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| Construction of ICE1 Gene Plant Expression Vector and Transformation into Tomato（Lycopercicon esculentum Mill.） |
| ZHANG YU，JIANG Xin-Mei*，YU Xi-Hong* |
| Vegetable Physiology and Installation Horticulture Laboratory，College of Horticulture，Northeast Agricultural University，Harbin 150030，China |
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Abstract This experiment was based on the plant expression vectorPCAMBIA3301，constructed the plant expressing vectors P3301-ICE1，Carrying an Arabidopsis thaliana ICE1 gene under the drive of CaMV35S promoter.the plasmid was transferred into tomato（Lycopersicon esculentum Mill）“cultivar Da Huang”via agrobacterium-mediated transformation.5 cultivar Da Huang with Kan-resistance were obtained，The results of test PCR amplifications of ICE1 gene and RT-PCR proved that obtain 3 positive plant of cultivar‘Da Huang’，positive plant rate was 60%.The transgenic tomato were treated with low temperature（4℃）stress for 72h，the content of MDA were apparently lowered，the content of Pro and the activityof CAT and POD were significant higher.compared with non-transgenic tomato.
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Received: 07 March 2010
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