To clarify differences in root free amino acid contents after inoculating the
resistant and susceptible cucumbers with Fusarium oxysporum，this paper conducted inoculation on these
cucumber materials taking sterile water as the contrast．8，12，16，20 days after inoculation，the dynamic
changes in root system free amino acid content were analyzed．The results showed that the total free amino
acid content of resistant material was on a declining trend，after Fusarium oxysporum inoculation．While，
that in susceptible material maintained an upward trend．The total free amino acid contents of resistant and
susceptible materials treated with Fusarium oxysporum inoculation were all increased than that of the contrast．
8，12，16 and 20 days after inoculation，the total free amino acid contents in resistant material increased by
9.37%，38.81%，33.09% and 25.72%，respectively．That in susceptible material increased by 19.89%，
49.49%，101.11% and 208.96%，respectively．The total free amino acid contents after Fusarium oxysporum
inoculation in susceptible ＞ resistant material．After inoculation，the changing rules of β–alanie，Ornithine，
Leucine，Aspartic acid，Phenylalanine，Lysine，Urea，Arginine and γ- aminobutyric contents in resistant
and susceptible materials were obviously different．