Chinese kale（Brassica alboglabra L. H. Bailey）is a thermophilic vegetable，which can’t well grow at high temperature．High temperaturewould prevent Chinese kale from growing and result in poor quality of its seedlings，seriously influence yield and quality in later period in summer and autumn．Taking 10 Chisnses kale lines as experimental materials，the growth indexs and physiology indexs under different high temperature stress were measured．The relative change rate of 10 Chinese kale lines under different high temperature stress was analyzed．The results showed that heat damage index and relative growth could be used to determine the heattolerance ability of Chinese kale lines，which can be most quick and convenient．The difference in heat tolerance among the varieties could be indicated by physiological and chemical index under 37℃ /25 ℃ or 40℃ /32 ℃． After the treatment of 37 ℃ for 1 hour the expression variation of MBF1c was increased．According to the comprehensive analysis，‘Aijiaoxianggu’ was the most heat-tolerant lines.