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| In Vitro Plant Regeneration and SSR Detecting of Regenerated-plants in Pepper(Capsicum annuum L.) |
| Mohammad Daoud Eliassi,SHEN Huo-lin*,YANG Wen-cai*,TAN Fang,WANG Shuai,GUO Shuang |
| College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China |
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Abstract In vitro plant regeneration was achieved from 6 pepper lines(Cannuum L.).The effects of different types of explants including cotyledon(C),hypocotyls(H),two half cotyledons with hypocotyl(CS)and half of one cotyledon with hypocotyl(CH),medium,genotype,hormones and SSR detecting of regenerated plants were evaluated.The best medium for bud induction was MB5 with addition of 2.0 mg·L-1 TDZ,1.0 mg·L-1 IAA,and 4 mg·L-1 AgNO3.The induction rate was as high as 100 %.The best medium for bud elongation was MB5 with 3.0 mg·L-1 6-BA,0.5 mg·L-1 IAA,5.0 mg·L-1 AgNO3 and 2.0 mg·L-1 GA3.The highest rate of bud elongation was 87.5 %.Medium for rooting was MS with 0.2 mg·L-1 IAA and 0.1 mg·L-1 NAA.The rate for rooting was 100 %.Different types of explants had different abilities to bud induction and elongation.The CS was the best for induction.A total of 50 pairs of SSR primers were used to analyze generation variation in regenerated-plants and original plants.Twelve pairs of primers showed polymorphisms in plants regenerated from line‘Qiemen’,while no genetic variance was detected in 86 regenerated plants of line‘Fudijian’.
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Received: 01 March 2009
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