|
|
|
| Procurement of Transgenic Potato with AFP Gene and Preliminary Identification of Its Late Blight Resistance |
| REN Yu-xia1，CHEN Ling-na2，3，LIU Gui-zhen2，WANG Qing2，YANG Sheng-niu2，SHI Chun-hong4，LI Ting-ting4，HOU Lu-zhen2，CHEN Ji-feng1*，CHEN Zheng-hua2* |
| 1Bio-engineering Department of Zhengzhou University，Zhengzhou 450000，Henan，China|2Postdoctoral Scientific Research Station of Gansu Yasheng Industrial（Group）Co.，Ltd.，Beijing 100101，China|3College of Life Sciences and Biotechnology，Beijing Forestry University，Beijing 100083，China|4Beijing Qingshen Landscape Engineering Co.，Ltd.，Beijing 100012，China |
|
|
|
|
Abstract The aim of the study is to obtain the transgenic potato（Solanum tuberosum L.）lines conferring resistance to late blight．AFP（antifungal protein）gene was introduced into potato susceptible cultivar‘Shepody’via Agrobacterium tumefaciens-mediated transformation，using micro-tuber discs as explants．Four positive plantlets were obtained by PCR assay and the glyphosate selection．The micro-tubers were introduced in the dark and then planted in the field to grow into complete plants for the identification of disease resistance．Using detached leaf assay，4 transgenic plant lines were identified for late blight resistance．The result showed that their disease resistance was higher than the non-transgenic ones．
|
|
Received: 12 November 2011
Published: 05 January 2012
|
|
|
|
|
|
|
|
|
