Transfer of Para-carotene Biosynthesis Enzyme Gene(LycB) into Tomato
Kang Yanhong; Ji Jing; Wu Dong; et al.
Department of Agriculture Sciences; Hebei North University; Zhangjiakou 075131; College of Agriculture and Bioengineering; Tianjin University; Tianjin 300072; Institute of Chemical Defence
Abstract：The tomato genetic transformation system was established by cocultivated cotyledons of four varieties of tomato with agrobacterium tumefaciens EHA 101 including the plasmid pEbisLycBHyg.Then LycB gene can be transformed into tomato via agrobacterium tumefaciens.Regeneration plants of tomato were obtained by callus inducing,bud inducing,striking root cultivation and hygromycin screening in selective condition.PCR and PCR-Southern analysis of these resistant plants proved that LycB has been transferred into g...
收稿日期: 2005-08-05
引用本文:
抗艳红;季静;吴东;王罡. 类胡萝卜素合成酶基因LycB导入番茄的研究[J]. 中国蔬菜, 2006, 1(5): 11-13.
Kang Yanhong;Ji Jing;Wu Dong;et al.. Transfer of Para-carotene Biosynthesis Enzyme Gene(LycB) into Tomato. , 2006, 1(5): 11-13.
