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| Transformation and Expression of Chitinase Gene in Potato Protoplasts |
| Di Hong1，Xiao Zhimin2，Liu Zhaojun2 |
| Agriculture College，Northeast Agricultural University，Harbin 150030 |
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Abstract The Chitinase gene was transferred into potato cultivar Desiree by protoplast PEG transformation method.The results showed that DNA at concentration of 75 μg?mL-1 led to the highest transformation efficiency.The delayed-selection method was used.The result of PCR showed that three regenerated plants were positive.
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Received: 27 September 2007
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