|
|
|
| Construction of RNAi Expression Vector of Pectatelyase from Strawberry |
| QIAN Chun, HE Qiao, ZHAI Jun-peng, SONG Bo, XIA Bei-bei, ZHANG Xing-guo, LIANG Guo-lu* |
| College of Horticulture and Landscapal, Southwest University, Chongqing 400715, China |
|
|
|
|
Abstract In order to enhance the firmness of strawberry（Fragaria ananassa Duch.）and extend the shelf life, this experiment constructed RNA interference（RNAi）expression vector using a 382 bp conservative fragment of pectate lyase. The target gene was successfully transferred into strawberry via Agrobacterium. Two postitive transformants were detected through PCR analysis.
|
|
Received: 11 February 2010
|
|
|
|
Corresponding Authors:
LIANG Guo-lu
|
|
|
|
|
|
|
