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| The Efficient Protocol for Transforming Antisense AcInv Genen into Potato |
| Qi Enfang,Zhang Jinwen,Wang Yihang |
| Potato Institute; Gansu Academy of Agricultural Sciences; Lanzhou 730070; Agronomy College; Gansu Agricultural University; Lanzhou 730070 |
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Abstract The potato cultivar Longshu No.3,which is sensitive to Agrobacterium tumefaciens,was used as the transformation receptor.The regeneration and transformation systems via Agrobacterium tumefaciens were studied.For stem callus induction,the optimal medium was MS+6-BA 2.5 mg·L-1+NAA 0.5 mg·L-1+GA3 5.0 mg·L-1+2,4-D 1.0 mg·L-1,and the optimal medium for differentiation was MS+6-BA 3.5 mg·L-1+NAA 1.0 mg·L-1+GA3 10.0 mg·L-1.For mini-tuber chip,the optimal regeneration medium was MS+ZT 2.0 mg·L-1+IAA 1.0 mg·L-1.The ...
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Received: 04 January 2007
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