SRAP and ISSR Analysis on Genetic Diversity of Onion Germplasm Resources
LIU Xiangping1，DU Minxia1，ZHAO Yan2，WANG Baosheng1，LIU Yan1，LIAN Yong1，DI Jiezeng1，#br#
XU Weizhong1
（1Institute of Vegetables，Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences，Huhhot 010031，Inner
Mongolia，China；2 College of Grassland，Resources and Environmental Science，Inner Mongolia Agricultural University，
Huhhot 010018，Inner Mongolia，China）
Abstract：Abstract：The genetic diversity of 58 onion germplasm resources was analyzed by SRAP and ISSR molecular
marker technology．Nine ISSR primers with better amplification effects were screened out and a total of 105
clear bands were amplified，including 104 bands with polymorphic sites．The ratio of polymorphic bands
was 99%．Eleven SRAP primers with better amplification effects were screened out and a total of 132 clear
bands were amplified，among which 115 were polymorphic loci．The ratio of polymorphic bands was 87%，
indicating 58 accessions of onion germplasm resource with abundant genetic diversity．The results of clustering
analysis based on 2 kinds molecular marker indicated that 58 accessions of onion germplasm resource could be
divided into 4 major categories．The results of these 2 clustering analysis were basically the same，but SRAP
molecular marker technology could detect more genetic locus，so it was the best molecular marker technology
for genetic diversity analysis．
