First Report of the Occurance of Tomato chlorosis virus in Hunan Province
WANG Xue-zhong1，2，ZHANG Zhan-hong3，ZHENG Li-min2，TANG Xin2，SHI Xiao-bin2，LIU Yong1，2*
（1 Subcollege of Longping，Graduate School of Hunan University，Changsha 410125，Hunan，China；2 Plant Protection
Institute of Hunan Province，Changsha 410125，Hunan，China；3 Hunan Vegetable Research Institute，Changsha
410125，Hunan，China）
During the investigation for vegetable diseases of Hunan Province in July 2016，symptoms of intervein chlorosis and leaf yellowing were discovered on Solanaceae vegetable leaves，including tomato，pepper，eggplant and potato plants. The symptoms were suspected as Tomato chlorosis virus（ToCV）and a large number of Bemisia tabaci were found on the back of leaves. The infected leaves of tomato，pepper，eggplant and potato and Bemisia tabaci samples were detected by RT-PCR using gene specific primers of ToCV heat shock protein 70 （HSP70）sequence，and an expected fragment were obtained. The results of sequence analysis revealed that the fragment shared the highest nucleotide identity at 99.0% with the isolates from Beijing（KC887999.1）. The infection rate of ToCV of these 4 kinds of Solanaceae vegetables was 70%-100%. Viruliferous rate of Bemisia tabaci was 66.7%-87.5%. The whitefly biotype was MED. This is the first time that Hunan Province confirms the existing of ToCV，which needs special attention and stronger prevention.
