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| Studies on Agrobacterium-mediated Transformation of Potato with ODREB2B Gene |
| CUI Shao-bin1，2,DI Hong1,LU Cui-hua1*,YANG Zhi-chao1,LIN Zhong-ping2,HU Yuan-lei2 |
| 1Agronomy College,Northeast Agricultural University,Harbin 150030,Heilongjiang,China; 2The Heihe Branch of Heilongjiang Academy of Agricultural Sciences,Heihe 164300,Heilongjiang,China;3Life Sciences College,Peking University,Beijing 100871,China |
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Abstract ODREB2B gene was originated from Orychophragmus vidaceus which exist in adverse resistance transcription factor.In this study,ODREB2B gene was transferred into the potato cultivars‘Huangmazi’and‘Zhongshu No.3’by Agrobacterium-mediated transformation,and influence factors of genetic transformation were optimized.Microtuber discs were used as explants.33 resistant plants of‘Huangmazi’and 27 resistant plants of‘Zhongshu No.3’were obtained.Resistant plants were blot analysis by PCR-Southern,of which 16 and 14 plants had a positive amplification.PCR-Southern blot showed that single copies of the ODREB2B gene were inserted into the genome of the transgenic plants.
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Received: 10 March 2009
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