Storage and transportation of vegetable commercial seedlings becomes an important link，along with the continuous expansion of facility vegetables planting areas and vegetable seedling culture industry．This paper took pepper variety‘ Nongda No.24’ as experimental material and studied on the changes in morphology and physiology indexes of plug seedling under different low temperature and poor light environment，aiming at finding suitable environment condition for long period storage and transportation of pepper seedlings．Results showed that no quality changes found in pepper plug seedlings during 14 days storage and transportation at 11 ℃ temperature in 30 μmol·m-2·s-1 light intensity and 12 h·d-1 photoperiod and using fluorescent lamp with 1.9 R∶B ratio and in 15 μmol·m-2·s-1 light intensity and 24 h·d-1 photoperiod using LED．Compared to fluorescent lamp，LED with advantages of high lighting efficiency and low energy consumption is more suitable for providing pepper seedlings with long-distance transportation and storage in low temperature and weak light intensity．