO Yu-hu1，SHI Jie1，HAN Qing-mei2，KANG Zhen-sheng2，TAI Lian-mei1，LIU Tong1 |
| 1黑龙江八一农垦大学，黑龙江大庆 163319|2西北农林科技大学植物保护学院，陕西省农业分子生物学重点实验室，陕西杨凌 712100 |
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Abstract The infection process and ultra-structure of Alternaria solani on potato（Solanum tuberosum L.）leaves were observed by microscope，scanning and transmission electron microscope．The results showed that conidia began to germinate，and germ tube start to form 2 h after inoculation．Appressorium formation in the hypha tip was exhibited 6 h after inoculation．The germ tubes directly penetrated the host epidermal cells junctions and formed infection hypha on leaf of susceptive variety‘Dongnong 303’8 h after inoculation．Hyphal invasion of host cells spread to the adjacent cell expansion，and cell inclusions and all organelles have been almost completely digested 24 h after inoculation in susceptible variety．The infectious process in susceptible variety was approximately the same as in resistant variety‘Kexin No.1’，but the time of occurrence in resistant variety was later than that of susceptible variety．There was more hypha in host cell in susceptive variety than that in resistant variety．The defense response of thicken cell wall was observed in resistant variety 24 h after inoculation，but no defense response was seen in susceptive variety．These results suggested that resistance was effective to invasion and spreading by A. solani in resistance variety.
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Received: 27 March 2011
Published: 05 May 2011
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