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| Studies on Ovary Culture by Inter-specific Hybridization of Brassica oleracea var.botrytis and Brassica rapa ssp.chinensis |
| LIU Yun-xia;WANG Xiao-wu |
| Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China |
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Abstract Four inbred line of Brassica oleracea var.botrytis,082005,082009,082010 and 082063 were used as paternal plants and male sterile B.rapa ssp.chinensis h08015 was used as maternal plant.Crosses were made to produce inter-specific hybridization through ovary in vitro culture.The results showed that different developmental days of ovary,inoculation method，content of hormone and paternal genotype had significant effects on germination rate of hybrid seed.The ovary in vitro culture could get better results 6 days after pollination.Seed germination rate would be higher than that by other inoculation methods,when seeds were taken out of ovary 4 days after culturing and then continue to be cultured.The MS medium without hormone was better than the MS with 6-BA and NAA,and different paternal genotypes had different appropriate sampling times.To majority paternal genotypes,the best ovary culturing times were 6 days after pollination,but to individual paternal genotype,the appropriate culturing time was 10 days after pollination.
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Received: 10 October 2009
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Corresponding Authors:
LIU Yun-xia
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