辐照花粉诱导西葫芦单倍体
许利彩1,2 李海真2* 沈火林1 葛志东2,3
1中国农业大学农学与生物技术学院,北京 100193;2国家蔬菜工程技术研究中心,北京 100097;3新疆农业大学园艺学院
,新疆乌鲁木齐 830052
Studies on Squash(Cucurbita pepo L.)Haploid Plants Induced by Irradiated Pollen
XU Li-cai1,2 ,LI Hai-zheng2* ,SHEN Huo-lin1 ,GE Zhi-dong2,3
1Agriculture and Biotechnology College,China Agricultural University,Beijing 100193,China;2National
Engineering Research Center for Vegetables,Beijing 100097,China;3College of Horticulture,Xinjiang
Agricultural University,Urumchi 830052,Xinjiang,China
摘要
摘 要:利用不同剂量的60Co γ射线辐照西葫芦花粉后授粉,剥取不同发育阶段的胚接种在E20A培养基上进行培养,研究不
同基因型、辐照剂量、剂量率对西葫芦单倍体诱导的影响。结果发现:单倍体的产生和植株再生率受基因型、胚发育阶段和
辐照剂量的影响明显。281基因型平均单倍体诱导率最高,为1.30 %;点状胚和箭形胚易发育成单倍体苗;辐照剂量80~100
Gy时获得点状胚和箭形胚及胚总数最多,杆状胚的植株再生率和植株成活率最高,分别是83.33 %和35.71 %;适宜的剂量率
为45.19 Gy· min-1 。
关键词 :
西葫芦 ,
辐照花粉 ,
胚形态 ,
剂量率 ,
单倍体
Abstract :The influences of genotypes,gamma ray doses and dose-rate on the induced haploid embryos obtained
by irradiated pollen with different ray doses were studied in squash(Cucurbita pepo L.).Different embryo
shapes and development stages were found in seeds extracted from fruits cultured in medium E20A.The results
indicated that the rate of plants regeneration and haploid production were strongly influenced by
genotypes,embryo stages and gamma ray doses.The highest percentage of haploid production was 1.30 % for
genotypes 281.Point and arrow-like embryos were more likely to produce haploid plants.Meantime the percent of
point and arrow-like embryos and the numbers of all embryo shapes were the highest when their ray doses were
80-100 Gy.Stick-shape embryo gave the best result,in which the plants regeneration rate and survival
percentage were 83.33 % and 35.71 %,respectively.The optimum dose-rate was 45.19 Gy· min-1 .
收稿日期: 2009-01-14
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